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yinzhijiedukeli for swine fever using method

  • A new assay for classical swine fever virus based on ...

    A new assay termed the dome disappearance method for classical swine fever virus (CSFV) using FS-L3 cells with serum-free culture medium was developed. The CSFV live vaccine GPE- strain grows well and shows a slight cytopathic effect (CPE) in FS-L3 cells. This CPE results in the disappearance of the …

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  • Method for producing swine fever live vaccine by

    2010-2-23 · The invention discloses a method for producing a swine fever live vaccine by using a porcine kidney cell line (IBRS-2). The method comprises the following steps of: (1) selecting a porcine kidney cell line (IBRS-2) as a cell for producing the vaccine (2) carrying out sub-culturing on the cell for producing the vaccine (3) reproducing virus cell seeds (4) reproducing venom for producing the ...

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  • Detection of African swine fever virus ASFV using Stilla ...

    2020-10-23 · Detection of African swine fever virus ASFV using Stilla Naica digital PCR method African swine fever (ASF, Africa Swine Fever) is an acute, highly contagious disease caused by the African swine fever virus. Due to the complexity of the etiology and epidemiology of the disease, and the lack of effective vaccines for epidemic prevention,

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  • A New Method for Detection African Swine Fever

    2021-6-1 · African swine fever (ASF) has severely influenced the swine industry of the whole world. Fast and accurate African swine fever virus (ASFV) antigen detection is very important for ASF prevention. This study aims to establish a new detection method for detection ASFV antigen using time-resolved fluorescence immunoassay (TRFIA) in the nose and mouth discharge.

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  • Detection of African swine fever virus in cell culture

    A sensitive immunocytochemical (IC) and immunohistochemical (IHC) method has been developed for detection of African swine fever (ASF) virus. • The IC test has the same sensitivity as the indirect fluorescent antibody test method using polyclonal antibodies.

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  • Epidemiological analysis of classical swine fever in wild ...

    2021-5-11 · Classical swine fever (CSF) is a contagious disease of pigs and wild boars that is transmitted through direct/indirect contact between animals or CSF virus-contaminated fomites. When the disease re-emerged in 2018 in Japan, a CSF-infected wild boar was reported shortly after the initial pig farm outbreak; subsequently, the disease spread widely.

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  • Managing African Swine Fever: Assessing the

    2021-6-15 · The recent spreading of African swine fever (ASF) over the Eurasian continent has been acknowledged as a serious economic threat for the pork industry. Consequently, an extensive body of research focuses on the epidemiology and control of ASF. Nevertheless, little information is available on the combined effect of ASF and ASF-related control measures on wild boar ( Sus scrofa ) population ...

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  • Detection of African swine fever virus by loop

    2010-3-1 · 1.. IntroductionAfrican swine fever virus (ASFV: genus Asfivirus, family Asfarviridae) is a large enveloped virus with a double-stranded DNA genome of between 170 kb and 190 kb.In sub-Saharan Africa, ASFV is maintained by long-term, inapparent infection of wildlife hosts such as bush pigs (Potamochoerus porcus) and warthogs (Phacochoerus africanus) which are infected via tick bites of …

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  • Development of a Rapid and Sensitive Method for

    African swine fever virus (ASFV) is an enveloped, complex, icosahedral virus with a double-stranded DNA genome of 170 to 190 kb in length (Takamatsu et al., 2013 16. Takamatsu HH, Denyer MS, Lacasta A, Stirling CM, Argilaguet JM, Netherton CL, Oura CA, Martins C, Rodriguez F. Cellular immunity in ASFV responses. Virus research 2013; 173: 110-121.

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  • A solid-phase enzyme linked immunosorbent assay

    1997-7-1 · ELSEVIER Journal of Virological Methods Journal of Virological Methods 66 (1997) 211-218 A solid-phase enzyme linked immunosorbent assay using monoclonal antibodies, for the detection of african swine fever virus antigens and antibodies Monica I. Vidaia'*, Matthias Stiene b, Jg Henkelb, Ursula Bilitewskib, Joao V. Costac, Abel G. Oliva a a Instituto de Biologin Experimental e Tecnologica …

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  • Method for producing swine fever live vaccine by

    2010-2-23 · The invention discloses a method for producing a swine fever live vaccine by using a porcine kidney cell line (IBRS-2). The method comprises the following steps of: (1) selecting a porcine kidney cell line (IBRS-2) as a cell for producing the vaccine (2) carrying out sub-culturing on the cell for producing the vaccine (3) reproducing virus cell seeds (4) reproducing venom for producing the ...

