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tilmicosin phosphate 25 premix china using method

  • Tilmicosin Factory - China Tilmicosin Manufacturers, Suppliers

    2021-5-10 · 25% Tilmicosin Phosphate Solution. Basic Info Model No.: 25% Varieties: Infectious Disease Prevention Medicine Component: Chemical Synthetic Drugs Type: The First Class Pharmacodynamic Influential Factors: Animal Species Storage Method: Prevent ThrowIing Expired Veterinary Drugs Additional Info Packaging: 15 barrels/carton 100ml 250ml 500ml ...

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  • China Tilmicosin, Tilmicosin Manufacturers, Suppliers ...

    Tilmicosin manufacturers & suppliers. Recommended product from this supplier. GMP Veterinary Medicine Factory 20% Coated Tilmicosin Premix Granule Used for Pig, Chicken, Cow, Sheep. FOB Price: US 16.8-17.35 kg. Min. Order: 10000 kg. Varieties: Infectious Disease Prevention Medicine. Component: Chemical Synthetic Drugs.

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  • TILMICOSIN Shixin Xu, Beijing, China Dieter Arnold, Berlin ...

    2015-10-22 · C46H83N2O17P (tilmicosin phosphate) Molecular weight: 869.133 [g/mol] (tilmicosin) 967.128 [g/mol] (tilmicosin phosphate) OTHER INFORMATION ON IDENTITY AND PROPERTIES Active ingredient: Tilmicosin is a white to off-white solid comprised of a cis isomer and a diastereomeric pair of trans isomers. The ratio of cis to trans isomers is about

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  • Tilmicosin enteric granules and premix to pigs ...

    A modified rapid, sensitive HPLC method was used for determining the concentrations of tilmicosin in plasma. Pharmacokinetic parameters were calculated by using WinNonlin 5.2 software. The MIC 90 of tilmicosin against Haemophilus parasuis, Actinbacillus pleuropneumoniae, and Pasteurella multocida were all 8 μg/ml. These results indicated that these common pig respiratory bacteria …

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  • Tilmicosin | C46H80N2O13 - PubChem

    The invention solves the problem that tilmicosin phosphate is required to prepare the tilmicosin solution in the prior art and makes up for the shortage of the prior art. The invention relates to a tilmicosin solution, mainly comprising injection water, tilmicosin and sodium bisulfite; 100ml of injection water contains 7.5-20g of tilmicosin and 0.05-0.2g of sodium bisulfite.

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  • CN101584657A - Tilmicosin solution and preparation

    A live HS vaccine prepared using an avirulent P. multocida B:3,4 (fallow deer) strain has been used for control of the disease in cattle and buffaloes over 6 months of age in Myanmar since 1989; it is administered by intranasal aerosol application The vaccine has been recommended by the Food and Agriculture Organization of the United Nations as a safe and potent vaccine for use in Asian …

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  • Tilmicosin - an overview | ScienceDirect Topics

    2011-4-1 · However, tilmicosin is forbidden for usage as feed additive in China. In case of the abuse of the drug in feed and for food safety concerns, a simple and reproducible analytical method is necessary for the determination of tilmicosin in variety of feeds.

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  • Molecularly imprinted solid-phase extraction for ...

    Main Products. Tilmicosin Phosphate 20% Nosiheptide 1% Premix, Maduramycin 0.75%+Nicarbazin 8% Ceftiofur HCl, Potassium Bicarbonate Potassium Chloride, Decoquinate 6% Coccidiostates for The Poultry, Enramycin 4% 8% 10% Salinomycin 12% Premix, Florfenicol 4% Nicarbazin 8% +Monensin 8% Premix, Robenidine 6.6% 10% Robenidine HCl, Amprolium HCl 20% 30% ...

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  • Monensin Sodium-China Monensin Sodium Manufacturers ...

    2019-5-25 · A high-performance liquid chromatography (HPLC) method with detection at 290 nm was modified and validated for the determination of tilmicosin residues in broiler chicken tissues. The limits of detection (LOD) of the method were 0.01 μg/g for …

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  • Find Unimaginable Offers on Premium tilmicosin oral ...

    Tilmicosin Oral Solution Tilmicosin Tilmicosin Phosphate 25% Oral Solution With CAS 137330-13-3. US 30.00-62.00 Kilogram. 1 Kilogram (Min. Order) 4 YRS Xi'an Harmonious Natural Biotechnology Co., Ltd. 96.4%.

