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rpi kanamycin monosulfatekanamycin using method

  • A method for transformation of Paracoccus

    1981-10-1 · Paracoccus denitrificans. FEMS Microbiology Letters 12 (1981) 159-161 Published by Elsevier/North-Holland Biomedical Press A method for transformation of Paracoccus denitrificans David W. Spence * and Gordon C. Barr Department of Biochemistry, University of Dundee, Dundee DDI 4HN, Scotland Received 2 July 1981 Accepted 6 July 1981 1.

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  • Construction and functional characterization of truncated ...

    2017-9-13 · grown in 3 mL of LB containing kanamycin at 37 °C for 12 h, respectively. The culture was then diluted to OD600=0.1andgrownat37°Cand0.5mMIPTGwasadded when OD600 reached 0.8, then the culture was continuously grown at 30 °C for 6 h. Cells were harvested by centrifugation at4°Cat5000 ×gfor20min.Thecellswerethenre-suspended

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  • Development of late blight resistant potatoes by

    2016-8-29 · Molecular weight analysis using PAGE and GPC One-dimensional polyacrylamide gel electrophoresis (PAGE) was chosen for molecular weight analysis of heparosan using a standard method of the Bio-Rad Mini-Protean II system (7 cm × 10 cm mini-gels). Two cross-linker acrylamide gels, 22 % and with 0.5 % with 1 mm thickness, were used for

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  • Recombinant Escherichiacoli K5 strainwiththe deletion of ...

    2011-9-28 · Here we present a method for a rapid screening of HP HS–protein interactions. A small library of polysaccharides structurally related to HP/HS was synthesized on a sensor chip using bacterially expressed C 5-epimerase and sul-fotransferases, and the ability of each polysaccharide to bind ATIII was analyzed using surface plasmon resonance (SPR).

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  • BBRC - Rensselaer Polytechnic Institute

    2018-10-1 · We generally recommend using vector pFGC5941 (plasmid information can be accessed via Table 1), which has the following key features: a kanamycin resistance (kan R) gene for bacterial selection, a basta resistance (bar) gene for plant selection, a CaMV 35S promoter to drive the expression of the inverted repeat target sequence, and a 1,352-bp ChsA intron (from the petunia Chalcone …

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  • ChromDB::Chromatin Database

    2021-7-12 · plasmids with kanamycin resistance and introduced in BL21(DE3) bacteria using the heat‐ shock method. Uniform 15N/13C labeling was obtained by growing cells in minimal M9 medium containing 15NH4Cl/13C‐u‐labeled glucose as the sole nitrogen and carbon sources.

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  • Article Comparative Assessment of NMR Probes for the ...

    2020-4-14 · By using markers obtained from RLP/KSeq in conjunction with additional markers, we fine-mapped the SCR74 receptor to a 43-kbp G-LecRK locus. Our findings show that RLP/KSeq enables rapid mapping of PRRs and is especially beneficial for crop plants with large and complex genomes. This work will enable the elucidation and characterization of the nonNLR plant immune receptors and ultimately …

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  • RLP/K enrichment sequencing; a novel method to

    2011-11-23 · DNA was extracted from plantlets using a high-throughput method (Huang et al. 2005). The PCR primers listed in Table 1 were used in standard PCR reactions with the indicated annealing temperatures. PCR products were separated in a 1% agarose gel and stained with ethidium bromide (Fig. 1).

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  • Extreme resistance to late blight disease by transferring ...

    using a phenol-chloroform extraction method (Gawel and Jarret, 1991). To confirm the presence of the T-DNA in putative ‘Tigoni’ and ‘Shangi’ events, primers overlapping the region between RB - Rpi-blb2 and RB - Rpi …

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  • Addgene: Plasmid Cloning by PCR (with Protocols)

    1974-7-10 · Escherichia coli gene transfer to unrelated bacteria by a histidine operon — RP1 drug resistance plasmid complex gene transfer to unrelated bacteria by a histidine operon — RP1 drug resistance plasmid complex

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  • Escherichia coli gene transfer to unrelated bacteria by

