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xielian for epidemic diarrhea using method

  • Visual detection of porcine epidemic diarrhea virus

    Visual detection of porcine epidemic diarrhea virus using a novel reverse transcription polymerase spiral reaction method. These results indicate that this RT-PSR assay is capable of accurately detecting PEDV, and has the advantages of high specificity and sensitivity for the detection of PEDV.

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  • Novel isolation method of porcine epidemic diarrhea

    Novel isolation method of porcine epidemic diarrhea virus using suspension vero cells and immunogenicity analysis. J Clin Microbiol. 2020 Nov 11;JCM.02156-20. doi: 10.1128/JCM.02156-20.

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  • Inactivation of porcine epidemic diarrhea virus using ...

    2016-12-1 · Porcine epidemic diarrhea virus (PEDV) is a very contagious pathogen that has become a major economic burden for U.S. swine producers since its initial North American detection in May 2013. This coronavirus is estimated to have killed over 7 million pigs in 2013 alone with significant additional swine morbidity and mortality in subsequent years ( Chen et al., 2014 , Cima, 2013 , Cima, 2014 ).

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  • Porcine epidemic diarrhea virus: Viral RNA detection

    Since 2014, porcine epidemic diarrhea virus (PEDV) has reemerged in Europe. RT-PCR methods have been described for the detection of PEDV, but none have been validated according to a norm. In this study we described the development and validation of a SYBR™ Green one-step RT-qPCR according to the French norm NF U47-600, for the detection and quantification of PEDV viral RNA.

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  • Visual detection of porcine epidemic diarrhea virus

    2019-4-15 · Visual detection of porcine epidemic diarrhea virus using a novel reverse transcription polymerase spiral reaction method. Wang X (1), Xu X (1), Hu W (1), Zuo K (2), Li Z (3), Kan Y (1), Yao L (4), Ji J (5), Bi Y (3). Author information: (1)Henan Provincial Engineering Laboratory of Insects Bio-reactor, China-UK-NYNU-RRes Joint Laboratory of Insect ...

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  • Porcine epidemic diarrhea virus: An overview of

    2016-12-2 · Porcine epidemic diarrhea virus (PEDV) antigens can be detected in feces using antigen capture ELISAs (Callebaut et al., 1982, Carvajal et al., 1995, Rodák et al., 2005). PEDV antigen capture or sandwich ELISAs are based on the use of a pair of PEDV-specific antibodies to capture and detect virus antigens onto a solid phase ( Aydin, 2015 , Callebaut et al., 1982 , Carvajal et al., 1995 , Rodák …

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  • A novel biotinylated nanobody-based blocking ELISA

    Background: Porcine epidemic diarrhea virus (PEDV), which is characterized by severe watery diarrhea, vomiting, dehydration and a high mortality rate in piglets, leads to enormous economic losses to the pork industry and remains a large challenge worldwide. Thus, a rapid and reliable method is required for epidemiological investigations and to evaluate the effect of immunization.

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  • Effectiveness of composting as a biosecure disposal

    Background: Porcine epidemic diarrhea virus (PEDV) is an enteric disease of swine that has emerged as a worldwide threat to swine herd health and production. Substantial research has been conducted to assess viability of the virus on surfaces of vehicles and equipment, in feed and water, and on production building surfaces, but little is known about the persistence in PEDV-infected carcasses and effective …

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  • Phylogenetic analysis of porcine epidemic diarrhea

    2012-11-15 · Porcine epidemic diarrhea virus (PEDV) is the causative agent of porcine epidemic diarrhea (PED), which is characterized by severe diarrhea, dehydration and high mortality in the affected pigs. Recently, clinical outbreaks of diarrhea in suckling piglets emerged in pig-producing areas of China.

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  • A sensitive duplex nanoparticle-assisted PCR assay for ...

    2016-11-4 · In this study, a novel duplex nanoparticle-assisted polymerase chain reaction (nanoPCR) assay was developed to detect porcine epidemic diarrhea virus (PEDV) and porcine transmissible gastroenteritis virus (TGEV). Two pairs of primers were designed based on the conserved region within the N gene of PEDV and TGEV.

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  • Inactivation of porcine epidemic diarrhea virus using ...

