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yinzhijiedukeli for pseudorabies application

  • Establishment of a Fosmid Library for Pseudorabies

    Pseudorabies virus (PRV) is a member of Alphaherpesvirinae subfamily, its neurotropism and latency infection attract the attention of many scientists. PRV tagged with a fluorescent reporter gene as a tracker has been used to analyze neuronal circuits, including anterograde and retrograde. In …

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  • Label free detection of pseudorabies virus infection

    A new method for the detection of pseudorabies antibody in swine sera was developed by use of the indirect immunoperoxidase plaque staining test. The indirect immunoperoxidase plaque staining test demonstrated serologic responses in pigs that were subcutaneously infected with pseudorabies virus. ... Moutou F, Toma B, Fortier B. Application of ...

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  • Detection of antibody to pseudorabies virus in

    A novel time-resolved fluorescence blocking lateral flow immunoassay (TRF-BLFIA) was developed for on-site differential diagnosis of pseudorabies virus (PRV)-infected and vaccinated pigs using europium nanoparticles (EuNPs)-labeled virion antigens and high titer PRV gE monoclonal antibodies (PRV gE- …

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  • Differential diagnosis of PRV-infected versus

    Abstract. The performance of an indirect enzyme-linked immunosorbent assay and the standard serum neutralization test for detection of antibodies to pseudorabies virus in porcine field sera were compared, using 304 sera from pseudorabies-free pigs in Canada, and 1082 and 580 swine sera from the USA and England, respectively.

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  • Evaluation of an enzyme immunoassay for

    2020-7-14 · Establishment of a Fosmid Library for Pseudorabies Virus SC Strain and Application in Viral Neuronal Tracing.Front Microbiol .2020 Jun 11;11:1168. …

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  • Establishment of a Fosmid Library for Pseudorabies Virus ...

    2008-7-1 · A rapid, convenient and reliable pseudorabies virus (PRV) detection system was developed by using the loop-mediated isothermal amplification (LAMP) method. Six special primers were designed successfully based on the PRV DNA-binding protein (DBP) gene. The assay was optimized to amplify PRV DNA by incubation at 63 °C for 1 h.

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  • Loop-mediated isothermal amplification

    Relevance. Pseudorabies virus (PRV) is a member of the neurotropic viruses known as alphaherpesviruses. PRV is primarily a disease of swine which serve as a reservoir for the virus and the principal source of natural infection for a diverse range of secondary hosts, including cattle, sheep, goats, dogs, cats, and many feral species.

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  • Anti-Pseudorabies Virus antibody (ab3534) | Abcam

    Application. Available for Sale. United States Globally. Showing 1-1 of 1 result for 'Pseudorabies virus' Advanced Search. Structure Search. Relevance. Compare. 6D8 MB4. 6D8 MB4. Product Number ... Find Pseudorabies virus and related products for scientific research at MilliporeSigma

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  • Pseudorabies virus | Sigma-Aldrich

    2021-5-9 · Cyanovirin-N (CVN) is a cyanobacterial protein with potent neutralizing activity against enveloped virus. To achieve the economic and functional production of CVN, the CVN N-terminally fused with CL7(A mutant of the Colicin E7 Dnase) was utilized to improve the solubility and stability of CVN fusion protein (CL7-CVN). Additionally, to improve the detection limit of existing PRV diagnostic ...

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  • Supplemental Assay Method for Pseudorabies Virus

    2014-12-30 · Pseudorabies Virus Neutralizing Antibody ... A current Animal Use Application must be available prior to ordering the swine for the test. All constraints in the application must be followed. 3.2 Preparation of equipment/instrumentation Set the water bath at 36°± 2°C.

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  • Vaccines against pseudorabies virus (PrV) -

    2017-7-1 · Pseudorabies virus has been developed into a powerful vector system for integration and expression of foreign proteins. ... Application of DIVA vaccines and their companion diagnostic tests to foreign animal disease eradication. Anim. Health Res. Rev., 5 (2004), pp. 257-262.

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  • Dot-enzyme immunoassay for visual detection of

    A modified solid-phase enzyme immunoassay (EIA) is described for the visual detection of anti-pseudorabies virus (anti-PRV) antibody in porcine serum. Dots of PRV antigens were adsorbed to nitrocellulose paper (hence the name dot-EIA), and the remaining nonspecifically reactive sites were blocked with bovine serum albumin or skim milk powder.