    Get Price
  • Detection of African swine fever virus by loop

    2010-3-1 · 1.. IntroductionAfrican swine fever virus (ASFV: genus Asfivirus, family Asfarviridae) is a large enveloped virus with a double-stranded DNA genome of between 170 kb and 190 kb.In sub-Saharan Africa, ASFV is maintained by long-term, inapparent infection of wildlife hosts such as bush pigs (Potamochoerus porcus) and warthogs (Phacochoerus africanus) which are infected via tick bites of …

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  • Disinfection of foot-and-mouth disease and African swine ...

    Disinfection of foot-and-mouth disease and African swine fever viruses with citric acid and sodium hypochlorite on birch wood carriers Peter W. Kruga,*, Christopher R. Larsona,b, Angelique C. Eslamia,b, Luis L. Rodrigueza aForeign Animal Disease Research Unit, United States Department of Agriculture, Agricultural Research Service, Plum Island Animal Disease Center, Greenport, NY

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  • Development of A Super-Sensitive Diagnostic Method

    African swine fever (ASF) is an infectious disease caused by African swine fever virus (ASFV) with clinical symptoms of high fever, hemorrhages and high mortality rate, posing a threat to the global swine industry and food security. Quarantine and control of ASFV is crucial for preventing swine industry from ASFV infection. In this study, a recombinase polymerase amplification (RPA)-CRISPR ...

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  • Classical swine fever virus: a ring test to evaluate RT ...

    2000-4-13 · Classical swine fever virus (CSFV) is a member of the Pestivirus genus within the family Flaviviridae. It is an enveloped virus with a single stranded, positive sense 12.5 kb RNA genome, flanked by non-coding regions (NCR) (Meyers and Thiel, 1996). The 5′ …

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  • Computational Analysis of African Swine Fever Virus ...

    2021-4-14 · Rapid detection of African swine fever virus using Cas12a-based portable paper diagnostics.Cell Discov.2020 Apr 7;6(1):18.doi: 10.1038/s41421-020-0151-5.

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  • Development of a Rapid and Sensitive Method for

    African swine fever virus is the etiological agent of African swine fever, a transmissible severe hemorrhagic disease that affects pigs, causing massive economic losses. There is neither a treatment nor a vaccine available, and the only method to control its spread is by extensive culling of pigs. So far, classical vaccine development approaches have not yielded sufficiently good results in ...

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  • [PDF] Development of A Super-Sensitive Diagnostic

    INTRODUCTION. African swine fever virus (ASFV) is an enveloped, complex, icosahedral virus with a double-stranded DNA genome of 170 to 190 kb in length (Takamatsu et al., 2013).It has been classified as the sole member of the new family Asfarviridae(Sanchez et al., 2013).This virus infects warthogs and bush pigs through bites from the argasid tick vector, as well as domesticated pigs and wild ...

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  • Method for producing swine fever live vaccine by

    2010-2-23 · The invention discloses a method for producing a swine fever live vaccine by using a porcine kidney cell line (PK15). The method comprises the following technical steps of: (1) selecting a porcine kidney cell line (PK15) as a cell for producing the vaccine (2) carrying out sub-culturing on the cell for producing the vaccine (3) reproducing virus cell seeds (4) reproducing venom for producing ...

    Get Price
  • [PDF] Simulation of a classical swine fever outbreak in ...

    Several different strategies for control and eradication of classical swine fever (CSF) were compared using a Monte Carlo method-based simulation model. The control strategy analysed in this paper, in addition to other CSF control measures, includes application of biosecurity measures on pig farms and rural backyard hold - ings. Elements of the control strategy are based on applicable ...