    2016-12-1 · Porcine epidemic diarrhea virus (PEDV) is a very contagious swine pathogen that spreads easily via the fecal-oral route, notably from contaminated fomites. The present study investigated heated water as a method for rapid thermal inactivation of PEDV. Cell-culture adapted PEDV was treated with water at varying temperatures and viral titers were ...

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  • Visual detection of porcine epidemic diarrhea virus

    2019-4-15 · Porcine epidemic diarrhea virus (PEDV) is a major etiological agent of porcine epidemic diarrhea around the world. Point-of-care testing in the field is lacking owing to the requirement for a simple, robust field applicable test that does not require professional laboratory equipment. The aim of this study was to establish a novel reverse transcription polymerase spiral reaction (RT-PSR) assay ...

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  • Visual detection of porcine epidemic diarrhea virus

    2019-4-15 · 1. BMC Vet Res. 2019 Apr 15;15(1):116. doi: 10.1186/s12917-019-1851-7. Visual detection of porcine epidemic diarrhea virus using a novel reverse transcription polymerase spiral reaction method.

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  • Porcine Epidemic Diarrhea (PED) - USDA

    2018-7-16 · Porcine Epidemic Diarrhea (PED) OVERVIEW. The porcine epidemic diarrhea (PED) virus was first recognized in the United Kingdom in 1971 and had spread throughout much of Europe and Asia by 2013. The United States Department of Agriculture’s (USDA) National Veterinary Services Laboratories (NVSL)

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  • Forecasting incidence of infectious diarrhea using

    2020-3-14 · Infectious diarrhea can lead to a considerable global disease burden. Thus, the accurate prediction of an infectious diarrhea epidemic is crucial for public health authorities. This study was aimed at developing an optimal random forest (RF) model, considering meteorological factors used to predict an incidence of infectious diarrhea in Jiangsu Province, China.

    Get Price
  • Phylogenetic analysis of porcine epidemic diarrhea

    2012-11-15 · Porcine epidemic diarrhea virus (PEDV) is the causative agent of porcine epidemic diarrhea (PED), which is characterized by severe diarrhea, dehydration and high mortality in the affected pigs. Recently, clinical outbreaks of diarrhea in suckling piglets emerged in pig-producing areas of China. In this study, molecular detection of PEDV was conducted using RT-PCR (targeting the M gene) on ...

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  • Survival of porcine epidemic diarrhea virus (PEDV) in ...

    2017-9-19 · Infection with Porcine Epidemic Diarrhea Virus (PEDV) causes vomiting, diarrhea, and dehydration in young pigs. The virus made its first appearance in the U.S. in 2013, where it caused substantial neonatal mortality and economic losses in the U.S. pork industry. Based on outbreak investigations, it is hypothesized that the virus could be transmitted through contaminated feed or …

    Get Price
  • Whole-Genome Sequencing of Porcine Epidemic

    2016-3-16 · Porcine epidemic diarrhea virus (PEDV) belongs to the genus Alphacoronavirus of the family Coronaviridae. PEDV was identified as an emerging pathogen in US pig populations in 2013. Since then, this virus has been detected in at least 31 states in the USA and has caused significant economic loss to the swine industry.

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  • A descriptive survey of porcine epidemic diarrhea in

    2020-10-4 · Porcine epidemic diarrhea (PED) virus (PEDV) is a globally emerging and re-emerging epizootic swine virus that causes massive economic losses in the swine industry, with high mortality in piglets. In Vietnam, PED first emerged in 2009 and has now developed to an endemic stage. This is the first cross-sectional survey performed to evaluate the proportion of PEDV-positive swine farms in …

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  • A sensitive duplex nanoparticle-assisted PCR assay for ...

    2016-11-4 · In this study, a novel duplex nanoparticle-assisted polymerase chain reaction (nanoPCR) assay was developed to detect porcine epidemic diarrhea virus (PEDV) and porcine transmissible gastroenteritis virus (TGEV). Two pairs of primers were designed based on the conserved region within the N gene of PEDV and TGEV. In a screening of 114 clinical samples from four provinces in China …

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  • Laboratory Methods for the Diagnosis of Epidemic

    2010-10-23 · Epidemic Diarrhea The two most common types of epidemic diarrhea in developing countries are watery diarrhea caused by Vibrio cholerae serogroup O1 and bloody. diarrhea caused by Shigella dysenteriae serotype 1 (Sd1). This chapter presents an overview of these and other organisms that cause epidemic dysentery and cholera.