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  • Find Unimaginable Offers on Premium tilmicosin phosphate ...

    The tilmicosin phosphate powder will sustain them in a sparkling state of amazing health. ... Oral solution usp 25% tilmicosin phosphate powder tilmicosin for veterinary drugs. US 65.00-75.00 Kilogram. 1.0 Kilograms ... Veterinary drugs Tilmicosin Premix tilmicosin phosphate powder for pig. US 1.86-1.87 Piece. 10000 Pieces ...

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  • Premix Factory, Custom Premix OEM/ODM ... - Made-in

    You can buy factory price premix from a great list of reliable China premix manufacturers, suppliers, traders or plants verified by a third-party inspector. ... Nicarbazine Premix 25% Factory Supply High Quality. ... Tilmicosin Phosphate 20% Premix 100g/200g/500g. Tilmicosin Phosphate 20% Premix 100g/200g/500g.

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    China Nutrient Premix, China Nutrient Premix Suppliers and Manufacturers Directory - Source a Large Selection of Nutrient Premix Products at vitamin premix,soft serve premix,mineral premix for fish from China Alibaba.com ... 25% gmp supplier Tilmicosin phosphate water soluble powder poultry premix medicine Veterinary Drug Tilmicosin.

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  • Destomycin A Premix - China - Manufacturer - Premix ...

    2021-5-22 · China, Destomycin A (CAS NO.14918-35-5) Molecular Formula: C20H37N3O13 Molecular Weight: 527.60g/mol Mol File: 14918-35-5.mol Boiling point: 897.6 °C at 760 mmHg Flash Point: 496.7 °C Density: 1.67

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  • Tilmicosin enteric granules and premix to pigs ...

    2019-2-22 · The relative bioavailability of tilmicosin enteric granules to premix was 114.97 ± 7.19%, according to the AUC 0‐t values. These results demonstrated that tilmicosin enteric granules produced faster tilmicosin absorption, slower elimination, larger tissue distribution, and higher bioavailability compared to the tilmicosin premix.

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  • Tilmicosin - an overview | ScienceDirect Topics

    Mark G. Papich DVM, MS, DACVCP, in Saunders Handbook of Veterinary Drugs (Fourth Edition), 2016 Pharmacology and mechanism of action. Tildipirosin is an antibacterial of the macrolide class. It is a 16-membered ring (tilmicosin also is a 16-member ring molecule) macrolide antimicrobial with three charged nitrogen atoms (tulathromycin also has 3 charged nitrogen atoms).

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  • Molecularly imprinted solid-phase extraction for ...

    2011-4-1 · A simple, sensitive and reproducible molecularly imprinted solid-phase extraction (MISPE) coupled with high performance liquid chromatographic method was developed for monitoring tilmicosin in feeds. The polymers were prepared using tylosin as mimic template molecule, methacrylic acid as functional monomer and ethylene glycol dimethacrylate as ...

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  • Lincomycin Hydrochloride 11% Premix - China -

    2021-7-11 · /china/ml/0-s-i-1/Search.html /china/pl/0-s-i-1/Search.html /china/ppl/0-s-i-1/Search.html /china/suppliers/0-s-i-1/Search.html Search keyword should at least 2 ...

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  • Products Factory - China Products Manufacturers, Suppliers

    2021-5-10 · Tilmicosin Phosphate Solution 25% Poultry. Basic Info Model No.: 25% 30% Varieties: General Disease Prevention Medicine Component: Chemical Synthetic Drugs Type: The First Class Pharmacodynamic Influential Factors: Repeated Medication Storage Method: Moisture Proof Additional Info Packaging: 15 barrels/carton 100ml 250ml 500ml 1000ml ...

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  • Tilmicosin Factory - China Tilmicosin Manufacturers, Suppliers

    2021-5-10 · Tilmicosin Manufacturers, Factory, Suppliers From China, We are now looking forward to even greater cooperation with overseas customers based on mutual benefits. If you are interested in any of our products, please feel free to contact us for more details.

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  • China Tilmicosin Phosphate Premix 20% Manufacturers and ...