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  • Within- and between-pen transmission of Classical

    2002-4-25 · By using previously published data of experiments with Classical Swine Fever Virus more extensively, we obtained smaller confidence intervals than the martingale method used in the original papers. Moreover, our method allows simultaneous estimation of a reproduction ratio within pens R 0 w and a modified reproduction ratio between pens R ′ 0 b.

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  • Development of a Rapid and Sensitive Method for

    Brazilian Archives of Biology and Technology () . Development of a Rapid and Sensitive Method for Detection of African Swine Fever Virus Using Loop-Mediated Isothermal Amplification

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  • Electronic Supplementary Material Development of a

    2021-1-13 · Development of a Super-Sensitive Diagnostic Method for African Swine Fever Using CRISPR Techniques Meishen Ren 1,2 • Hong Mei • Ming Zhou • Zhen F. Fu1,2 • Heyou Han1,3 • Dingren Bi1,2 • Fuhu Peng4 • Ling Zhao1,2 1. State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China 2.

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  • African swine fever (ASF) detection and diagnosis

    2021-2-9 · African swine fever (ASF) is a contagious viral disease that causes a haemorrhagic fever in pigs and wild boar, and is often associated with lethality of up to 100 percent. As a result, ASF can severely impact on the productivity of pig farming. This not only threatens food security and challenges the …

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  • African and classical swine fever: similarities ...

    2017-11-28 · Introduction. Similar names suggest similar disease characteristics for African and classical swine fever (ASF and CSF). In fact, ASF was even thought to be caused by the same virus as CSF [] before Montgomery [] described it as an independent disease entity in Kenya.Yet, despite the similar clinical signs and some shared pathogenic characteristics, the two diseases are caused by completely ...

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  • Induction of Robust Immune Responses in Swine by

    2016-11-4 · The African swine fever virus (ASFV) causes a fatal hemorrhagic disease in domestic swine, and at present no treatment or vaccine is available. Natural and gene-deleted, live attenuated strains protect against closely related virulent strains; however, they are yet to be deployed and evaluated in the field to rule out chronic persistence and a potential for reversion to virulence. Previous ...

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  • Polymerase cross-linking spiral reaction (PCLSR) for ...

    2017-2-15 · African swine fever (ASF) is currently an issue of great importance for international trade of pigs and pig-origin meat products 1.ASF is an infectious viral disease of pigs, wild boars and other ...

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  • Experimental pig-to-pig transmission dynamics for

    2015-5-20 · African swine fever virus (ASFV) continues to cause outbreaks in domestic pigs and wild boar in Eastern European countries. To gain insights into its transmission dynamics, we estimated the pig-to-pig basic reproduction number (R 0) for the Georgia 2007/1 ASFV strain using a stochastic susceptible-exposed-infectious-recovered (SEIR) model with parameters estimated from transmission …

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  • Method for producing swine fever live vaccine by

    2010-2-23 · The invention discloses a method for producing a swine fever live vaccine by using a porcine kidney cell line (PK15). The method comprises the following technical steps of: (1) selecting a porcine kidney cell line (PK15) as a cell for producing the vaccine (2) carrying out sub-culturing on the cell for producing the vaccine (3) reproducing virus cell seeds (4) reproducing venom for producing ...

    Get Price
  • Application of an AlphaLISA method for rapid

    2021-5-29 · Infection with African swine fever virus (ASFV) causes an acute and highly lethal hemorrhagic disease that has been responsible for huge economic losses in China. To exactly detect the antigen of ASFV, we established a rapid, no-wash, one-step sandwich-type immunoassay based on the amplified luminescent proximity homogenous assay-linked immunosorbent assay (AlphaLISA) using …

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  • Within- and between-pen transmission of Classical

    2002-4-25 · By using previously published data of experiments with Classical Swine Fever Virus more extensively, we obtained smaller confidence intervals than the martingale method used in the original papers. Moreover, our method allows simultaneous estimation of a reproduction ratio within pens R 0 w and a modified reproduction ratio between pens R ′ 0 b.

    Get Price
  • [PDF] Simulation of a classical swine fever outbreak

    Several different strategies for control and eradication of classical swine fever (CSF) were compared using a Monte Carlo method-based simulation model. The control strategy analysed in this paper, in addition to other CSF control measures, includes application of biosecurity measures on pig farms and rural backyard hold - ings. Elements of the control strategy are based on applicable ...