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  • Porcine Epidemic Diarrhea (PED) - USDA

    2018-7-16 · Porcine Epidemic Diarrhea (PED) OVERVIEW. The porcine epidemic diarrhea (PED) virus was first recognized in the United Kingdom in 1971 and had spread throughout much of Europe and Asia by 2013. The United States Department of Agriculture’s (USDA) National Veterinary Services Laboratories (NVSL)

    Get Price
  • Detection of porcine epidemic diarrhea virus by ...

    Detection of porcine epidemic diarrhea virus by immunohistochemistry with recombinant antibody produced in phages. Lee HM(1), Lee BJ, Tae JH, Kweon CH, Lee YS, Park JH. Author information: (1)Department of Veterinary Public Health, College of Veterinary …

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  • The construction of recombinant Lactobacillus casei ...

    2021-5-4 · Porcine epidemic diarrhea (PED) is a contagious intestinal disease caused by porcine epidemic diarrhea virus (PEDV) characterized by vomiting, diarrhea, anorexia, and dehydration, which have caused huge economic losses around the world. At present, vaccine immunity is still the most effective method to control the spread of PED. In this study, we have constructed a novel recombinant …

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  • An evaluation of contaminated complete feed as a

    2014-8-15 · Epidemic Diarrhea was diagnosed in 3 breeding herds following acute outbreaks of anorexia, diarrhea and vomiting in isolated groups of sows. These herds were part of an organized system of commercial pork produc-tion; Farm A (4973 sows) was located in NW Iowa, while Farms B and C, 3390 sows and 3016 sows respect-ively, were located in SW Minnesota.

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  • Systemic and intestinal porcine epidemic diarrhea

    2017-9-19 · Infection with Porcine Epidemic Diarrhea Virus (PEDV) causes vomiting, diarrhea, and dehydration in young pigs. The virus made its first appearance in the U.S. in 2013, where it caused substantial neonatal mortality and economic losses in the U.S. pork industry. Based on outbreak investigations, it is hypothesized that the virus could be transmitted through contaminated feed or …

    Get Price
  • Survival of porcine epidemic diarrhea virus (PEDV)

    economic losses worldwide. Porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV) are the main pathogens responsible for piglet viral diarrhea. However, currently there is no specific drug available for the effective treatment of viral diarrhea. Therefore, it is necessary to seek an effective method to

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  • Construction of multiple shRNA vectors targeting PEDV

    2021-3-22 · Porcine epidemic diarrhea (PED), caused by PEDV, is an intestinal infectious disease characterized by vomiting, anorexia, watery diarrhea, and dehydration 9, …

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  • Puerarin enhances intestinal function in piglets

    2017-9-21 · Porcine Deltacoronavirus (PDCoV) is a newly emerged enteropathogenic coronavirus that causes diarrhea and mortality in neonatal piglets. PDCoV has spread to many countries around the world, leading to significant economic losses in the pork industry. Therefore, a rapid and sensitive method for detection of PDCoV in clinical samples is urgently needed.

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  • Forecasting incidence of infectious diarrhea using

    2020-3-14 · Infectious diarrhea can lead to a considerable global disease burden. Thus, the accurate prediction of an infectious diarrhea epidemic is crucial for public health authorities. This study was aimed at developing an optimal random forest (RF) model, considering meteorological factors used to predict an incidence of infectious diarrhea in Jiangsu Province, China.

    Get Price
  • Molecular characterization and phylogenetic analysis

    2017-10-10 · Large-scale outbreaks of porcine epidemic diarrhea (PED) have re-emerged in China in recent years. However, little is known about the genetic diversity and molecular epidemiology of field strains of PED virus (PEDV) in China in 2016–2017. To address this issue, in this study, 116 diarrhea samples were collected from pig farms in 6 Chinese provinces in 2016–2017 and were detected using …

    Get Price
  • Detection and phylogenetic analyses of spike genes in ...