    2021-6-1 · we can easily offer you high-quality products and solutions, competitive rate and very best shopper support. Our destination is 'You come here with difficulty and we give you a smile to take away' for Tilmicosin Phosphate Premix 20%, Tylosin Tartrate Injection Veterinary Uses, Iron Dextran Injection Uses, Amoxicillin Injection For Cats, Adhering to the business philosophy of …

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  • Tilmicosin enteric granules and premix to pigs ...

    2019-2-22 · The relative bioavailability of tilmicosin enteric granules to premix was 114.97 ± 7.19%, according to the AUC 0‐t values. These results demonstrated that tilmicosin enteric granules produced faster tilmicosin absorption, slower elimination, larger tissue distribution, and higher bioavailability compared to the tilmicosin premix.

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  • Preparation, characterization, and pharmacokinetics of ...

    2020-12-1 · Tilmicosin (TMS) is a macrocyclic antibiotic specially used in veterinary clinics, but its extreme bitterness limits its use. This study aimed to obtain a taste-masked formulation of TMS by hot melt extrusion (HME) technology and to investigate the formulation’s characterization, stability, and effects in vitro/in vivo.Eudragit® E PO was selected as the carrier, and TMS dissolution in ...

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  • China Halofuginone Hydrobromide Premix Manufacturer

    Getting started; Halofuginone Hydrobromide Premix; Halofuginone Hydrobromide Premix - Factory, Suppliers, Manufacturers from China. As a way to provide you advantage and enlarge our organization, we even have inspectors in QC Crew and guarantee you our greatest assistance and product or service for Halofuginone Hydrobromide Premix, Cyromazine, Formic Acid, Bha + …

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  • Premix Manufacturers & Suppliers, China premix ...

    premix manufacturer/supplier, China premix manufacturer & factory list, find qualified Chinese premix manufacturers, suppliers, factories, exporters & wholesalers quickly on Made-in-China.com.

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  • Efficacy of enteric‐coated tilmicosin granules in pigs ...

    2019-10-7 · Experimental drug: enteric-coated tilmicosin granules, grey-white symmetrical microencapsulated granules (the average particle size is about 1 mm), insoluble in water, coated with acrylic resin, containing 20% of the active ingredient tilmicosin, produced by Ringpu Biopharmaceutical Co., Ltd. (Tianjin, China), batch number: 121,001.

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  • China Dicalcium Phosphate, Dicalcium Phosphate ...

    China Dicalcium Phosphate manufacturers - Select 2021 high quality Dicalcium Phosphate products in best price from certified Chinese Phosphate Fertilizer, Ammonium Phosphate suppliers, wholesalers and factory on Made-in-China.com

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  • Veterinary - Qingdao Fraken International Trading Co., Ltd ...

    China Veterinary catalog of Good Quality Cp Albendazole & Triclabendazole, Oxytetracycline HCl + Neomycin Sulphate + Vitamins Soluble Powder provided by China manufacturer - Qingdao Fraken International Trading Co., Ltd., page1.

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  • Pharmacovigilance of Tilmicosin in Mice - UFRGS

    2015-11-7 · mL at pH 7 and 25°C. Tilmicosin was obtained as the patent preparation Pneumotac ®1 that is a subcutaneous therapy for pneumonia and other respiratory disease conditions in cattle and sheep, formulated as 100 mL amber glass vials containing 333.828 mg tilmicosin phosphate/mL, equivalent to 300 mg tilmicosin/mL.

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  • The Epidemiologic and Pharmacodynamic Cutoff Values

    2016-3-22 · The aim of this study was to establish antimicrobial susceptibility breakpoints for tilmicosin against Haemophilus parasuis, which is an important pathogen of respiratory tract infections.The minimum inhibitory concentrations (MICs) of 103 H. parasuis isolates were determined by the agar dilution method. The wild type (WT) distribution and epidemiologic cutoff …

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  • Two-Dimensional HPLC Determination of Water-Soluble ...

    2020-8-12 · Mobile Phase: A. 25 mM phosphate buffer (dissolve ~3.4 g KH 2 PO 4 in 1 L water and adjust the pH to 3.0 with H 3 PO 4) B. CH 3 CN Gradient: See Table 3 Flow Rate: 0.8 mL/min Inj. Volume: 10 μL Temperature: 25 °C Detection: UV, absorbance at 210 and 254 nm Second Dimension Column: Acclaim 120 C18, 5 μm Analytical, 4.6 × 150 mm (P/N 059148)

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  • Development of a Quantitative Multi-Class Method for 18 ...