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  • Loop-mediated isothermal amplification

    2008-7-1 · A rapid, convenient and reliable pseudorabies virus (PRV) detection system was developed by using the loop-mediated isothermal amplification (LAMP) method. Six special primers were designed successfully based on the PRV DNA-binding protein (DBP) gene. The assay was optimized to amplify PRV DNA by incubation at 63 °C for 1 h.

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  • CN107860921A - Pseudorabies virus antibody

    The invention discloses a kind of Pseudorabies virus antibody Quantitative detection card and application method, including detection card shell and the test strips being assemblied in detection card shell, the test strips include the plastic bottom board with pressure sensitive adhesive, paste sample pad successively on bottom plate, labeling pad, nitrocellulose filter and blotting paper, the ...

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  • Pseudorabies virus | Sigma-Aldrich

    Application. Available for Sale. United States Globally. Showing 1-1 of 1 result for 'Pseudorabies virus' Advanced Search. Structure Search. Relevance. Compare. 6D8 MB4. 6D8 MB4. Product Number ... Find Pseudorabies virus and related products for scientific research at MilliporeSigma

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  • PORCINE PSEUDORABIES VIRUS, VACCINE

    PORCINE PSEUDORABIES VIRUS, VACCINE COMPOSITION AND PREPARATION METHOD AND USE THEREOF . European Patent EP2985288 . Kind Code: B1 . Abstract: Provided in the present invention is a vaccine composition comprising an immune amount of attenuated live vaccine, inactivated vaccine, subunit vaccine, synthetic vaccine, or genetically engineered ...

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    308 Permanent Redirect - OIE ... nginx

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  • Enhancing expression of the pseudorabies virus ...

    ORIGINAL ARTICLE Enhancing expression of the pseudorabies virus glycoprotein E in yeast and its application in an indirect sandwich ELISA C.-Y. Wu1, C.-W. Wu2, C.-M. Liao3, M.-S. Chien4 and C. Huang1 1 Graduate Institute of Microbiology and Public Health, College of Veterinary Medicine, National Chung Hsing University, Taichung, Taiwan

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  • Detection of antibody to pseudorabies virus in swine

    A new method for the detection of pseudorabies antibody in swine sera was developed by use of the indirect immunoperoxidase plaque staining test. The indirect immunoperoxidase plaque staining test demonstrated serologic responses in pigs that were subcutaneously infected with pseudorabies virus. ... Moutou F, Toma B, Fortier B. Application of ...

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  • Establishment of a Fosmid Library for Pseudorabies

    2020-6-11 · The first fosmid library for pseudorabies variant strain PRV TJ has been reported in 2018 by our group (Zhou et al., 2018). In this study, a fosmid library for pseudorabies strain PRV SC was established using the same method. In this study, we got 200 fosmid DNAs that cover the whole genome of …

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  • Human Endophthalmitis Caused By Pseudorabies

    1A8 was deposited to the DSHB by Enquist, L. Lyman, M. (DSHB Hybridoma Product 1A8) Storage and Handling Recommendations. Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended.

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  • Us9 -pseudorabies virus Antibody (1A8) - DSHB

    2020-7-2 · Pseudorabies virus (PRV) is a major pathogen in pig husbandry and is also a risk to human well‐being. Pigs with latent PRV infection carry the virus lifelong, and it can be activated under conducive conditions. This poses a very important challenge to the control of the virus and may even prevent its elimination. To investigate latent ...

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  • Latent pseudorabies virus infection in medulla

    2017-11-9 · A novel gE-deleted pseudorabies virus (PRV) provides rapid and complete protection from lethal challenge with the PRV variant emerging in Bartha-K61-vaccinated swine population in China. Vaccine. 2014, 32:3379-3385. 17) Sun Y, Tian DY, Li S, Meng QL

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  • New Technology for Pseudorabies PRV Vaccine

    Pseudorabies (Aujeszky's disease) is a viral disease caused by pseudorabies virus (PRV). PRV is mainly spread via direct contact, but may also transmit by air, water and contaminated fomites. Outbreaks of PRV in pigs are difficult to control, causing catastrophic economic losses in the swine industry.

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  • Enhancing expression of the pseudorabies virus ...