    2020-10-4 · Porcine epidemic diarrhea (PED) virus (PEDV) is a globally emerging and re-emerging epizootic swine virus that causes massive economic losses in the swine industry, with high mortality in piglets. In Vietnam, PED first emerged in 2009 and has now developed to an endemic stage. This is the first cross-sectional survey performed to evaluate the proportion of PEDV-positive swine farms in …

    Get Price
  • A descriptive survey of porcine epidemic diarrhea in

    BMC Veterinary Research (2019-01-01) . A new immunochromatographic assay for on-site detection of porcine epidemic diarrhea virus based on monoclonal antibodies prepared by using cell surface fluorescence immunosorbent assay

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  • A new immunochromatographic assay for on-site

    2021-5-31 · RT-PCR method is a specific and sensitive diagnostic tool for the detection of PEDV from suspected tissue or fecal sample [17]. EM is used to detect viral particles in fecal material with diarrhea, but EM is not a sensitive technique [18]. Although, SNT is time-consuming

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  • The Carboxy Terminal Region on Spike Protein of Porcine ...

    2011-2-1 · Porcine epidemic diarrhea (PED) is an infectious enteric disease characterized by acute enteritis and diarrhea in pigs, and the infection is more severe in neonates [].At present, PED has been a major concern in the swine industry, particularly, in the Asia and Europe, resulting in large economic losses [2–4].The causative agent of PED is porcine epidemic diarrhea virus (PEDV), an enveloped ...

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  • Development of reverse transcription loop-mediated ...

    2015-4-16 · Porcine deltacoronavirus (PDCoV) is a novel coronavirus that causes diarrhea in nursing piglets. Following its first detection in the United States in February 2014, additional PDCoV strains have been identified in the United States and Canada. Currently, no treatments or vaccines for PDCoV are available. In this study, U.S. PDCoV strain OH-FD22 from intestinal contents of a diarrheic pig from ...

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  • Isolation and Characterization of Porcine

    2021-3-7 · Rapid detection of porcine deltacoronavirus and porcine epidemic diarrhea virus using the duplex recombinase polymerase amplification Journal of Virological Methods ( IF 1.786) Pub Date : 2021 Gen Li, Miaoli Wu, Jinhui Li, Weiyou Cai, Yongsheng Xie, Guangbing Si, Li …

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  • Specific, simple and rapid detection of porcine

    2011-3-18 · Porcine circovirus type 2 (PCV2) is the causative agent of postweaning multisystemic wasting syndrome (PMWS), and porcine dermatitis and nephropathy syndrome (PDNS). It has caused heavy losses in global agriculture in recent decades. Rapid detection of PCV2 is very important for the effective prophylaxis and treatment of PMWS. A loop-mediated isothermal amplification (LAMP) assay …

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  • Detection of porcine epidemic diarrhea virus by ...

    Detection of porcine epidemic diarrhea virus by immunohistochemistry with recombinant antibody produced in phages. Lee HM(1), Lee BJ, Tae JH, Kweon CH, Lee YS, Park JH. Author information: (1)Department of Veterinary Public Health, College of Veterinary …

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  • Systemic and intestinal porcine epidemic diarrhea

    2014-12-20 · Porcine epidemic diarrhea virus (PEDV) was detected in May 2013 for the first time in U.S. swine and has since caused significant economic loss. Obtaining a U.S. PEDV isolate that can grow efficiently in cell culture is critical for investigating pathogenesis and developing diagnostic assays and for vaccine development. An additional objective was to determine which gene(s) of PEDV is most ...

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  • Isolation and Characterization of Porcine Epidemic ...

    2021-3-22 · Porcine epidemic diarrhea (PED), caused by PEDV, is an intestinal infectious disease characterized by vomiting, anorexia, watery diarrhea, and dehydration 9, …

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  • Puerarin enhances intestinal function in piglets

    2017-10-10 · Large-scale outbreaks of porcine epidemic diarrhea (PED) have re-emerged in China in recent years. However, little is known about the genetic diversity and molecular epidemiology of field strains of PED virus (PEDV) in China in 2016–2017. To address this issue, in this study, 116 diarrhea samples were collected from pig farms in 6 Chinese provinces in 2016–2017 and were detected using …

    Get Price
  • Detection and phylogenetic analyses of spike genes in ...