    2021-6-7 · theos A. G. Koffas at [email protected] VC 2015 American Institute of Chemical Engineers 1. conditions including media, temperature, promoter strengths, substrate timing, and induction points. Using optimum condi-tions, we confirm the previously published high titer conver-

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  • Rpi - blb2 gene from Solanum bulbocastanum confers

    2019-5-25 · using a NanoDrop ND-1000 spectrophotometer. DNA sequencing was performed at the University of Michigan DNA Sequencing Core. E. coli DH5α was used for plasmid preparation and maintenance while E. coli BL21(DE3) was used for protein overexpression. Chemically competent E. coli cells were prepared using the method of Inoue.1 Optical density (OD ...

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  • Optimization of Naringenin and p ... - homepages.rpi.edu

    2012-8-21 · One of the most ambitious goals in metabolic engineering is the design of biological systems based on in silico predictions using mathematical models. The advent of high-throughput technologies and the completion of genome sequencing for many organisms have led to an explosion of systems-wide biological data [1, 2].Genome-scale stoichiometric models of the increasing number of microorganisms ...

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  • Biochemical and Structural Characterization of MycCI, a ...

    The composition can be a feedstock, for example. Still further provided is a method of using the feedstock to manufacture a composition, such as a polymer, such as a polyester, a detergent, a surfactant, a solvent, a paint, a varnish, a lubricant, a cosmetic, and the like. ... pDEST_KASIIIA, and pDEST_KASIIIB or 50 μg/ml kanamycin (RPI Corps ...

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  • Flux variability scanning based on enforced objective

    a co-culture method was employed for the production of curcuminoids from glucose in a lab scale bioreactor. This system provides a new method transforming inexpensive substrate into value-added products. 1 Introduction The rhizome of turmeric (Curcuma longa) is an important medicinal and flavoring agent in Asian countries.

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  • MATERIALS AND METHODS FOR CHARACTERIZING

    2011-11-23 · Phytophthora infestans is the causal agent of late blight in potato. The Mexican species Solanum demissum is well known as a good resistance source. Among the 11 R gene differentials, which were introgressed from S. demissum, especially R8 and R9 differentials showed broad spectrum resistance both under laboratory and under field conditions. In order to gather more information about …

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  • Improvement of late blight resistance on potato by ...

    3. Functional detection of Rpi-sto1 and Rpi-vnt1.1 genes in plants produced from different transformations was carried out in experiment 3. Agro-infiltration carried out with the Avr effectors was co-related with the PCR detection of relevant R genes. By using different Pythophthora isolates in detached leaf

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  • Addgene: Plasmid Cloning by PCR (with Protocols)

    2019-5-25 · Chemically competent E. coli cells were prepared using the method of Inoue4 or using a Z-competent E. coli transformation buffer kit (Zymo Research). Optical density (OD 600) was measured using an Eppendorf BioPhotometer for cell growth and a Molecular

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  • Chemoenzymatic total synthesis and structural ...

    To assess the relative expression values of each of the 3R genes, qPCR reactions were set up using Luna® Universal Probe qPCR Master Mix with gene specific primer sets (Table 1) and qRT-Rpi-vnt1.1-F and qRT-Rpi-vnt1.1-R for Rpi-vnt1.1 gene (Roman et al., 2017), qRT-RB-F and qRT-RB-R for RB gene (Kramer et al., 2009), qRT-Rpi-blb2- and qRT-Rpi ...

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  • African Journal of Biotechnology - extreme resistance

    2019-5-25 · S5 S. fradiae genomic DNA using primers P4_forward/P4_reverse (start site 1) and P5_forward/P5_reverse (start site 2). Following isolation and purification of the DNA amplicons, restriction digestion was carried out with NdeI and HindIII.The cut tylHI fragments were then ligated into pET28b that had previously been digested with the same restriction enzymes to

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  • Biochemical and Structural Characterization of MycCI, a ...

    2020-9-23 · Phylogenetic analysis using neighbor-joining (NJ) method showed that these proteins were divided into two main branches of AG and AGL11 lineages corresponding to the MADS-box class C and class D genes, respectively (Fig. 2). The first WGDs was found to have occurred during evolutionary history of marigold.

