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yinzhijiedukeli for swine circovirus infection using method

  • A novel method for the detection of porcine circovirus ...

    A novel method for the detection of porcine circovirus type 2 replicative double stranded viral DNA and nonreplicative single stranded viral DNA in tissue sections. Hamberg A(1), Ringler S, Krakowka S. Author information: (1)Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210, USA. [email protected]

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  • Porcine circovirus type 2 infection attenuates the K63 ...

    2021-7-1 · Porcine circovirus 2 (PCV2) has been proved to increase the risk of other pathogens infection via immunosuppression. Although the co-infection of PCV2 and porcine parvovirus (PPV) is commonly observed in worldwide, the relative immune mechanisms promoting PPV infection in PCV2-infected piglets are currently unknown.

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  • Fast Diagnosis and Quantification for Porcine

    2010-4-1 · The postweaning multisystemic wasting syndrome, caused by the porcine circovirus type 2 (PCV-2), is a major disease that poses a significant threat to the global swine industry. The purpose of this study was to establish a real-time polymerase chain reaction (PCR) method for the quantification of PCV-2 and to enable the rapid differentiation of porcine circoviruses type 1 and 2 (PCV-1 and PCV-2).

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  • Preclinical Detection of Porcine Circovirus Type 2 ...

    2017-4-14 · Porcine circovirus type 2 (PCV2) has emerged as one of the most important pathogens affecting swine production globally. Preclinical identification of PCV2 is very important for effective prophylaxis of PCV2-associated diseases. In this study, we developed an ultrasensitive nanoparticle DNA probe-based PCR assay (UNDP-PCR) for PCV2 detection. Magnetic

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  • Assays for the Detection of Porcine Circovirus (PCV) in ...

    2018-9-21 · Assays for the Detection of Porcine Circovirus (PCV) in Biological Samples Background on Circoviruses The first Circovirus was described in 1974 (I. Tischer) as a contaminant of porcine kidney cells in culture, replicating best in PK15 cells (ATCC - CCL 33). This virus was named Porcine Circovirus, or PCV. Since then, several similar viruses have been described that infect other

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  • Development of real-time PCR assay for detection of ...

    P1 infection can cause symptoms resembling postweaning multisystemic wasting syndrome. This study aims to develop a rapid, sensitive and specific method to detect P1. Results: A pair of primers was designed and used to amplify a 119 bp DNA fragment to generate a recombinant plasmid which was served as the standard. A SYBR I qPCR protocol was established using the P1 recombinant plasmid standard and the sensitivity, specificity and stability of this method …

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  • A survey on porcine circovirus type 2 infection and ...

    Porcine circovirus type 2 (PCV2) is closely related to the postweaning multisystemic wasting syndrome (PMWS). In this study, the pig serum and tissue samples collected from different regions of Hangzhou District in Zhejiang Province of China between 2003 and 2005 were analyzed by enzyme-linked immunosorbent assay (ELISA) for PCV2 antibody and by polymerase chain reaction (PCR) for ORF2 …

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  • Culturing and molecular methods to assess the

    2012-12-1 · The collected samples were clarified and concentrated using the glycine buffer method with polyethylene glycol precipitation, as described by Schlindwein et al. (2010). Briefly viral particles were eluted from samples by glycine buffer (pH 9.5) and further concentrated by PEG 6000 precipitation.

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  • Porcine circovirus type 2 (PCV2) infection in Hebei ...

    2021-5-24 · Porcine circovirus 2 (PCV2) is an icosahedral, non-enveloped, single-stranded circular DNA virus that belongs to the family Circoviridae, genus Circovirus . This genus includes PCV1, PCV2, and PCV3. PCV1 is a cell-culture-derived virus that is not pathogenic to swine.

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  • Rapid detection of Porcine circovirus 2 by

    RPA is performed at a constant temperature and therefore can be carried out in a water bath. In addition, RPA is completed in ~30 min, much faster than PCR, which usually takes >60 min. We developed a RPA-based method for the detection of PCV-2. The detection limit of …

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  • Characterization of porcine circovirus type 2 (PCV2 ...