    2012-3-24 · The method described here is suitable for the simultaneous detection and confirmation of 18 multi-class antibiotics (11 macrolides, 4 β-lactams, 2 lincosamides, and orbifloxacin) in muscle. The developed procedure is a rapid method that has been applied to analyze commercial marketed meat in Taiwan.

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  • Identification and characterization of inorganic

    2018-3-27 · Soil pH was measured using a 1:2.5 (w/v) suspension of dry soil: water and a XL60 pH meter (Fisher Scientific, USA) (Shen et al. 2008).The amounts of total P and available P (Olsen P) were determined using the molybdate-blue method (Murphy and Riley 1962) and sodium bicarbonate extraction (Olsen et al. 1954) after acid digestion (Parkinson and Allen 1975), …

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  • Phosphate solubilizing bacteria stimulate wheat ...

    2020-4-25 · then total N was determined using the modified Kjeldahl method (Baker & Thompson, 1992). Plant P content was determined using the standard vanadomolybdate method (Hanson, 1950). Available P of soil was extracted with resin and measured following (Hedley & Stewart, 1982). Biological N 2 fixation assay

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  • Fabrication of calcium phosphate 3D scaffolds for bone ...

    2020-3-4 · The calcium phosphate particles can be used as building blocks for fabrication of 3D scaffolds intended for bone tissue engineering. This work presents for the first time a …

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  • The International Pharmacopoeia - WHO

    2021-4-29 · The International Pharmacopoeia (Ph. Int.) constitutes a collection of recommended procedures for analysis and specifications for the determination of pharmaceutical substances and dosage forms that is intended to serve as source material for reference or adaptation by any WHO Member State wishing to establish pharmaceutical requirements. The pharmacopoeia, or any …

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  • Probing the active fraction of soil microbiomes using ...

    2020-2-18 · GC conditions: The analytes were separated using an HP-5MS column (30 m × 0.25 mm I.D. × 0.25 μm film thickness; Agilent Technologies, USA); the carrier gas was high-purity helium (99.999%, Airgas Inc.); the flow rate was 1 mL/min; the injector temperature was 280 °C; The injection mode was split, and the split ratio was 10:1.

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  • China Tilmicosin Dose In Poultry Factory and Manufacturers ...

    2021-4-20 · Tilmicosin Dose In Poultry Manufacturers, Factory, Suppliers From China, Our aim is to help customers realize their goals. We are making great efforts to achieve this win-win situation and sincerely welcome you to join us!

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  • China Noromectin Premix Manufacturers and Factory ...

    2021-2-21 · The corporation upholds the philosophy of 'Be No.1 in high quality, be rooted on credit rating and trustworthiness for growth', will continue to serve outdated and new consumers from home and overseas whole-heatedly for Noromectin Premix, Specinomycin And Lincomycin Injection, Praziquantel Oral Solution 2.5%, Enrofloxacin Injection Uses, We can customize the products …

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  • Pharmacovigilance of Tilmicosin in Mice - UFRGS

    2015-11-7 · mL at pH 7 and 25°C. Tilmicosin was obtained as the patent preparation Pneumotac ®1 that is a subcutaneous therapy for pneumonia and other respiratory disease conditions in cattle and sheep, formulated as 100 mL amber glass vials containing 333.828 mg tilmicosin phosphate/mL, equivalent to 300 mg tilmicosin/mL.

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  • Compendium of Medicating Ingredient Brochures until March ...

    2018-3-31 · approved brands of premix containing that medicating ingredient and their respective levels of the medicating ingredient. Please note that these are the only brands approved for use in Canada. Using other unapproved sources of the drug in feed is a contravention of the Feeds Act and Feeds Regulations and the Food and Drugs Act and Regulations.

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  • Comparison of two methods for RNA extraction from the ...

    2016-6-2 · digestion method, the mean RNA concentration was 898.42 ± 38.64 ng/mL, the mean OD ratio was 1.98 ± 0.28, and the positive rate was 100% (Table 1). The mean RNA concentration obtained using the enzyme digestion method was significantly higher than that using the liquid nitrogen-grinding method (P < 0.05). OD = optical density. Table 1.