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  • Identification, characterization and functional analysis ...

    2017-4-13 · (RPI corp, Mount Prospect, IL #T36000) were prepared using 5 g L 1 Phyto agar (RPI Corp #A20300) as the gelling agent. Phyto agar (CAS Number 9002-18-0) was used as the gelling agent for the paromomycin containing media, instead of Phytagel (CAS Number 71010-52-1), following a previous report that paromomycin precipitates from media containing

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  • Use of Agrobacterium rhizogenes Strain 18r12v and ...

    using bovine serum albumin as a standard. DNA isolation, manipulation and transformation Genomic DNA of Bifidobacterium was isolated according to the method of Kim et al. (2004b). Routine DNA manipulations were performed according to standard pro-cedures as …

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  • Synthetic non-oxidative glycolysis enables complete

    2012-3-1 · Medical AbstractsKeyword: tuberculosis Publication date from 2012/03/01 to 2012/03/311. PLoS One. 2012;7(3):e33886. Epub 2012 Mar 29.Identification of Novel Mt-Guab2 Inhibitor Series Active against M.tuberculosis.Usha V, Hobrath JV, Gurcha SS

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  • Direct Detection of Bacterial Protein Secretion Using ...

    1 μl of each colony filtrate was spotted onto a 384-well Gold MALDI target (Bruker Daltronics, Billerica, MA) using the dried overlay method and allowed to evaporate under vacuum. 1 μl of matrix, saturated sinapinic acid (Sigma-Aldrich) in 50% acetonitrile, 0.1% TFA …

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  • Cloning and exploitation of a functional R-gene from ...

    2018-1-2 · The method according to claim 8, wherein said plant is also provided with a nucleic acid encoding a resistance protein selected from the group of Rpi-blb1, Rpi-blb2, Rpi-blb3, Rpi-vnt1, Rpi-chc1, Rpi-edn1, Rpi-edn3, Rpi-mcq1 and combinations thereof.

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  • Use of Agrobacterium rhizogenes Strain 18r12v and ...

    2017-4-13 · (RPI corp, Mount Prospect, IL #T36000) were prepared using 5 g L 1 Phyto agar (RPI Corp #A20300) as the gelling agent. Phyto agar (CAS Number 9002-18-0) was used as the gelling agent for the paromomycin containing media, instead of Phytagel (CAS Number 71010-52-1), following a previous report that paromomycin precipitates from media containing

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  • High-Resolution Sequence-Function Mapping of Full

    2015-3-19 · Comprehensive sequence-function mapping involves detailing the fitness contribution of every possible single mutation to a gene by comparing the abundance of each library variant before and after selection for the phenotype of interest. Deep sequencing of library DNA allows frequency reconstruction for tens of thousands of variants in a single experiment, yet short read lengths of …

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  • Systematic phenome analysis of Escherichia coli

    2009-9-15 · Using a new method employing a P1 phage derivative, we created systematic double-deletion mutants in the central carbon metabolism of E. coli. The mutants were created by combining 31 single-gene deletions (first deletion) with deletions in seven key reactions (second deletion).

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  • Improving production of Streptomyces griseus trypsin

    2020-4-13 · Background Trypsin has many applications in food and pharmaceutical manufacturing. Although commercial trypsin is usually extracted from porcine pancreas, this source carries the risks of infectivity and immunogenicity. Microbial Streptomyces griseus trypsin (SGT) is a prime alternative because it possesses efficient hydrolysis activity without such risks. However, the remarkable …

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  • Lidstrom:Autoinduction Media - OpenWetWare

    2021-2-27 · About Autoinduction media. Glucose and the inducing sugar are present in the media while the cells grow. In the beginning, the cells prefer glucose. As the glucose runs out, the cells start taking up the induction sugar (usually lactose), causing induction of genes related to that sugar's metabolism (e.g. lac promoter, pTrc promoter, T7 promoter).