    2008-8-15 · 1. Vet Immunol Immunopathol. 2008 Aug 15;124(3-4):355-66. doi: 10.1016/j.vetimm.2008.04.019. Epub 2008 May 4. Characterization of porcine circovirus type 2 (PCV2) infection in swine lymphocytes using mitogen-stimulated peripheral blood lymphocytes from healthy PCV2-carrier pigs.

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  • Porcine circovirus type 2 (PCV2) infection in Hebei ...

    2021-5-24 · Porcine circovirus type 2 (PCV2) is the primary causative agent of porcine circovirus-associated diseases in swine, the most common of which are postweaning multisystemic wasting syndrome (PMWS) and porcine dermatitis and nephropathy syndrome (PDNS). To investigate the prevalence and genetic diversity of PCV2 in Hebei Province, Northern China, from 2016 to 2019, a …

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  • [Case report: Porcine circovirus type 2 infection in an ...

    Bacteriology revealed a septicemic Salmonella choleraesuis var. Kunzendorf--infection. Virologically, the animal was tested with negative results for Classical Swine Fever Virus and PRRSV. The unusual depletion of the lymphatic tissue mainly in the spleen led to the suspicion of a PCV-2 infection.

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  • Fast Diagnosis and Quantification for Porcine

    2010-4-1 · The postweaning multisystemic wasting syndrome, caused by the porcine circovirus type 2 (PCV-2), is a major disease that poses a significant threat to the global swine industry. The purpose of this study was to establish a real-time polymerase chain reaction (PCR) method for the quantification of PCV-2 and to enable the rapid differentiation of ...

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  • Characterization of porcine circovirus type 2 (PCV2 ...

    2008-8-15 · Information regarding the susceptibility of swine lymphocytes to PCV2 is rather limited. To further explore and characterize the PCV2 infection in swine lymphocytes, an in vitro model using concanavalin A (Con A)-stimulated peripheral blood lymphocytes (PBLs) obtained from clinically healthy PCV2-carrier pigs was introduced. It was found that the PCV2 antigen-containing rate was below 2% …

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  • Development of real-time PCR assay for detection of ...

    Background: The porcine circovirus-like agent P1 is a newly discovered DNA virus with a single-stranded circular genome that is highly homologous to that of porcine circovirus type 2. P1 infection can cause symptoms resembling postweaning multisystemic wasting syndrome. This study aims to develop a rapid, sensitive and specific method to detect P1.

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  • Porcine circovirus type 2 infection attenuates the K63 ...

    2021-7-1 · Porcine circovirus 2 (PCV2) has been proved to increase the risk of other pathogens infection via immunosuppression. Although the co-infection of PCV2 and porcine parvovirus (PPV) is commonly observed in worldwide, the relative immune mechanisms promoting PPV infection in PCV2-infected piglets are currently unknown.

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  • Detection of porcine circovirus type 1 in commercial

    2006-5-1 · The absence of extraneous viruses is a requirement in the quality control of vaccines for veterinary use in the European Pharmacopoeia. A polymerase chain reaction (PCR) assay for the detection of porcine circovirus type 1 (PCV1) and type 2 (PCV2) was …

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  • Porcine Circovirus - an overview | ScienceDirect Topics

    Porcine circovirus (family Circoviridae, genus Circovirus) is a nonenveloped RNA virus, the smallest virus to infect mammals. Since its initial discovery in cell culture in 1974, another serotype has been discovered (PCV2) which causes disease in vivo, and has been further divided into …

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  • Pigeon Circovirus - an overview | ScienceDirect Topics

    Pigeon circovirus (PiCV) has been classified under the family Circoviridae, characterized by small non-enveloped single-stranded DNA virus ( Mankertz et al., 2000 ). Circovirus infection is mostly observed in birds such as geese, pigeons, ostriches, psittacines, gulls, and ducks ( Todd, 2004; Shen et al., 2015 ).

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  • A Novel Method for the Detection of Porcine Circovirus ...

    2019-9-1 · A novel method for the detection of porcine circovirus type 2 replicative double stranded viral DNA and nonreplicative single stranded viral DNA in tissue sections Alexander Hamberg,1 Susan Ringler, Steven Krakowka Abstract. Porcine circovirus type 2 (PCV2), an economically important pathogen of swine, is the necessary

    Get Price
  • Fast Diagnosis and Quantification for Porcine Circovirus ...