    2018-5-29 · Porcine epidemic diarrhoea (PED) is an emerging disease in pigs that causes massive economic losses in the swine industry, with high mortality in suckling piglets. Early identification of PED virus (PEDV)-infected herd through surveillance or monitoring strategies is necessary for mass control of PED. However, a common working diagnosis system involves identifying PEDV-infected animals ...

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  • Development of pooled testing system for porcine

    Porcine epidemic diarrhea, PED ELISA kit (GREEN ABIOTECHNOLOGY CO., LTD) Category: Pharmaceutical and Medical Devices Exhibitor: TUNGHAI UNIVERSITY Booth No: R1327 Characteristic . The kit is used to simultaneously detect the porcine epidemic dysentery virus (PEDV) in individual or mixed samples of pigs, such as feces, tissues, oral fluid and swabs.

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  • Porcine epidemic diarrhea, PED ELISA kit (GREEN ...

    Modelling the dynamics of direct and pathogens-induced dysentery diarrhoea epidemic with controls. Berhe HW(1), Makinde OD(2), Theuri DM(3). Author information: (1)a Pan African University Institute of Basic Sciences Technology and Innovation , Nairobi , Kenya. (2)b Faculty of Military Science , Stellenbosch University , Cape Town , South Africa.

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  • Modelling the dynamics of direct and pathogens

    2020-9-30 · Infectious diarrhea has high morbidity and mortality around the world. For this reason, diarrhea prediction has emerged as an important problem to prevent and control outbreaks. Numerous studies have built disease prediction models using large-scale data. However, these methods perform poorly on diarrhea data. To address this issue, this paper proposes a parsimonious model (PM), …

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  • Short-Term Infectious Diarrhea Prediction Using

    2019-3-19 · Porcine epidemic diarrhea virus (PEDV), a serious swine epidemic, has been rampant in Asia since the 1990s. Despite the widespread use of PEDV vaccines, the occurrence of PEDV variants requires the discovery of new substances that inhibit these viruses. During a search for PEDV inhibitory materials from natural sources, seven new sabphenosides (1–7) and a new flavonoid (8), as well as …

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  • Isolation and Characterization of Porcine Epidemic ...

    2014-12-20 · Porcine epidemic diarrhea virus (PEDV) was detected in May 2013 for the first time in U.S. swine and has since caused significant economic loss. Obtaining a U.S. PEDV isolate that can grow efficiently in cell culture is critical for investigating pathogenesis and developing diagnostic assays and for vaccine development. An additional objective was to determine which gene(s) of PEDV is most ...

    Get Price
  • Detection and phylogenetic analysis of porcine

    2016-11-25 · Porcine epidemic diarrhea (PED) has increased in severity in China since 2010. To investigate further the infectivity, genetic diversity and molecular epidemiology of its causative agent, the porcine epidemic diarrhea virus (PEDV), we assessed 129 clinical samples, which were the intestinal tissue of piglets with severe diarrhea, from 17 cities in central China.

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  • Tight Junction Protein Occludin Is a Porcine Epidemic ...

    Porcine epidemic diarrhea virus (PEDV), the causative agent of porcine epidemic diarrhea, has caused huge economic losses in pig-producing countries. Although PEDV was long believed to replicate in the intestinal epithelium by using aminopeptidase N as a receptor, the mechanisms of PEDV infection are not fully characterized. In this study, we found that PEDV infection of epithelial cells ...

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  • Tomatidine inhibits porcine epidemic diarrhea virus ...

    2021-5-4 · Porcine epidemic diarrhea (PED) is a contagious intestinal disease caused by porcine epidemic diarrhea virus (PEDV) characterized by vomiting, diarrhea, anorexia, and dehydration, which have caused huge economic losses around the world. At present, vaccine immunity is still the most effective method to control the spread of PED. In this study, we have constructed a novel recombinant …

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  • The construction of recombinant Lactobacillus casei ...