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  • Cisgenics for crop improvement - SlideShare

    2021-5-6 · The evolutionary distances were computed using the p-distance method (Nei and Kumar, 2000) and were in the units of the number of amino acid differences per site. The robustness of the tree branches was assessed by performing bootstrap analysis of the Neighbour-Joining data based on 1000 resamplings (Felsenstein, 1985 ).

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  • New insights into hydroxyectoine synthesis and its ...

    2016-7-14 · Labeling of proteins with fluorinated amino acids and using fluorine-19 NMR greatly simplifies the NMR spectrum. Changes in the spectrum on interaction …

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  • Improvement of late blight resistance on potato by ...

    3. Functional detection of Rpi-sto1 and Rpi-vnt1.1 genes in plants produced from different transformations was carried out in experiment 3. Agro-infiltration carried out with the Avr effectors was co-related with the PCR detection of relevant R genes. By using different Pythophthora isolates in detached leaf

    Get Price
  • Generation and Use of Recombinant Galectins - Wu -

    2021-3-3 · Dissolve 2.5 g kanamycin sulfate from Streptomyces kanamyceticus (MilliporeSigma, K1377) in 50 ml sterilized water. Pass through a 0.22-µm filter and aliquot into 1.5-ml vials and store at −20°C for up to 6 months. LB broth. Dissolve 20 mg of LB broth powder (RPI Research Products International, L24060) in 900 ml H 2 O.

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  • Chemoenzymatic total synthesis and structural ...

    2019-5-25 · Chemically competent E. coli cells were prepared using the method of Inoue4 or using a Z-competent E. coli transformation buffer kit (Zymo Research). Optical density (OD 600) was measured using an Eppendorf BioPhotometer for cell growth and a Molecular

    Get Price
  • Genome-wide identification and functional analysis of

    2021-2-2 · Relative expression was calculated using the 2 – CT method. The activities of the main disease-resistance enzymes, including ROS, SOD, POD, and CAT, were determined using the ROS Assay Kit E004–1-1, SOD Assay Kit A001–3-2, POD Assay Kit A084–3 and CAT Assay Kit A007–1-1 (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) with ...

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  • RLP/K enrichment sequencing; a novel method to identify ...

    Methods RLP/K enrichment sequencing; a novel method to identify receptor-like protein (RLP) and receptor-like kinase (RLK) genes Xiao Lin1, Miles Armstrong2, Katie Baker2, Doret Wouters1, Richard G. F. Visser1, Pieter J. Wolters1, Ingo Hein2,3* and Vivianne G. A. A. Vleeshouwers1* 1Plant Breeding, Wageningen University and Research, Droevendaalsesteeg 1, 6708 PB, Wageningen, the …

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  • Engineeringmetabolismandproductformationin

    2021-6-7 · MetabolicEngineering5(2003)32–41 EngineeringmetabolismandproductformationinCorynebacterium glutamicum bycoordinatedgeneoverexpression MattheosA.G.Koffas ...

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  • Cisgenics for crop improvement - SlideShare

    The composition can be a feedstock, for example. Still further provided is a method of using the feedstock to manufacture a composition, such as a polymer, such as a polyester, a detergent, a surfactant, a solvent, a paint, a varnish, a lubricant, a cosmetic, and the like. ... pDEST_KASIIIA, and pDEST_KASIIIB or 50 μg/ml kanamycin (RPI Corps ...

    Get Price
  • MATERIALS AND METHODS FOR CHARACTERIZING

    2011-11-23 · Phytophthora infestans is the causal agent of late blight in potato. The Mexican species Solanum demissum is well known as a good resistance source. Among the 11 R gene differentials, which were introgressed from S. demissum, especially R8 and R9 differentials showed broad spectrum resistance both under laboratory and under field conditions.

    Get Price
  • Broad spectrum late blight resistance in potato ...