    2017-2-9 · circovirus type 2 (PCV-2), is a major disease that poses a significant threat to the global swine industry. The purpose of this study was to establish a real-time polymerase chain reaction (PCR) method for the quanti-fication of PCV-2 and to enable the rapid differentiation of porcine circoviruses type 1 and 2 (PCV-1 and PCV-2).

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  • Porcine circovirus 2 (PCV2) population study in ...

    2020-10-20 · Porcine circovirus 2 (PCV2) is a single stranded DNA virus with one of the highest mutation rates among DNA viruses. This ability allows it to generate a cloud of mutants constantly providing new ...

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  • Fast diagnosis and quantification for porcine

    Background/Purpose: The postweaning multisystemic wasting syndrome, caused by the porcine circovirus type 2 (PCV-2), is a major disease that poses a significant threat to the global swine industry. The purpose of this study was to establish a real-time polymerase chain reaction (PCR) method for the quantification of PCV-2 and to enable the rapid differentiation of porcine circoviruses type 1 ...

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  • Development of an in situ assay for simultaneous

    2011-1-24 · In cultured cells the average number of signals increased with time after infection. CONCLUSIONS: We have developed a method for detection of both strands of PCV2 in situ that can be useful for studies of replication and in situ detection of PCV2 as well as of DNA viruses in general. PMCID: PMC3033839 PMID: 21261961 [Indexed for MEDLINE]

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  • Evaluation of natural porcine circovirus type 2 (PCV2 ...

    This invention relates to a method for rapid production of PCV2 such that an optimum yield of the virus can be obtained. This invention also relates to the vaccine useful against PCV2 which includes the PCV2 propagated using such a method.

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  • WO2016183423A1 - Method for porcine circovirus

    2019-9-5 · A diagnostic method to simultaneously detect and discriminate porcine circovirus type 1 (PCV1), porcine circovirus type 2 (PCV2) and porcine circovirus type 3 (PCV3) in clinical specimens is imperative for the differential diagnosis and monitoring and control of PCVs in the field. Three primer pairs were designed and used to develop a multiplex PCR assay.

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  • Development of a multiplex PCR to detect and

    2018-8-27 · Detection of porcine circoviruses in clinical specimens using multiplex PCR in Hubei, central China 1 Keli Yang1,2, Zuwu Jiao1, Danna Zhou1,3, Rui Guo1, Zhengying Duan1, 2 Fangyan Yuan1 and Yongxiang Tian1,2 3 1Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan 430064, P. R. 4 China 5 2Key Laboratory of Prevention and Control Agents for …

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  • Detection of porcine circoviruses in clinical specimens ...

    2019-7-14 · Single infection with PCV3 accounted for only one-third of the samples, and majority were of co-infections, predominantly with PEDV (41.6%) but generally low with other swine viruses. Indirect ELISA using the PCV3 capsid protein as the coating antigen revealed an average sero-positive rate of 52.6% (40.8 to 60.8%) in 2345 serum samples from ...

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  • A Novel Method for the Detection of Porcine Circovirus ...

    2019-9-1 · A novel method for the detection of porcine circovirus type 2 replicative double stranded viral DNA and nonreplicative single stranded viral DNA in tissue sections Alexander Hamberg,1 Susan Ringler, Steven Krakowka Abstract. Porcine circovirus type 2 (PCV2), an economically important pathogen of swine, is the necessary

    Get Price
  • Detection of porcine circovirus genotypes 2a and 2b

    2012-5-3 · Porcine circovirus type 2 (PCV2) is a member of the family Circoviridae and the genus Circovirus. PCV2 is further grouped into genotypes a, b and c. PCV2a and PCV2b occur worldwide, while PCV2c was recently detected in archival samples in Denmark …

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  • Porcine Circovirus Diseases - Generalized Conditions ...

    2017-8-29 · erated using the maximum likelihood method and visualized using the program MEGA version 6 [15]. Bootstrap values (based on 100 replicates) for each node are shown if >70 %. Case 1 tissue virome and PCV3 genome Using a BLASTx E-score cutoff of 10 −5, we identified 50 se-quences related to circovirus-like SFBeef and PorkNW2 USA/2009 sequences.