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  • A one step method for isolation of genomic DNA using multi ...

    The sAMNPs were prepared according to a previously reported method, with some modifications. 20 Briefly, 0.5 g of SMNPs was dispersed for 30 min in a mixture of 475 mL ethanol and 25 mL deionized water, with the aid of ultrasonication. Then, the mixture was poured into a two-neck round-bottom flask, and 10 mL APTES was added dropwise, with ...

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  • Fast DNA Extraction with Polyacrylamide Microspheres for ...

    2020-6-3 · DNA is extracted by using a similar procedure consisted of lysis, binding, washing, and elution. This procedure usually spends at least half an hour and often needs at least four times of solution and tube transfers. However, in our method, except plant tissue, only one solution (0.4 M NaOH), one tube, and an easy two-step operation are needed.

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  • Probiotic Lactobacillus casei Shirota prevents acute liver ...

    2021-1-25 · P-values were adjusted for the false discovery rate using the Benjamini–Hochberg method where necessary (*P adj < 0.05; **P adj < 0.01; ***P adj < 0.001). Conflict of interest The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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  • Best Ways to Use TSP Cleaner | DoItYourself.com

    2010-9-1 · TSP cleaner is strong. TSP, also known as trisodium phosphate, is a pure phosphate, and like all phosphates it must be handled carefully because it can damage the environment.TSP cleaner is certainly tough enough to take on big jobs, but it should always be used responsibly, while wearing rubber gloves and a mask, and in well-ventilated areas.

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  • Targeted DNA demethylation in vivo using dCas9–peptide ...

    2016-8-29 · ESCs were transfected using systems 2–4 with Gfap_2 gRNA, harvested, and lysed in lysis buffer (25 mM Tris-HCl pH 7.4, 150 mM NaCl, 2 mM EDTA, 1% NP-40, and 5% glycerol). The cell lysate was ...

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  • Determination of Water- and Fat-Soluble Vitamins by HPLC

    2017-10-30 · 2 HPLC methods for water- and fat-soluble vitamin analysis Traditional HPLC method Reversed-phase HPLC is a well-suited technique for vitamin analysis.1 In typical regulated HPLC methods2,3 and commonly reported HPLC methods,4,5 water-soluble vitamins are determined using an aqueous mobile phase

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  • Identification and characterization of inorganic

    2018-3-27 · Soil pH was measured using a 1:2.5 (w/v) suspension of dry soil: water and a XL60 pH meter (Fisher Scientific, USA) (Shen et al. 2008).The amounts of total P and available P (Olsen P) were determined using the molybdate-blue method (Murphy and Riley 1962) and sodium bicarbonate extraction (Olsen et al. 1954) after acid digestion (Parkinson and Allen 1975), …

    Get Price
  • Is oseltamivir suitable for fighting against COVID-19: In ...

    2020-9-2 · Oseltamivir is a first-line antiviral drug, especially in primary hospitals. During the ongoing outbreak of coronavirus disease 2019 (COVID-19), most patients with COVID-19 who are symptomatic have used oseltamivir. Considering its popular and important role as an antiviral drug, it is necessary to ...

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  • Hyaluronan synthase 2 expressed by cancer-associated ...

    2017-8-25 · ized to the level in the control samples using the com-parative 2-ΔΔCT method. The primer sequences (forward and reverse) are listed in Additional file 1: Table S1. Western blotting The harvested cells were lysed on ice for 30 min in RIPA Lysis Buffer (Beyotime, Shanghai, China) containing 100 mM PMSF (Beyotime) and centrifuged at 12000×g ...

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  • Phosphate solubilizing bacteria stimulate wheat ...

    2020-4-25 · then total N was determined using the modified Kjeldahl method (Baker & Thompson, 1992). Plant P content was determined using the standard vanadomolybdate method (Hanson, 1950). Available P of soil was extracted with resin and measured following (Hedley & Stewart, 1982). Biological N 2 fixation assay

    Get Price
  • Adipose-derived stem cells therapy effectively attenuates ...