    2020-3-18 · The immune system encounters many bacteria that are infected with dormant viruses (phages) that have the potential to “reawaken” and lyse their host. To observe the nature of these interactions, we set up a platform to image the phage λ with a fluorescent reporter for lysis inside E. coli engulfed by macrophages. We visualized macrophages triggering internalized E. coli to induce λ and …

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  • Kanamycin Monosulfate, USP Grade | GoldBio

    Product Description. Kanamycin is an aminoglycoside antibiotic isolated from Streptomyces kanamyceticus. It is effective against gram-positive and gram-negative bacteria as well as Mycoplasma species. Kanamycin is commonly used to select for bacteria that have been transformed with a kanamycin-resistant gene and plant tissues that have the NPT ...

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  • Kanamycin Monosulfate 50 mg/mL Solution | GoldBio

    Consequently, protein synthesis is inhibited. GoldBio’s antibiotic solutions are provided ready to use. Product Specifications: Kanamycin Monosulfate in H 2 O. (50 mg/mL Premade Solution, 0.2 µm Sterile Filtered) MOLECULAR BIOLOGY GRADE. Formula: C 18 H 36 N 4 O 11 · H 2 SO 4. MW: 582.58 g/mol.

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  • (PDF) Nucleotide sequence of the kanamycin

    IncPa plasmids including the Bir- (Barth and Grinter, 1977; Grinsted et al., mingham plasmids isolated from a variety of 1978) and by the observation that insertion gram-negative bacteria species always confer of DNA fragments into the unique Hind111 kanamycin and tetracycline resistances to site of RK2 in vitro results in a kanamycin- their hosts.

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  • Physicochemical Factors That Favor Conjugation of an ...

    2019-10-16 · Hinton broth (Fisher Scientific, Waltham, MA, USA) using the method similar to European Committee for Antimicrobial Susceptibility Testing (EUCAST) of the European Society of Clinical Microbiology, and Infectious Diseases (ESCMID) (2003) by diluting antibiotics 1:10 from 100 µg to 0.01µg/ml (SupplementaryTable1). The goal of the MIC assays ...

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  • Genome-wide identification and functional analysis of

    2021-2-2 · Relative expression was calculated using the 2 – CT method. The activities of the main disease-resistance enzymes, including ROS, SOD, POD, and CAT, were determined using the ROS Assay Kit E004–1-1, SOD Assay Kit A001–3-2, POD Assay Kit A084–3 and CAT Assay Kit A007–1-1 (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) with ...

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  • US Patent Application for METHOD FOR PRODUCING

    A cell having reduced enzymatic activity of an enzyme can be identified using any method known in the art. For example, enzyme activity assays can be used to identify cells having reduced enzyme activity. See, for example, Enzyme Nomenclature, Academic Press, Inc., New York 2007.

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  • Regulation of the RNA and DNA nuclease activities

    2021-2-21 · 4422 NucleicAcidsResearch,2020,Vol.48,No.8 can be found in Supplemental Table S3. Synthetic target RNA was 5-end labeled using -32P-ATP, gel purified, eluted ...

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  • University of Groningen Mutational impact of classical ...

    Staphylococcus pyogenes using the method described previously (Pohl et al., 2016). Next, a Scoring algorithm (Doench et al., 2016) was used for calculat-ing the impact of nucleotides at certain positions, and then several protospac-er candidates with the highest scores were chosen for further sgRNA design.

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  • Identification of RipAZ1 as an avirulence determinant

    2021-1-3 · S. americanum plants were infected with R. solanacearum using the soil-drenching method and wilt symptoms were observed at 14 days postinfection (dpi). Among these interactions, we found that R. solanacearum strains Pe_26 and To_1 showed the most reproducible and robust avirulence and virulence phenotypes, respectively, in S. americanum ...

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  • Generation and Use of Recombinant Galectins - Wu -

    2021-3-3 · Dissolve 2.5 g kanamycin sulfate from Streptomyces kanamyceticus (MilliporeSigma, K1377) in 50 ml sterilized water. Pass through a 0.22-µm filter and aliquot into 1.5-ml vials and store at −20°C for up to 6 months. LB broth. Dissolve 20 mg of LB broth powder (RPI Research Products International, L24060) in 900 ml H 2 O.