    2019-11-13 · Porcine epidemic diarrhea virus (PEDV) is a highly virulent coronavirus that causes severe enteric disease in pigs ().PEDV infection manifests itself as watery diarrhea and vomiting and leads to dangerous dehydration that is often fatal, particularly in neonatal piglets (2, 3).PEDV was first isolated in the United Kingdom in the early 1970s, where it was initially believed to be a new strain ...

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  • The 3.1-Angstrom Cryo-electron Microscopy Structure

    2019-1-18 · Porcine epidemic diarrhea virus (PEDV) is a highly effective pathogen that can cause death of new-born piglet, resulting in big economical loss in pig farming industry. For rapid detection of PEDV, a new immunochromatographic assay (ICA) based on monoclonal antibodies (mAbs) was developed in this study. The mAbs were prepared by using PEDV positive hybridoma cells that were selected by using ...

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  • A new immunochromatographic assay for on-site

    2019-7-16 · Diarrhea, caused by porcine epidemic diarrhea virus (PEDV), is a catastrophic gastrointestinal disease among suckling piglets, with high infectivity, morbidity, and mortality, causing huge economic losses to the pig industry. In the present study, we investigated the different microbiota from the cecal mucosa and cecal contents between healthy and PEDV-infected piglets.

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  • Changes in cecal microbiota community of suckling

    2019-3-19 · Porcine epidemic diarrhea virus (PEDV), a serious swine epidemic, has been rampant in Asia since the 1990s. Despite the widespread use of PEDV vaccines, the occurrence of PEDV variants requires the discovery of new substances that inhibit these viruses. During a search for PEDV inhibitory materials from natural sources, seven new sabphenosides (1–7) and a new flavonoid (8), as well as …

    Get Price
  • Prenylated Phenolic Compounds from the Leaves of

    2001-11-1 · Transmissible gastroenteritis (TGE) and porcine epidemic diarrhea (PED) are highly contagious enteric diseases of piglets. The clinical signs of these diseases are very similar and include watery, yellowish diarrhea. Thus, the effective differential detection …

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  • Porcine Epidemic Diarrhea (PED) - USDA

    2018-7-16 · Porcine Epidemic Diarrhea (PED) OVERVIEW. The porcine epidemic diarrhea (PED) virus was first recognized in the United Kingdom in 1971 and had spread throughout much of Europe and Asia by 2013. The United States Department of Agriculture’s (USDA) National Veterinary Services Laboratories (NVSL)

    Get Price
  • Porcine epidemic diarrhea virus (PEDV): An update on ...

    2020-6-2 · Porcine epidemic diarrhea virus (PEDV), a member of the genus Alphacoronavirus in the family Coronaviridae, causes acute diarrhea and/or vomiting, dehydration and high mortality in neonatal piglets.Two different genogroups of PEDV, S INDEL [PEDV variant containing multiple deletions and insertions in the S1 subunit of the spike (S) protein, G1b] and non-S INDEL (G2b) strains were …

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  • Analysis of Cholera Epidemic Controlling Using ...

    The cholera epidemic is a fatal waterborne disease causing diarrhea, dehydration, and vomiting in an individual [1, 2]. It is caused by a bacterium called Vibrio cholerae . Cholera is transmitted through ingesting contaminated drinks and food, contact with cholera patient’s feces, and touching vomit and corpse killed by the bacterium without ...

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  • Their Antiviral Activities against Porcine Epidemic ...

    2019-7-31 · Their Antiviral Activities against Porcine Epidemic Diarrhea Virus ... cell viability was measured using the MTT method. IC50 values were calculated using SigmaPlot 10.0 software. Figure S63: The inhibitory effects of compounds 7, 15, and 16 (20 μM) on the expression

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  • Laboratory Processing of Fecal Specimens

    of unknown origin. Only a few pathogens cause epidemic diarrhea, although many cause sporadic diarrhea.S. dysenteriae serotype 1 and V. cholerae are the two etiologic agents responsible for most epidemic diarrhea in the developing world, contributing substantially to the burden of morbidity and mortality.S. Typ hi, the

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  • Molecular characterization and phylogenetic analysis of ...