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  • Detection of a novel circovirus PCV3 in pigs with cardiac ...

    2017-2-9 · circovirus type 2 (PCV-2), is a major disease that poses a significant threat to the global swine industry. The purpose of this study was to establish a real-time polymerase chain reaction (PCR) method for the quanti-fication of PCV-2 and to enable the rapid differentiation of porcine circoviruses type 1 and 2 (PCV-1 and PCV-2).

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  • Fast Diagnosis and Quantification for Porcine Circovirus ...

    2011-3-18 · Porcine circovirus type 2 (PCV2) is a non-enveloped, circular, single-stranded DNA virus that belongs to the Circoviridiae family [].This virus is widespread in the commercial swine population, and is accepted as the causative agent of a number of diseases in these animals, such as postweaning multisystemic wasting syndrome (PMWS) [], and porcine dermatitis and nephropathy syndrome (PDNS).

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  • Specific, simple and rapid detection of porcine

    2019-10-15 · Porcine circovirus type 2 (PCV2) is the aetiological agent of PCV2-Systemic Disease (PCV2-SD) and PCV2-Subclinical Infection (PCV2-SI). PCV2 is highly resistant to environmental conditions, being ...

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  • Environmental distribution of Porcine Circovirus Type

    Background/Purpose: The postweaning multisystemic wasting syndrome, caused by the porcine circovirus type 2 (PCV-2), is a major disease that poses a significant threat to the global swine industry. The purpose of this study was to establish a real-time polymerase chain reaction (PCR) method for the quantification of PCV-2 and to enable the rapid differentiation of porcine circoviruses type 1 ...

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  • Fast diagnosis and quantification for porcine

    This invention relates to a method for rapid production of PCV2 such that an optimum yield of the virus can be obtained. This invention also relates to the vaccine useful against PCV2 which includes the PCV2 propagated using such a method.

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  • WO2016183423A1 - Method for porcine circovirus

    Rapid detection of food-borne Salmonella contamination using IMBs-qPCR method based on pagC gene. Brazilian Journal of Microbiology , 2017.10:3241-9. l Menglu Shen, Yi Li, Linlin Zhang, Songbao Dai, Jiashun Wang, Yongqing Li, Lei Zhang*, Jinhai Huang * , Staphylococcal enterotoxin Profile of China Isolates and the Superantigenicity of Some Novel Enterotoxins , Archives of Microbiology ...

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  • Porcine Circovirus Diseases - Generalized Conditions ...

    2019-9-1 · A novel method for the detection of porcine circovirus type 2 replicative double stranded viral DNA and nonreplicative single stranded viral DNA in tissue sections Alexander Hamberg,1 Susan Ringler, Steven Krakowka Abstract. Porcine circovirus type 2 (PCV2), an economically important pathogen of swine, is the necessary

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  • A Novel Method for the Detection of Porcine Circovirus ...

    Porcine circovirus is an ubiquitous virus, present in swine-raising countries over the world. There are 2 types of virus: Porcine circovirus type 1 is non-pathogenic and has been identified as a cell culture contaminant whereas Porcine Circovirus type 2 (PCV2) is the one affecting pigs worldwide.

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  • Porcine Circovirus Associated Diseases (PCVAD) –

    2012-5-3 · Porcine circovirus type 2 (PCV2) is a member of the family Circoviridae and the genus Circovirus. PCV2 is further grouped into genotypes a, b and c. PCV2a and PCV2b occur worldwide, while PCV2c was recently detected in archival samples in Denmark …

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  • Detection of porcine circovirus genotypes 2a and 2b

    2019-10-15 · Porcine circovirus type 2 (PCV2) is the aetiological agent of PCV2-Systemic Disease (PCV2-SD) and PCV2-Subclinical Infection (PCV2-SI). PCV2 is highly resistant to environmental conditions, being ...

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  • Environmental distribution of Porcine Circovirus Type

    PORCINE CIRCOVIRUS 3 . SUMMARY . Etiology • Porcine circovirus (PCV) is a non-enveloped DNA virus with a single-stranded circular genome. It belongs to the genus Circovirus in the family Circoviridae. • Two species, PCV1 and PCV2, are currently recognized.