    2017-7-8 · Introduction. Pseudorabies virus (PRV) is an alphaherpesvirus that causes pseudorabies (also known as Aujeszky's disease) in swine, which causes severe economic losses worldwide (Mettenleiter 2000).The disease is characterized by an acute and often fatal infection in piglets, retarded growth in growing pigs and abortion in pregnant sows (Nauwynck 1997).

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  • Molecular Biology of Pseudorabies Virus: Impact on ...

    SUMMARY Pseudorabies virus (PRV) is a herpesvirus of swine, a member of the Alphaherpesvirinae subfamily, and the etiological agent of Aujeszky's disease. This review describes the contributions of PRV research to herpesvirus biology, neurobiology, and viral pathogenesis by focusing on (i) the molecular biology of PRV, (ii) model systems to study PRV pathogenesis and neurovirulence, (iii) PRV ...

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  • Application of in situ viral nucleic acid hybridization to ...

    2020-10-13 · Application of in situ viral nucleic acid hybridization to the study of Aujeszky's disease (pseudorabies) in calves Harold Antonio McAllister Iowa State University Follow this and additional works at:https://lib.dr.iastate.edu/rtd Part of theAnimal Sciences Commons, and …

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  • APPLICATION FOR REGISTERED SWINE EXHIBITION/SALE

    2021-6-9 · certificate of veterinary inspection, official identification and a negative pseudorabies test within 30 days of the exhibition. THIS INFORMATION PROVIDED ON THIS APPLICATION IS TRUE AND ACCURATE UNDER PENALTY Signature of Applicant, Title, and E -Mail Address Email form to : [email protected]

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  • Gene Cloning and Bioinformatics Analysis of

    The glycoprotein H(gH) gene homologue of pseudorabies virus wild strain SL(PRV-SL) was cloned by degenerate polymerase chain reaction (PCR) from PRV infected vero cells. Bioinformatics analysis was performed to predict the characteristics of gH, the results indicated that gH gene encoded a polypeptide, molecular mass of 71.95kda and comprising 687 amino acids.

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  • Review Article An Overview of Live Attenuated

    2019-7-31 · Review Article An Overview of Live Attenuated Recombinant Pseudorabies Viruses for Use as Novel Vaccines BoDong, 1 DanteS.Zarlenga, 2 andXiaofengRen 1 Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University,

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  • For Health Requirement Information

    2021-6-3 · pseudorabies testing requirement. 2. Swine originating from a Pseudorabies Stage III state shall be negative to an official test for pseudorabies conducted within thirty (30) days prior to entry or originate immediately and directly from a qualified pseudorabies negative herd. 3.

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  • SVANOVIR® PRV gB-Ab Controlling

    2021-3-14 · PERFORMANCE ChARACTERISTICS SVANOVIR® PRV gB-Ab The excellent performance of SVANOVIR® PRV gB-Ab has been demonstrated by testing a comprehensive number of serum sam-

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  • Human Endophthalmitis Caused By Pseudorabies Virus ...

    2021-7-13 · pseudorabies virus from patient with human endophthalmitis, China, 2017. A) Sequencing of Suid herpesvirus 1 (pseudorabies virus) yielded a total coverage of 84%. B) Evolutionary relationships of taxa. Phylogenetic analysis disclosed a close connection between the isolate from the patient (boldface) and 4 other Suid herpesvirus 1 strains. Scale bar

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  • Program Standards - USDA

    2003-11-1 · 2. All swine must originate from a State that has achieved Pseudorabies Eradication Program status of Stage III, IV, or V; or 3. All swine must originate in a pseudorabies-monitored feeder-pig herd; or 4. All swine are found negative to an official pseudorabies test conducted 30 days or less prior to presentation at the market.

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  • Pseudorabies - PRV | Iowa State University

    2021-7-16 · Pseudorabies virus (PRV) is an alphavirus in a subfamily of Herpesviridae. Isolates vary in virulence and pathogenicity. Like many other herpesviruses, PRV often persists in a latent state in recovered animals. The virus is only moderately resistant outside the host, survival depending largely on environmental factors.

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  • Dot-enzyme immunoassay for visual detection of

    2021-2-2 · A modified solid-phase enzyme immunoassay (EIA) is described for the visual detection of anti-pseudorabies virus (anti-PRV) antibody in porcine serum. Dots of PRV antigens were adsorbed to nitrocellulose paper (hence the name dot-EIA), and the remaining nonspecifically reactive sites were blocked with bovine serum albumin or skim milk powder. After immersion in test serum, bound …

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  • Establishment and Application of PCR in Porcine ...