    2021-6-19 · China). Read mapping and differentially expressed gene analysis As described previously [20], the raw data were cleaned with SOAPnuke and trimmomatic software [21], and then the clean reads were mapped to the reference gen-ome (Mus_musculus, GCF_000001635.25_GRCm38.p5) using HISAT [22] or Bowtie 2 [23] software. RESM soft-

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  • ZFIN

    Use glass finger bowls filled two-thirds full with system water. 2. To each bowl add 8-9 grains of of boiled wheat and 8 ml from a paramecia seed culture. 3. Stack the finger bowls 6 or 8 high, cover, and store at 28.5°C on well-lit shelves. 4.After 10-14 days, the paramecia are ready to …

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  • Dedicator of Cytokinesis 5 Regulates Keratinocyte

    2021-5-1 · Cutaneous wound healing is a fundamental biologic and coordinated process, and failure to maintain this process contributes to the dysfunction of tissue homeostasis, increasing the global burden of diabetic foot ulcerations. However, the factors that mediate this process are not fully understood. Here, we identify the pivotal role of dedicator of cytokinesis 5 (Dock5) in …

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  • Development of a qualitative real-time PCR method to ...

    2016-6-24 · In China, to adapt the analytical approach with the growing GMO environment and to cover a wider range of GM targets, the development of a new GMO multiscreening method was undertaken. In this paper, we describe a real-time PCR screening method for GMO identification, which contains an assay for the detection of 19 important genetic elements.

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  • Mechanisms of microRNA‑142 in mitochondrial autophagy and ...

    dehyde‑3‑phosphate dehydrogenase (GAP dH) for PINK1. The relative transcription levels of target genes were calculated using the 2‑ΔΔct relative quantitative method (19). ΔΔ ct=Δ t epilepsy group‑ Δct control group, ct=ct (target gene)‑ct (internal reference). Western blot analysis.

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  • Supporting Information - American Chemical Society

    2019-12-12 · Premix Ex Taq. TM. II kit were purchased from Takara Biotechnology Co. Ltd. (Dalian, China). The buffers for cell experiments were prepared using distilled water purified by a Milli-Q water purification system (Millipore Corp., Bedford, MA) with an electrical resistance of 18.2 MΩ•cm. The buffers used in cellular extracts were prepared as ...

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  • A one step method for isolation of genomic DNA using multi ...

    The sAMNPs were prepared according to a previously reported method, with some modifications. 20 Briefly, 0.5 g of SMNPs was dispersed for 30 min in a mixture of 475 mL ethanol and 25 mL deionized water, with the aid of ultrasonication. Then, the mixture was poured into a two-neck round-bottom flask, and 10 mL APTES was added dropwise, with ...

    Get Price
  • Internal Reference Gene Selection under Different Hormone ...

    2020-9-21 · using SYBRfi Premix Ex TaqŠ (TaKaRa, Guangzhou, China). The real-time PCR volume of 10 L contained 5 L 2xSYBR Premix Ex Taq II, 0.5 L primer F (5 molL1), 0.5 L primer R (5 molL1), 2 L cDNA, and 2 L ddH2O. The thermocycling conditions were as follows: 95 C for 30 s, 40 cycles

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  • Development of a qualitative real-time PCR method to ...

    2016-6-24 · In China, to adapt the analytical approach with the growing GMO environment and to cover a wider range of GM targets, the development of a new GMO multiscreening method was undertaken. In this paper, we describe a real-time PCR screening method for GMO identification, which contains an assay for the detection of 19 important genetic elements.

    Get Price
  • A modified SDS-based DNA extraction method from raw ...

    The optimized method was verified by extracting approximately 2020–2444 ng DNA/mg soybean with A 260/280 ratios of 1.862–1.954 from five biotech and non-biotech soybean varieties. Only 0.5 mg of soya was required to obtain enough DNA for PCR amplification using the optimized SDS-based method.

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  • Determination of Water- and Fat-Soluble Vitamins by HPLC

    2017-10-30 · 2 HPLC methods for water- and fat-soluble vitamin analysis Traditional HPLC method Reversed-phase HPLC is a well-suited technique for vitamin analysis.1 In typical regulated HPLC methods2,3 and commonly reported HPLC methods,4,5 water-soluble vitamins are determined using an aqueous mobile phase

    Get Price
  • Agrobacterium rhizogenes -induced soybean hairy roots ...

    2021-6-23 · tured and passaged using the tissue culture method as described in a previous study [19]. In short, the specimens were cut from the mucosa, minced, and washed twice in phosphate-buffered saline (PBS) containing antibiotics (100 U/mL penicillin, 100mg/mL streptomycin, and 0.25mg/mL amphotericin). Then, fBMFs were placed in a 60mm culture

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  • Adipose-Derived Stem Cell-Derived Extracellular Vesicles ...