    Get Price
  • Pathogenicity of an African swine fever virus strain ...

    2021-5-2 · African swine fever (ASF), caused by the ASF virus (ASFV), was first reported in Vietnam in 2019 and spread rapidly thereafter. Better insights into ASFV characteristics and early detection by surveillance could help control its spread. However, the pathogenicity and methods for early detection of ASFV isolates from Vietnam have not been established.

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  • African and classical swine fever: similarities ...

    2017-11-28 · Introduction. Similar names suggest similar disease characteristics for African and classical swine fever (ASF and CSF). In fact, ASF was even thought to be caused by the same virus as CSF [] before Montgomery [] described it as an independent disease entity in Kenya.Yet, despite the similar clinical signs and some shared pathogenic characteristics, the two diseases are caused by completely ...

    Get Price
  • Structural Glycoprotein E2 of Classical Swine Fever

    2021-5-24 · The classical swine fever virus (CSFV) glycoprotein E2 is the major structural component of the virus particle. E2 is involved in several functions, such as virus adsorption to the cell, the elicitation of protective immune responses, and virus virulence in swine. Using a yeast two-hybrid system, we previously identified the swine host protein Torsin-1A, an ATPase protein residing in the ...

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  • Vaccines | Free Full-Text | Standardized

    African swine fever (ASF) remains the most serious pig infectious disease, and its persistence in domestic pigs and wild boar (WB) is a threat for the global industry. The surveillance of WB plays a central role in controlling the disease and rapidly detecting new cases. As we are close to eradicating ASF, the need to find any possible pockets of infection is even more important.

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  • Enhanced discrimination of African swine fever virus ...

    2008-11-14 · Minimum evolution trees depicting a p72 protein and b p54 protein among 85 African swine fever virus isolates. The evolutionary history was inferred using the Minimum Evolution method (ME). The trees are drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree.

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  • Preliminary analysis of whether mosquitoes can

    2021-4-9 · Mosquitoes are important insect vectors, but whether they can carry and transmit African swine fever virus (ASFV) in large-scale pig farms in China is unknown. In this study, probe-based qPCR analysis was performed on mosquitoes from five pig farms with ASF virus (ASFV). Analysis of ASFV in 463 mosquitoes yielded negative cycle threshold (CT) value), and detection remained negative after ...

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  • Method for producing swine fever live vaccine by

    2010-2-23 · The invention discloses a method for producing a swine fever live vaccine by using a porcine kidney cell line (PK15). The method comprises the following technical steps of: (1) selecting a porcine kidney cell line (PK15) as a cell for producing the vaccine (2) carrying out sub-culturing on the cell for producing the vaccine (3) reproducing virus cell seeds (4) reproducing venom for producing ...

    Get Price
  • Managing African Swine Fever: Assessing the

    2021-6-15 · The recent spreading of African swine fever (ASF) over the Eurasian continent has been acknowledged as a serious economic threat for the pork industry. Consequently, an extensive body of research focuses on the epidemiology and control of ASF. Nevertheless, little information is available on the combined effect of ASF and ASF-related control measures on wild boar ( Sus scrofa ) population ...

    Get Price
  • [PDF] Simulation of a classical swine fever outbreak

    Several different strategies for control and eradication of classical swine fever (CSF) were compared using a Monte Carlo method-based simulation model. The control strategy analysed in this paper, in addition to other CSF control measures, includes application of biosecurity measures on pig farms and rural backyard hold - ings. Elements of the control strategy are based on applicable ...

    Get Price
  • Detection of African swine fever virus by loop

    2010-3-1 · 1.. IntroductionAfrican swine fever virus (ASFV: genus Asfivirus, family Asfarviridae) is a large enveloped virus with a double-stranded DNA genome of between 170 kb and 190 kb.In sub-Saharan Africa, ASFV is maintained by long-term, inapparent infection of wildlife hosts such as bush pigs (Potamochoerus porcus) and warthogs (Phacochoerus africanus) which are infected via tick bites of …

    Get Price
  • Rapid detection of African swine fever virus using Cas12a ...