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  • PORCINE CIRCOVIRUS 3 - Iowa State University

    A loop-mediated isothermal amplification (LAMP) method with a real-time monitoring system was developed for the detection of porcine circovirus type 1 (PCV1) in commercial swine vaccines. This method was highly specific for PCV1. No cross-reaction to porcine circovirus type 2, porcine parvovirus, pseudorabies virus, classical swine fever virus, and porcine reproductive and respiratory syndrome ...

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  • [PDF] Detection of porcine circovirus type 1 in

    2019-9-5 · A diagnostic method to simultaneously detect and discriminate porcine circovirus type 1 (PCV1), porcine circovirus type 2 (PCV2) and porcine circovirus type 3 (PCV3) in clinical specimens is imperative for the differential diagnosis and monitoring and control of PCVs in the field. Three primer pairs were designed and used to develop a multiplex PCR assay.

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  • Development of a multiplex PCR to detect and

    2018-10-26 · Porcine circovirus is a small non-enveloped virus with a circular, single-stranded DNA genome, belonging to the family Circoviridae [].Porcine circovirus 1 (PCV1) was initially identified in the 1970s as a contaminating agent in pig kidney cells and was considered nonpathogenic for swine [2, 3].Porcine circovirus type 2 (PCV2), known as an essential pathogen of porcine circovirus-associated ...

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  • Molecular detection and genomic characterization of ...

    This invention relates to a method for rapid production of PCV2 such that an optimum yield of the virus can be obtained. This invention also relates to the vaccine useful against PCV2 which includes the PCV2 propagated using such a method.

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  • Fast Diagnosis and Quantification for Porcine Circovirus ...

    2017-2-9 · circovirus type 2 (PCV-2), is a major disease that poses a significant threat to the global swine industry. The purpose of this study was to establish a real-time polymerase chain reaction (PCR) method for the quanti-fication of PCV-2 and to enable the rapid differentiation of porcine circoviruses type 1 and 2 (PCV-1 and PCV-2).

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  • Development and validation of an ELISA using a

    2010-10-19 · A simple and reliable diagnostic method based on ORF2 protein immunoreactivity would serve as a valuable diagnostic method for detecting serum antibodies to PCV2 and monitoring PCV infection. Here, we reported an indirect enzyme-linked immunosorbent assay (I-ELISA) by using an antigenic domain (113-147AA) of ORF2-encoded antigen, expressed in E ...

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  • Detection of a novel circovirus PCV3 in pigs with cardiac ...

    2017-8-29 · erated using the maximum likelihood method and visualized using the program MEGA version 6 [15]. Bootstrap values (based on 100 replicates) for each node are shown if >70 %. Case 1 tissue virome and PCV3 genome Using a BLASTx E-score cutoff of 10 −5, we identified 50 se-quences related to circovirus-like SFBeef and PorkNW2 USA/2009 sequences.

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  • Development of an in situ assay for simultaneous

    2011-1-24 · In cultured cells the average number of signals increased with time after infection. CONCLUSIONS: We have developed a method for detection of both strands of PCV2 in situ that can be useful for studies of replication and in situ detection of PCV2 as well as of DNA viruses in general. PMCID: PMC3033839 PMID: 21261961 [Indexed for MEDLINE]

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  • A Novel Porcine Circovirus Distantly Related to Known ...

    IMPORTANCE While porcine circovirus 2 (PCV2) was first identified in sporadic cases of postweaning multisystemic wasting syndrome in Canada in the early 1990s, an epidemic of severe systemic disease due to PCV2 spread worldwide in the ensuing decade. Despite being effectively controlled by commercial vaccines, PCV2 remains one of the most economically significant viruses of swine.

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  • Development of a multiplex PCR to detect and

    2019-9-5 · porcine circovirus type found in swine, which is circulat-ing in the swine population [8]. The co-infection of PCV3 with PCV2 was reported in clinical samples of dis-eased pigs in Hubei province [2]. And co-infection of PCV2 with PCV1 was found in Hubei province [19]. However, PCV1, PCV2 and PCV3 or their co-infections

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  • Detection of porcine circoviruses in clinical specimens ...