    2017-6-26 · First-strand cDNA was synthesized using TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix (TransGen Biotech, China) according to the manufacturer’s instructions. Reactions were carried out using a SYBR Premix Ex Taq II (Tli RnaseH Plus) Kit (TaKaRa, Japan) in a Lightcycler 480 Real-Time PCR Detection System (Roche, Germany).

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  • Integrated analysis of transcriptome and metabolites ...

    2021-1-5 · Structural variations (SVs), a major resource of genomic variation, can have profound consequences on phenotypic variation, yet the impacts of SVs remain largely unexplored in crops. Here, we generate a high-quality de novo genome assembly for a flat-fruit peach cultivar and produce a comprehensive SV map for peach, as a high proportion of genomic sequence is …

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  • A modified SDS-based DNA extraction method from raw ...

    The optimized method was verified by extracting approximately 2020–2444 ng DNA/mg soybean with A 260/280 ratios of 1.862–1.954 from five biotech and non-biotech soybean varieties. Only 0.5 mg of soya was required to obtain enough DNA for PCR amplification using the optimized SDS-based method.

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  • A Modified SDS-Based DNA Extraction Method for High ...

    2016-6-23 · A metagenome from the GB sediment sample was constructed using DNA extracted by the M-SDS method using Hiseq 2000 platform for sequencing (conducted at the BGI-Shenzhen, China). The generated GB metagenome contained ∼30 Gbp raw data, from which metabolic potentials of the microbial community will be investigated in the future.

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  • Frontiers | Tandem UGT71B5s Catalyze Lignan

    2021-3-31 · Gene expression levels were detected using real-time quantitative PCR [qRT-PCR; TB Green® Premix Ex Taq™ (Tli RNaseH Plus), RR420A, TAKARA, China; QuantStudio™ 3, Applied Biosystems, United States]. Gene specific primers are listed in Supplementary Table S3. Each group of samples had six biological replicates, and each biological replicate ...

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  • Identification of drought tolerant mechanisms in a drought

    2020-7-9 · Frequently occurring drought stress negatively affects the production of maize worldwide. Numerous efforts have been made to develop drought-tolerant maize lines and to explore drought tolerant mechanisms in maize. However, there is a lack of comparative studies on transcriptomic changes between drought-tolerant and control maize lines. In the present study, we have developed a drought …

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  • Identification of mRNAs related to endometrium function ...

    2018-10-15 · 30 s, according to the instructions of SYBR Premix Ex Taq (TaKaRa). The mRNAs and lncRNA CD36–005 were normalized to glyceraldehyde-3-phosphate de-hydrogenase (GAPDH), and the relative expression levels were analyzed by calculating the fold changes using the 2 value method. We purchased the pri-mer sequences for qRT-PCR from RiboBio Company ...

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  • A novel quantification method for the total demethylation ...

    2015-11-26 · The demethylation potential of environmental pollutants is possibly an innate part of their comprehensive health risk. This paper develops a novel method called TDQ to quantify the demethylation epigenetic toxicity, termed the 5-AZA-CdR demethylation toxic equivalency, of aquatic samples from the heavily polluted Bohai Bay using Hep G2 cell lines transiently …

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  • IOP Conference Series: Materials Science and

    2021-1-4 · The validation results have a good agreement with the certificate value with bias (%) ranging from 0.6 to 6.9. Determination of macro and micromineral nutrients using NAA method resulted K, Na, Fe, Se, and Zn with an average value were 0.66%; 0.25%; 82.7 µg/g; 0.14 µg/g; and 34.7 µg/g respectively. These values were still within the ...

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  • Regeneration of pulpo-dentinal–like complex by a group

    Dental pulp is critical to maintain the vitality of a tooth. Regeneration of pulpo-dentinal complex is of great interest to treat pulpitis and pulp necrosis. In this study, through three-dimensional spheroid culture, a group of unique multipotent stem cells were identified from mouse dental papilla called multipotent dental pulp regenerative stem cells (MDPSCs).

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  • Inhibiting repressive epigenetic modification promotes ...