    2021-4-14 · Rapid detection of African swine fever virus using Cas12a-based portable paper diagnostics.Cell Discov.2020 Apr 7;6(1):18.doi: 10.1038/s41421-020-0151-5.

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  • Comparative detection of classical swine fever virus

    Comparative detection of classical swine fever virus in striated muscle from experimentally infected pigs by reverse transcription polymerase chain reaction, cell culture isolation and immunohistochemistry. Thür B(1), Hofmann MA. Author information: (1)Institute of Virology and …

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  • Chimeric DNA/LNA-based biosensor for the rapid

    2018-7-1 · African swine fever (ASF) virus is a DNA virus responsible for a severe haemorrhagic fever in pigs, which (still in the absence of vaccination strategies) results in high mortality rates. Herein, we present a biosensor-based method for the detection of ASF viral DNA in the blood of pigs. The biosensor exploits a single-strand DNA probe with ...

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  • Synthesis of African swine fever (ASF) virus-specific ...

    De Boer CJ. Studies to determine neutralizing antibody in sera from animals recovered from African swine fever and laboratory animals inoculated with African virus with adjuvants. Arch Gesamte Virusforsch. 1967; 20 (2):164–179. Enjuanes L, Carrascosa AL, Moreno MA, Viñuela E. Titration of African swine fever (ASF) virus. J Gen Virol.

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  • Development of a chemiluminescence immunoassay to ...

    2021-3-1 · 3 33. Introduction. 34. African Swine Fever (ASF) is an acute, febrile, and highly contagious animal 35 infectious disease of pigs caused by African Swine Fever Virus (ASFV) (1, 2).Its 36 clinical symptoms mainly include skin congestion and cyanosis, severe bleeding of 37 internal organs, high morbidity, high mortality, and a short disease course (2, 3).

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  • Hunters’ acceptability of the surveillance system and ...

    2016-9-6 · The emergence of Classical Swine Fever (CSF) within a wild boar population is a highly undesirable event. This is mainly due to the potential transmissibility of the infectious agent from wild boar into domestic pig populations [1–3].Also, strict movement regulations affecting domestic pig holdings are implemented for the holdings in the restriction zone around a CSF outbreak according to EU ...

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  • Prevention and control of African swine fever - WUR

    2021-7-13 · The African swine fever virus can also be demonstrated with this test. The virus present in the blood or organs first has to be cultured on cells before it can be demonstrated using a colour method. At least five days are needed before virus isolation can provide an outcome. This test is used to confirm a first positive PCR in a region ...

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  • African swine fever diagnostics - WUR

    2021-7-14 · African swine fever (ASF) is a devastating disease of domestic pigs and wild boars caused by a DNA arbo-virus, African swine fever virus (ASFV), belonging to the family Asfarviridae. ASF is endemic in sub-Saharan Afri-can countries and has become more prevalent in the Cau-casus region since its spread from eastern Africa to Geor-gia in 2007.

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  • Phylogeographic Analysis of African Swine Fever Virus ...

    2012-3-7 · A highly sensitive and specific real-time PCR method was developed for the reliable and rapid detection of African swine fever virus (ASFV). The method uses a commercial Universal Probe Library (UPL) probe combined with a specifically designed primer set to amplify an ASFV DNA fragment within the VP72 coding genome region.

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  • Molecular Diagnosis of African Swine Fever by a

    1970-1-1 · Europe PMC is an archive of life sciences journal literature. [Immunohistological studies on swine fever. I. Method of localization of the swine pest virus in the organ tissue of experimentally infected swine using direct immunofluorescence in conjunction with Evan's blue contrast staining]

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  • [Immunohistological studies on swine fever. I.

    2021-3-19 · African swine fever virus (ASFV) causes hemorrhagic disease in domestic pigs by replicating mainly in monocyte/macrophage lineages. Various primary cells including pulmonary alveolar macrophages have been used for the propagation of ASFV on this account. However, ethical constraints and consistency problems exist as it is necessary to harvest same phenotype of primary cells in order …

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  • R0 Estimation for the African Swine Fever Epidemics

    2021-4-15 · This method is more fuel efficient than incineration as it uses gases produced during the combustion of the biological materials to fuel further combustion. Commercial adoption has been limited as the initial costs are high. ... 07-Jun-2021 African swine fever: The swine disease spread by humans.