    2018-8-27 · Detection of porcine circoviruses in clinical specimens using multiplex PCR in Hubei, central China 1 Keli Yang1,2, Zuwu Jiao1, Danna Zhou1,3, Rui Guo1, Zhengying Duan1, 2 Fangyan Yuan1 and Yongxiang Tian1,2 3 1Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan 430064, P. R. 4 China 5 2Key Laboratory of Prevention and Control Agents for …

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  • Porcine circovirus 2 (PCV2) population study in ...

    2020-10-20 · Porcine circovirus 2 (PCV2) is a single stranded DNA virus with one of the highest mutation rates among DNA viruses. This ability allows it to generate a cloud of mutants constantly providing new ...

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  • Immunologic Features of Porcine Circovirus Type 2

    2019-9-9 · Clinical expression of porcine circovirus 2 (PCV-2) infection in swine may result in two distinct high mortality disease syndromes. In North America, postweaning multisystemic wasting syndrome (PMWS), while still sporadic in incidence, predominates.

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  • Co-Infection of Swine with Porcine Circovirus Type 2

    2019-2-21 · 2. Co-Infection with Different Porcine Circovirus 2 (PCV2) Strains. PCV2 is divided into five genotypes according to the Cap gene sequence: PCV2a, 2b, 2c, 2d, and 2e [2,21].Moreover, the PCV2b genotype is classified into three clusters, 1A to 1C, and the PCV2a genotype is subdivided into five clusters, 2A to 2F [2,22,23,24].Recently, a retrospective study of PCV2 infection between 1996 and ...

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  • Fast Diagnosis and Quantification for Porcine Circovirus ...

    2017-2-9 · circovirus type 2 (PCV-2), is a major disease that poses a significant threat to the global swine industry. The purpose of this study was to establish a real-time polymerase chain reaction (PCR) method for the quanti-fication of PCV-2 and to enable the rapid differentiation of porcine circoviruses type 1 and 2 (PCV-1 and PCV-2).

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  • Porcine circovirus 2 infection in swine foetuses ...

    SANCHEZ, RE, Hans Nauwynck, F MCNEILLY, GM ALLAN, and Maurice Pensaert. 2001. “Porcine Circovirus 2 Infection in Swine Foetuses Inoculated at Different Stages of Gestation.” Veterinary Microbiology 83 (2): 169–176.

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  • Detection of a novel circovirus PCV3 in pigs with cardiac ...

    2017-8-29 · erated using the maximum likelihood method and visualized using the program MEGA version 6 [15]. Bootstrap values (based on 100 replicates) for each node are shown if >70 %. Case 1 tissue virome and PCV3 genome Using a BLASTx E-score cutoff of 10 −5, we identified 50 se-quences related to circovirus-like SFBeef and PorkNW2 USA/2009 sequences.

    Get Price
  • Environmental distribution of Porcine Circovirus Type

    2019-10-15 · Porcine circovirus type 2 (PCV2) is the aetiological agent of PCV2-Systemic Disease (PCV2-SD) and PCV2-Subclinical Infection (PCV2-SI). PCV2 is highly resistant to environmental conditions, being ...

    Get Price
  • Development of a multiplex PCR to detect and

    2019-9-5 · porcine circovirus type found in swine, which is circulat-ing in the swine population [8]. The co-infection of PCV3 with PCV2 was reported in clinical samples of dis-eased pigs in Hubei province [2]. And co-infection of PCV2 with PCV1 was found in Hubei province [19]. However, PCV1, PCV2 and PCV3 or their co-infections

    Get Price
  • Specific, simple and rapid detection of porcine

    2011-3-18 · Porcine circovirus type 2 (PCV2) is a non-enveloped, circular, single-stranded DNA virus that belongs to the Circoviridiae family [].This virus is widespread in the commercial swine population, and is accepted as the causative agent of a number of diseases in these animals, such as postweaning multisystemic wasting syndrome (PMWS) [], and porcine dermatitis and nephropathy syndrome (PDNS).