    To differentiate gE gene-deleted vaccine and porcine Pseudorabies virus(PRV),a PCR method was established after selecting the optimal amplification conditions based on the sequences of gE and gB of the PRV genome.Through this,two fragments of 400 bp for gE gene and 582 bp for gB gene were simultaneously amplified from pseudorabies virus virulent strains DNA,only a 582 bp fragment for gB …

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  • Development and application of a real-time

    This study was conducted to establish a rapid and specific quantitative PCR assay for detecting Pseudorabies virus (PRV). A pair of primers and a Taq Man probe were designed according to conserved gE gene sequences of (PRV). PCR conditions were optimized to establish a Taq Man-based real-time PCR assay for the detection of PRV. The assay was tested on samples from cell culture, suspected …

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  • Viruses | Free Full-Text | Recombinant Pseudorabies

    Owing to viral evolution and recombination, emerging pseudorabies virus (PRV) strains have caused unprecedented outbreaks in swine farms even when the pigs were previously vaccinated, which might indicate that traditional vaccines were unable to provide effective protection. The development of safe and efficacious vaccines presents prospects to minimize the clinical signs and eventually ...

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  • Pseudorabies virus expressing enhanced green

    Pseudorabies virus expressing enhanced green fluorescent protein: A tool for in vitro electrophysiological analysis of transsynaptically labeled neurons in identified central nervous system circuits Bret N. Smith*†, Bruce W. Banfield‡§, Cynthia A. Smeraski*, Christine L. Wilcox¶, F. Edward Dudek*, Lynn W. Enquist‡, and Gary E. Pickard*i

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  • UNIVERSITI PUTRA MALAYSIA ESTABLISHMENT OF A

    2019-7-10 · BASED VIRAL ASSAY FOR PSEUDORABIES VIRUS INFECTION By PARISA HONARI November 2011 Chairman: Assoc. Prof. Zeenathul Nazariah Allaudin, PhD Faculty: Veterinary Medicine Studies were conducted to establish a viral based biochip using Surface Plasmon Resonance (SPR) technology for detection of Pseudorabies virus (PrV.

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  • Effects of physical and chemical factors on

    2020-9-25 · Pseudorabies (PR) is latent and can persist in infected sows for a long time, and thus, convalescent sows can carry the virus throughout life, causing severe economic losses to farmers and posing a tremendous challenge to PR prevention and control. Here, to investigate the biological characteristics of pseudorabies virus (PRV), a variety of physical and chemical factors were analyzed …

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  • Studies on Neuronal Tracing with Pseudorabies Virus ...

    With its abilities of trans-synaptic tracing and self-replication and wide host range,pseudorabies virus(PRV)has been applied in the field of neuroanatomy since the 1970s.Four decades of PRV application have made many advances in researches on neuronal tracing with PRV.Mechanism studies focused on investigating infection of primary neurons and tracing direction in secondary neurons,while ...

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  • Porcine pseudorabies virus (PRV) variant PRV-ZJ01

    The invention relates to the technical field of porcine pseudorabies viruses (PRVs) and in particular relates to a porcine PRV variant PRV-ZJ01 with collection number of CGMCCNo.8170 and an application of the porcine PRV variant PRV-ZJ01 in preparation of vaccines. The porcine PRV variant PRV-ZJ01 has the beneficial effects that a water-soluble inactivated vaccine is prepared by adopting a PRV ...

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  • Location of density gradient ultracentrifugation layers

    During the process of virus density gradient ultracentrifugation purification, the centrifugal zone formed after ultracentrifugation can be located by the scattering intensity of light of different-sized particles, also known as the Tyndall effect. The pseudorabies virus (PRV) was selected and taken as the research object and discontinuous gradient sucrose as the medium, the particles in the...

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  • Ivermectin, a new candidate therapeutic against SARS

    2017-1-1 · 《药物分析杂志》是中国科学技术协会主管,中国药学会主办,中国食品药品检定研究院承办,药物分析杂志编辑委员会编辑出版,国内外公开发行的专业性学术期刊。《药物分析杂志》是我国药检领域首批学科带头人创办的学术期刊,由始创于1951年的《药检工作通讯》发展而来,1981年更名为 ...

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