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  • The Isolation and Replication of African Swine Fever

    2020-6-4 · African Swine Fever (ASF) is a highly infectious viral disease with high mortality. The most recent ASF outbreak in Vietnam occurred in 2019, posing a threat to spread to the neighboring Asian countries. Without a commercial vaccine or efficient chemotherapeutics successfully developed, rapid diagnosis and necessary biosecurity procedures are required to control the disease.

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  • Carcass disposal management for swine producers -

    2020-3-14 · African swine fever is a devastating disease that can result in death in almost all infected pigs. The continuing spread of African swine fever from Africa to Europe and recently to the high–pig production countries of China and others in Southeast Asia threatens global pork production and food security. The African swine fever virus is an unusual complex DNA virus and is not related to ...

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  • Molecular Diagnosis of African Swine Fever by a

    2012-3-7 · A highly sensitive and specific real-time PCR method was developed for the reliable and rapid detection of African swine fever virus (ASFV). The method uses a commercial Universal Probe Library (UPL) probe combined with a specifically designed primer set to amplify an ASFV DNA fragment within the VP72 coding genome region.

    Get Price
  • Assessment of African Swine Fever Diagnostic

    This study represents a complete comparative analysis of the most widely used African swine fever (ASF) diagnostic techniques in the European Union (EU) using field and experimental samples from animals infected with genotype II ASF virus (ASFV) isolates circulating in Europe. To detect ASFV, three different PCRs were evaluated in parallel using 785 field and experimental samples. The results ...

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  • Development of an indirect ELISA to specifically

    2021-5-5 · African swine fever (ASF), characterized by acute, severe, and fast-spreading, is a highly lethal swine infectious disease caused by the African swine fever virus (ASFV), which has caused substantial economic losses to the pig industry worldwide in the past 100 years. This study started with bioinformatics methods and verified the epitope fusion protein method's reliability that does not rely ...

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  • Induction of Robust Immune Responses in Swine by

    2016-11-4 · The African swine fever virus (ASFV) causes a fatal hemorrhagic disease in domestic swine, and at present no treatment or vaccine is available. Natural and gene-deleted, live attenuated strains protect against closely related virulent strains; however, they are yet to be deployed and evaluated in the field to rule out chronic persistence and a potential for reversion to virulence. Previous ...

    Get Price
  • Carcass disposal management for swine producers -

    2021-4-15 · This method is more fuel efficient than incineration as it uses gases produced during the combustion of the biological materials to fuel further combustion. Commercial adoption has been limited as the initial costs are high. ... 07-Jun-2021 African swine fever: The swine disease spread by humans.

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  • Direct From China, A Look at African Swine Fever ...

    2020-5-8 · This article was contributed by Linan Weng, Thermo Fisher Scientific, China. Since the first African swine fever (ASF) case was reported in China in August 2018, China has made significant upgrades to their pig industry, including implementing effective biosecurity measures and surveillance testing and progressively restructuring their industry. ...

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  • Detection of African swine fever virus in

    2020-12-22 · 1 INTRODUCTION. African swine fever virus (ASFV), the causative agent of an acute haemorrhagic fever of domestic and wild suids, was first identified in East Africa in the early 1900s (Dixon et al., 2019).In the last decade, African swine fever (ASF) has seen a remarkable expansion across Eurasia after an initial re-introduction in Georgia, followed by a spread through Russia and towards ...

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  • Development of a Rapid and Sensitive Method for

    African swine fever virus (ASFV) is an enveloped, complex, icosahedral virus with a double-stranded DNA genome of 170 to 190 kb in length (Takamatsu et al., 2013 16. Takamatsu HH, Denyer MS, Lacasta A, Stirling CM, Argilaguet JM, Netherton CL, Oura CA, Martins C, Rodriguez F. Cellular immunity in ASFV responses. Virus research 2013; 173: 110-121.

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  • Toward better control of classical swine fever in wild ...