    2017-3-9 · Porcine epidemic diarrhea (PED) is a disease that is considered to be devastating for pig farmers with a rapid spread of infection (Wang et al., 2013; Alonso et al., 2014; Jung and Saif, 2015). The clinical symptoms of PED include severe enteritis, vomiting, and watery diarrhea, with high infectivity and lethality in piglets.

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  • Steps to Creating a Basic Epidemic Curve Using Microsoft ...

    2011-9-13 · Steps to Creating a Basic Epidemic Curve Using Microsoft Excel 20 07 7 Step 6a – Select the Insert ribbon, then press and hold the Columns button to bring up the Columns submenu. Under 2-D column select the middle button for the two-dimensional chart option. [Note: Is not necessary to use 3-D chart options for epidemic curves as it makes reading

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  • Characterization and pathogenicity of Vero cell

    2019-10-28 · Biological characteristics of porcine epidemic diarrhea virus strains after 10, 64, or 120 passages. a Cytopathic effects of the three strains 12 hpi at an MOI of 0.01.b Immunofluorescence detection results for the P10, P64, and P120 strains in Vero cells infected at an MOI of 0.01 at 12 hpi.c Growth kinetics of the P10, P64, and P120 strains in Vero cells at an MOI of 0.01.

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  • Multiplex Reverse Transcription-PCR for Rapid

    2006-5-1 · Jung, K, Chae, C: 2005, RT-PCR-based dot blot hybridization for the detection and differentiation between porcine epidemic diarrhea virus and transmissible gastroenteritis virus in fecal samples using a non-radioactive digoxigenin cDNA probe.

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  • Forecasting incidence of infectious diarrhea using

    2020-3-14 · Infectious diarrhea can lead to a considerable global disease burden. Thus, the accurate prediction of an infectious diarrhea epidemic is crucial for public health authorities. This study was aimed at developing an optimal random forest (RF) model, considering meteorological factors used to predict an incidence of infectious diarrhea in Jiangsu Province, China.

    Get Price
  • Porcine Epidemic Diarrhea (PED) - AASV

    2013-7-26 · Porcine Epidemic Diarrhea (PED) OVERVIEW. The porcine epidemic diarrhea (PED) virus was first recognized in the United Kingdom in 1971 and had spread throughout much of Europe and Asia by 2013. The United States Department of Agriculture’s (USDA) National Veterinary Services Laboratories (NVSL)

    Get Price
  • Molecular characterization and phylogenetic analysis of ...

    2017-3-9 · Porcine epidemic diarrhea (PED) is a disease that is considered to be devastating for pig farmers with a rapid spread of infection (Wang et al., 2013; Alonso et al., 2014; Jung and Saif, 2015). The clinical symptoms of PED include severe enteritis, vomiting, and watery diarrhea, with high infectivity and lethality in piglets.

    Get Price
  • A simple and rapid identification method for newly

    2017-9-21 · Porcine Deltacoronavirus (PDCoV) is a newly emerged enteropathogenic coronavirus that causes diarrhea and mortality in neonatal piglets. PDCoV has spread to many countries around the world, leading to significant economic losses in the pork industry. Therefore, a rapid and sensitive method for detection of PDCoV in clinical samples is urgently needed.

    Get Price
  • Multiplex Reverse Transcription-PCR for Rapid

    2006-5-1 · Jung, K, Chae, C: 2005, RT-PCR-based dot blot hybridization for the detection and differentiation between porcine epidemic diarrhea virus and transmissible gastroenteritis virus in fecal samples using a non-radioactive digoxigenin cDNA probe.

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  • Understanding connectivity during an animal

    Researchers revisit the Canadian 2014 porcine epidemic diarrhea outbreak using network analysis to examine connections. By Samantha McReavy. Porcine epidemic diarrhea virus is a contagious virus that affects pigs. While pigs of any age are affected, nursing pigs are most susceptible to the infection.

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  • Porcine Epidemic Diarrhea - AASV

    Porcine epidemic diarrhea in Canada: An emerging disease case study-- Dr. Harpreet S. Kochhar, Chief Veterinary Officer, provides a synopsis of the porcine epidemic diarrhea virus outbreak and response in Canada. Pork Checkoff PEDv Update-- Weekly update by National Pork Board on PEDv issues.