    2019-10-23 · Real-time quantitative RT-PCR was performed using an SYBR Premix Ex Taq (Takara, Kusatsu, Japan) and signals were detected with Applied Biosystems 7500 Real-Time PCR System (Thermo Fisher Scientific). Analysis of relative gene expression was measured using the 2-ΔΔCt method. Primers are shown in Supplemental Table S4.

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  • Genome structure variation analyses of peach reveal ...

    2021-1-5 · Structural variations (SVs), a major resource of genomic variation, can have profound consequences on phenotypic variation, yet the impacts of SVs remain largely unexplored in crops. Here, we generate a high-quality de novo genome assembly for a flat-fruit peach cultivar and produce a comprehensive SV map for peach, as a high proportion of genomic sequence is …

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  • IdentificationandCharacterizationofanOocyteFactor ...

    2020-12-3 · the gels and washed with a solution of 25 mM NH 4 HCO 3 and 50% acetonitrile, dehydrated with 100% acetonitrile sequen-tially, and dried by centrifugal lyophilization. The gels were digested with 15–20 l of 0.01 g l trypsin (Promega) in 25 mM ammoniumbicarbonatefor15hat37 °C.Thesupernatants werecollected ...

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  • Molecular characterization, chromosome mapping, and ...

    2015-10-19 · using SYBR Green I method (Livak and Schmittgen, 2001). The reaction mixture (25 μL) contained 2 μL RT-PCR product, 12.5 μL 2X SYBR Premix Ex Taq II, 0.5 μL each CDC16 …

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  • Sphingosine-1-Phosphate Is a Possible Fibrogenic Factor in ...

    2020-5-15 · Sphingosine-1-Phosphate Is a Possible Fibrogenic Factor in Gluteal Muscle Fibrosis C. G. ZHAO1, J. QIN2, X. J. HE2, Y. C. GUAN1, Y. JIA1, W. LEI3 1Orthopedic Center of Chinese PLA, Urumqi General Hospital of Lanzhou Military Region, Urumqi, Xinjiang, China, 2Department of Orthopedic Surgery, Second Affiliated Hospital

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  • The High-Affinity Phosphate Transporter GmPT5 Regulates ...

    2019-11-27 · Dendrobium moniliforme (Linnaeus) Swartz is a well-known plant used in traditional Chinese medicine due to bioactive constituents. Polysaccharides are the main medicinal ingredients, yet no studies have been published on polysaccharide biosynthesis in D. moniliforme. To comprehensively investigate the polysaccharide at the transcription level, we performed de …

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  • Polysaccharide biosynthetic pathway profiling and putative ...

    2017-2-20 · Using the SYBR Premix Ex Taq II Kit (Takara, Dalian, China), 20 μL qPCR mixture contained 10 μL SYBR Premix Ex Taq II, 0.8 μL forward primer, 0.8 μL reverse primer, 0.4 lb Rod Reference Dye, 2 μL cDNA and 6 μL DEPC-treated water. The qPCR samples were run on an ABI StepOne Real-Time PCR system (Applied Biosystems, San Mateo, CA, USA).

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  • High-level extracellular protein production in Bacillus ...

    2021-2-2 · Introduction. Hepatitis E virus (HEV) is one of many important agents that cause acute viral hepatitis in humans. This viral infection is linked to 20 million clinical cases annually, with 56,000 HEV deaths globally (Blasco-Perrin et al., 2016).HEV is a member of the Hepeviridae family and contains a single-stranded positive sense RNA genome, which is ∼7.2 kbp in size.

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  • Single-base mapping of m6A by an antibody-independent

    2021-5-20 · 9109, TaKaRa, China) and dissolved in RNase free water (Cat. No. 1768385, Invitrogen). The concentration and quality of RNA were determined by using NanoDrop 2000 (ThermoFisher). The RNA with 260/280 ratio of around 2 was chosen for further use. Then 100 ng RNA was reverse-transcribed to cDNA using PrimeScriptTM RT Master Mix

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  • Detection of Hepatitis E Virus in the Pig Livers and ...

    2015-2-21 · Genomic DNA was prepared by Bust n’ Grab method . Total RNA of each sample was prepared using an RNAprep pure kit (Tiangen Biotech, Co., Ltd, Beijing, China) following the manufacturer’s recommendations. RNA samples were stored at −80°C until used.

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