    2020-7-31 · Classical swine fever (CSF) is a highly contagious viral disease caused by the CSF virus (CSFV), and is widely distributed around the world. CSFV belongs to the genus Pestivirus of the family Flaviviridae [].CSFV has three genotypes (1, 2 and 3) and several subgenotypes (1.1–1.4, 2.1–2.3, and 3.1–3.4) [2, 3], although a research group proposed recently a new classification schemes for ...

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  • RFID Tags for Use in Swine in PA - Penn State

    2020-5-6 · It is a method of permanent identification that can be read by an RFID reader or wand and can be read from a short distance. It is illegal to remove a tag. If, however, the animal loses a tag which sometimes does happen, you will need to go back to the original owner for a replacement. ... With African Swine Fever rampant across the world it is ...

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  • African swine fever diagnostics - WUR

    2021-7-13 · The African swine fever virus can also be demonstrated with this test. The virus present in the blood or organs first has to be cultured on cells before it can be demonstrated using a colour method. At least five days are needed before virus isolation can provide an outcome. This test is used to confirm a first positive PCR in a region ...

    Get Price
  • Molecular Diagnosis of African Swine Fever by a New

    2012-3-7 · A highly sensitive and specific real-time PCR method was developed for the reliable and rapid detection of African swine fever virus (ASFV). The method uses a commercial Universal Probe Library (UPL) probe combined with a specifically designed primer set to amplify an ASFV DNA fragment within the VP72 coding genome region.

    Get Price
  • VetMAX African Swine Fever Virus Detection Kit

    2020-7-7 · The VetMAX African Swine Fever Virus Detection Kit has successfully passed every step of the OIE procedure for the registration of diagnostic kits and has evidenced that it is fit-for-purpose for the detection of the African swine fever virus in blood, serum, and tissues of …

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  • R0 Estimation for the African Swine Fever Epidemics in ...

    2021-3-19 · African swine fever virus (ASFV) causes hemorrhagic disease in domestic pigs by replicating mainly in monocyte/macrophage lineages. Various primary cells including pulmonary alveolar macrophages have been used for the propagation of ASFV on this account. However, ethical constraints and consistency problems exist as it is necessary to harvest same phenotype of primary cells in order …

    Get Price
  • The Isolation and Replication of African Swine Fever

    Efficient and stable rescue of classical swine fever virus from cloned cDNA using an RNA polymerase II system. Li C(1), Huang J, Li Y, He F, Li D, Sun Y, Han W, Li S, Qiu HJ. Author information: (1)State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street ...

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  • Efficient and stable rescue of classical swine fever virus ...

    2021-3-26 · African swine fever, DNA capture, laboratory diagnosis, without DNA extraction Introduction African swine fever (ASF) is an extremely contagious disease of wild boar and domestic pigs with an almost 100% mortality rate, causing signi cant economic trauma to the pig industry in a ected countries (Dixon et al., 2020).

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  • Speci c High-sensitivity Multiple-probe-assisted DNA ...

    2020-12-21 · viruses Article Live Attenuated African Swine Fever Viruses as Ideal Tools to Dissect the Mechanisms Involved in Cross-Protection Elisabeth Lopez 1, Juanita van Heerden 2, Laia Bosch-Camós 1, Francesc Accensi 1,3, Maria Jesus Navas 1, Paula López-Monteagudo 1, Jordi Argilaguet 1, Carmina Gallardo 4, Sonia Pina-Pedrero 1, Maria Luisa Salas 5, Jeremy Salt 6 and Fernando Rodriguez 1,*

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  • Live Attenuated African Swine Fever Viruses as Ideal Tools ...

    2021-3-5 · African swine fever is an infectious, hemorrhagic-arboviral disease affecting domestic pig populations with a significant economic impact due to the direct loss of animals with up to 100% mortality rates (Jori and Bastos, 2009; Galindo and Alonso, 2017). It is caused by the Af-rican swine fever virus (ASFV), a double-stranded DNA virus belonging

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  • Detection and genetic characterization of African swine ...

    2018-8-9 · Direct molecular cloning of full-length cDNAs derived from viral RNA is an approach to identify the individual viral genomes within a virus population. This enables characterization of distinct viral haplotypes present during infection. In this study, we recover individual genomes of classical swine fever virus (CSFV), present in a pig infected with vKos that was rescued from a cDNA clone ...

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