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  • 1, 1 3 1, - MDPI

    2020-3-1 · 38 CoVs were conducted using MEGA7 [17]. The evolutionary distances were calculated using the Maximum Composite Likelihood method [18]. 3. Results The di erences and similarities of clinical characteristics between COVID-19 and SARS were summarized in Table1. Table 1. Comparison of SARS and COVID-19. Items SARS COVID-19

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  • A mathematical analysis of ongoing outbreak

    2020-10-26 · A newly proposed technique called q-Homotopy analysis transform method (q-HATM) is hired to find the solution for the nonlinear system portraying projected model and also presented the existence and uniqueness of the obtained results with help of fixed point theory. The behavior has been captured with respect to fractional order and time ...

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  • Laboratory Processing of Fecal Specimens

    of unknown origin. Only a few pathogens cause epidemic diarrhea, although many cause sporadic diarrhea.S. dysenteriae serotype 1 and V. cholerae are the two etiologic agents responsible for most epidemic diarrhea in the developing world, contributing substantially to the burden of morbidity and mortality.S. Typ hi, the

    Get Price
  • Killed whole-genome reduced-bacteria surface

    2021-5-4 · We report a vaccine platform to express vaccine antigens on the surface of genome-reduced bacteria to enhance vaccine immunogenicity. We demonstrate the utility of this vaccine platform by expressing the highly conserved fusion peptide (FP) of SARS-CoV-2 and porcine epidemic diarrhea virus on the surface of Escherichia coli to produce killed whole-cell bacterial vaccines.

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  • Survival of porcine epidemic diarrhea virus (PEDV) in ...

    2017-9-19 · Infection with Porcine Epidemic Diarrhea Virus (PEDV) causes vomiting, diarrhea, and dehydration in young pigs. The virus made its first appearance in the U.S. in 2013, where it caused substantial neonatal mortality and economic losses in the U.S. pork industry. Based on outbreak investigations, it is hypothesized that the virus could be transmitted through contaminated feed or …

    Get Price
  • Steps to Creating a Basic Epidemic Curve Using Microsoft ...

    2011-9-13 · Steps to Creating a Basic Epidemic Curve Using Microsoft Excel 20 07 7 Step 6a – Select the Insert ribbon, then press and hold the Columns button to bring up the Columns submenu. Under 2-D column select the middle button for the two-dimensional chart option. [Note: Is not necessary to use 3-D chart options for epidemic curves as it makes reading

    Get Price
  • Development of a Conventional RT-PCR Assay for

    2018-11-6 · Porcine deltacoronavirus (PDCoV) is a newly discovered coronavirus, which belongs to the family Coronaviridae. It causes watery diarrhea, vomiting, and dehydration in newborn piglets. A sensitive RT-PCR method is urgently required to detect PDCoV infection. In this study, we developed and evaluated a conventional RT-PCR assay and a SYBR green-based real-time RT-PCR assay that …

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  • Microarray analysis reveals the inhibition of intestinal ...

    2019-12-24 · Porcine epidemic diarrhea (PED) virus (PEDV), known as the pathogen of PED, has catastrophic impacts on the global pig industry and causes great economic losses to …

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  • Using experimental gaming simulations to elicit risk ...

    2020-3-17 · Failing to mitigate propagation of disease spread can result in dire economic consequences for agricultural networks. Pathogens like Porcine Epidemic Diarrhea virus, can quickly spread among producers. Biosecurity is designed to prevent infection transmission. When considering biosecurity investments, management must balance the cost of protection versus the consequences of …

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  • Study of a Fractional-Order Epidemic Model of

    2021-3-19 · Xueyu Wang, Xin Xu, Jun Ji * et al. Visual detection of porcine epidemic diarrhea virus using a novel reverse transcription polymerase spiral reaction method. BMC Veterinary Research. 2019. ( 中科院三区,影响因子: 1.792 ;通讯作者 )

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  • Rooting out rumors, epidemics, and crime -- with

    In this article, we discuss the existence and uniqueness of the solution of the fractional-order epidemic model of childhood diseases by using fixed point theory. The technique of natural transform coupled with the Adomian decomposition is used to find the solution of the proposed model. At the end of the article, the model is demonstrated with appropriate numerical and graphical description.

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