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  • Development and validation of an ELISA using a

    2010-10-19 · The capsid protein (ORF2) is a major structural protein of porcine circovirus type 2 (PCV2). A simple and reliable diagnostic method based on ORF2 protein immunoreactivity would serve as a valuable diagnostic method for detecting serum antibodies to PCV2 and monitoring PCV infection. Here, we reported an indirect enzyme-linked immunosorbent assay (I-ELISA) by using an antigenic domain …

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  • Detection of Classical swine fever virus infection by ...

    2019-8-25 · The ‘‘rope-in-a-bait’’ sampling method has been proven to detect CSFV infection in wild boars experimentally infected, although the animals had a very short viremic phase and virus excretion via saliva was very poor.13 A subsequent study9 provided evidence of simultaneous detection of Afri-can swine fever virus, CSFV, and FMDV in swine ...

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  • Rapid Detection of Porcine Circovirus Type 2 by TaqMan ...

    2021-3-15 · al, 2008). PCV2-infection is widespread and essentially all pig herds are infected with PCV2 in China. It is associated with distinct syndromes and diseases in swine, collectively known as porcine circovirus associated diseases (PCVAD), which include: • post-weaning multi-systemic wasting syndrome (PMWS) • PCV2-associated pneumonia as a part

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  • Phylogenetic analysis of Porcine Circovirus type 2 (PCV2 ...

    2015-1-30 · In this study, total DNA was isolated from 24 swine’s serum collected in 2013. ORF2 (Cap) of PCV2 was amplified and the PCR products were submitted for sequencing. The DNA sequences were analyzed and aligned using EditSeq and MegAlign version 7.1.0 (DNASTAR©). A phylogenetic tree was constructed using N-J method provided in MegAlign program.

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  • Fast diagnosis and quantification for porcine

    2010-4-1 · The postweaning multisystemic wasting syndrome, caused by the porcine circovirus type 2 (PCV-2), is a major disease that poses a significant threat to the global swine industry. The purpose of this study was to establish a real-time polymerase chain reaction (PCR) method for the quantification of PCV-2 and to enable the rapid differentiation of ...

    Get Price
  • Porcine circovirus 2 (PCV-2) genotype update and

    2018-12-6 · Porcine circovirus 2 (PCV-2) is one of the most widespread viral infections of swine, causing a remarkable economic impact because of direct losses and indirect costs for its control. As other ssDNA viruses, PCV-2 is characterized by a high evolutionary rate, leading to the emergence of a plethora of variants with different biological and epidemiological features.

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  • Sensitive detection of Porcine circovirus-2 by droplet ...

    2019-8-26 · pulmonasry infiltrates. PMWS infection was confirmed by immunohistochemical testing and PCR sequencing. The serum samples were stored at −70°C prior to extraction of total DNA using a commercial kit,a according to the manu-facturer’s instructions. Extracts were then stored at −20°C. Field isolates of Porcine circovirus-1 (PCV-1), porcine

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  • US Patent Application for VACCINES CONTAINING

    2018-4-12 · The invention also pertains to a method of prophylactically treating an animal against an infection with porcine circovirus type 2 (PCV2), an infection with Mycoplasma hyopneumoniae and an infection with PRRS virus by associated non-mixed administration of a first vaccine comprising non-replicating immunogen of porcine circo virus type 2 and ...

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  • Detection of porcine circoviruses in clinical specimens ...

    2018-8-27 · Detection of porcine circoviruses in clinical specimens using multiplex PCR in Hubei, central China 1 Keli Yang1,2, Zuwu Jiao1, Danna Zhou1,3, Rui Guo1, Zhengying Duan1, 2 Fangyan Yuan1 and Yongxiang Tian1,2 3 1Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan 430064, P. R. 4 China 5 2Key Laboratory of Prevention and Control Agents for …

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  • Prevalence of porcine circovirus type 3 in pigs in the ...

    2019-7-14 · Single infection with PCV3 accounted for only one-third of the samples, and majority were of co-infections, predominantly with PEDV (41.6%) but generally low with other swine viruses. Indirect ELISA using the PCV3 capsid protein as the coating antigen revealed an average sero-positive rate of 52.6% (40.8 to 60.8%) in 2345 serum samples from ...

    Get Price
  • Frontiers | Comparison of Multiplex Real-Time PCR

    2020-4-30 · Porcine circovirus type 2 (PCV2), the causative agent of porcine circovirus-associated diseases (PCVAD), poses a serious economic threat for the swine industry. Currently, PCV2 is classified into five major genotypes: PCV2a, PCV2b, PCV2c, PCV2d, and PCV2e. The aim of this study is to evaluate the performance of two commercially available methods, multiplex real-time PCR assay and …

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  • Diagnostic characteristics of circovirus infection in

    The aim of this study is to compare the results from the histopathology and the immunohistochemical method in the diagnostic of Porcine circovirus type 2 (PCV2) infection in pigs. The circovirus infection is a pig disease that is caused by a small, spherical, nonenveloped virus with a single stranded DNA genome which is spread throughout the pig industry worldwide.

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  • Porcine circovirus 2 (PCV-2) genotype update and

    2018-12-6 · Porcine circovirus 2 (PCV-2) is one of the most widespread viral infections of swine, causing a remarkable economic impact because of direct losses and indirect costs for its control. As other ssDNA viruses, PCV-2 is characterized by a high evolutionary rate, leading to the emergence of a plethora of variants with different biological and epidemiological features.

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  • Immunologic Features of Porcine Circovirus Type 2

    2019-9-9 · Clinical expression of porcine circovirus 2 (PCV-2) infection in swine may result in two distinct high mortality disease syndromes. In North America, postweaning multisystemic wasting syndrome (PMWS), while still sporadic in incidence, predominates.

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  • What has changed about porcine circovirus

    2020-4-3 · Images of major swine diseases. E-diagnostics. Pig disease diagnostic tool. Pig glossary. Definition for the most commonly used pig terms. Water medication calculator. Simulator that calculates the amount of drug to add to the water when using a flow dispenser.

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  • Detection of Classical swine fever virus infection by ...

    2019-8-25 · The ‘‘rope-in-a-bait’’ sampling method has been proven to detect CSFV infection in wild boars experimentally infected, although the animals had a very short viremic phase and virus excretion via saliva was very poor.13 A subsequent study9 provided evidence of simultaneous detection of Afri-can swine fever virus, CSFV, and FMDV in swine ...

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  • Porcine circovirus 2 (PCV2) population study in ...

    2020-10-20 · Porcine circovirus 2 (PCV2) is a single stranded DNA virus with one of the highest mutation rates among DNA viruses. This ability allows it to generate a cloud of mutants constantly providing new ...

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  • Multiple real time fluorescence quantifying PCR

    2007-3-9 · The method of the invention detects that the four virus are all in good linear relations at the same time, all ten times serial dilution points of constructed normal plasmid are all in one straight line, CT value and copy number are in good linear relation, and regression analysis shows that the related coefficient of the CT value and the copy ...

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  • Viruses | Free Full-Text | Porcine Circovirus 2

    Mitochondrial dynamics is essential for the maintenance of cell homeostasis. Previous studies have shown that porcine circovirus 2 (PCV2) infection decreases the mitochondrial membrane potential and causes the elevation of reactive oxygen species (ROS), which may ultimately lead to mitochondrial apoptosis. However, whether PCV2 induce mitophagy remains unknown.

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  • DOI: 10.1177/1040638713481471 using pen-based swine

    2010-1-2 · acute infection, hence sample collection success rates remain unchanged during acute infection with PRRSV5 and Influ-enza A virus (Millman S, Brooks R Jr, Zimmerman J, et al.: 2009, Role of behavior in non-invasive disease surveillance of swine influenza virus. J Anim Sci 87:E-Suppl:ii). Options for PRRSV surveillance using oral fluid speci-

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  • Detection of porcine circoviruses in clinical specimens ...

    2018-8-27 · Detection of porcine circoviruses in clinical specimens using multiplex PCR in Hubei, central China 1 Keli Yang1,2, Zuwu Jiao1, Danna Zhou1,3, Rui Guo1, Zhengying Duan1, 2 Fangyan Yuan1 and Yongxiang Tian1,2 3 1Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan 430064, P. R. 4 China 5 2Key Laboratory of Prevention and Control Agents for …

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