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  • Genome-wide identification and functional analysis

    2021-2-2 · Relative expression was calculated using the 2 – CT method. The activities of the main disease-resistance enzymes, including ROS, SOD, POD, and CAT, were determined using the ROS Assay Kit E004–1-1, SOD Assay Kit A001–3-2, POD Assay Kit A084–3 and CAT Assay Kit A007–1-1 (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) with ...

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  • Identification of RipAZ1 as an avirulence

    2021-1-3 · S. americanum plants were infected with R. solanacearum using the soil-drenching method and wilt symptoms were observed at 14 days postinfection (dpi). Among these interactions, we found that R. solanacearum strains Pe_26 and To_1 showed the most reproducible and robust avirulence and virulence phenotypes, respectively, in S. americanum ...

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  • Development/Plasticity/Repair ...

    2014-9-19 · by daily systemic kanamycin injections (400 mg/kg) from P8 to P16, taking advantage of the particular sensitivity to aminoglycoside at this age (Carlier and Pujol, 1980). As we have reported previously (Alam et al., 2007), this method reliably eliminates IHCs in rats. In the present study, loss of hair cells was further verified by qPCR assay ...

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  • Late Blight resistant Potatoes by Cisgene Stacking

    2017-9-12 · Construct containing both cisgenic late blight R genes (Rpi-vnt1.1 and Rpi-sto1), but lacking the bacterial kanamycin resistance selection marker (NPTII) was transformed to the three selected potato varieties using Agrobacterium-mediated transformation. Rooted shoots were analysed by PCR for gene transfer products (desired R genes)

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  • Generation and Use of Recombinant Galectins - Wu

    2021-3-3 · Dissolve 2.5 g kanamycin sulfate from Streptomyces kanamyceticus (MilliporeSigma, K1377) in 50 ml sterilized water. Pass through a 0.22-µm filter and aliquot into 1.5-ml vials and store at −20°C for up to 6 months. LB broth. Dissolve 20 mg of LB broth powder (RPI Research Products International, L24060) in 900 ml H 2 O.

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  • WO 2015/161346 A1 - Agents And Methods For

    2015-10-29 · 5. The method of Claim 1 or 2 wherein the defensin variant comprises a Loop IB from a Class I defensin replacing the corresponding Loop IB from a Solanaceous Class II defensin. 6. The method of Claim 6 wherein the defensin variant is selected from the list consisting of HXP4, HXP34, HXP35, HXP37, HXP58, HXP72, HXP91, HXP92, HXP95 and HXP107. 7.

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  • Regulation of the RNA and DNA nuclease activities

    2021-2-21 · 4422 NucleicAcidsResearch,2020,Vol.48,No.8 can be found in Supplemental Table S3. Synthetic target RNA was 5-end labeled using -32P-ATP, gel purified, eluted ...

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  • THE JAPS

    Rpi-blb3 was obtained from S. bulbocastanum. The different R genes developed different level of resistances in the transformed potatoes than the non-transformed ones with those transformed with gene stacking of multiple R genes performed the best, followed by Rpi-vnt1.1 and Rpi-sto1 events. The Rpi-blb2 gene isolated from S.

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  • Comparative secretome analysis of different smut

    2019-8-16 · Tilletia horrida is a basidiomycete fungus that causes rice kernel smut, one of the most important rice diseases in hybrid rice growing areas worldwide. However, little is known about its mechanisms of pathogenicity. We previously reported the genome of T. horrida, and 597 genes that encoded secreted proteins were annotated. Among these were some important effector genes related …

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  • Indole-Induced Activities of β-Lactamase and Efflux

    2017-3-14 · performed using the biparental filter mating method. b-Lactamase Activity Assay b-lactamase activity was measured by hydrolysis of nitrocefin (O’Callaghan et al.,1972;Cavallari et al.,2013;Liu et al., 2016). Cells were grown to the exponential phase at 30 C with aeration. The cells were then treated with or without Amp or indole for 3 h.

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  • Genome-wide identification and functional analysis of

    2021-2-2 · Relative expression was calculated using the 2 – CT method. The activities of the main disease-resistance enzymes, including ROS, SOD, POD, and CAT, were determined using the ROS Assay Kit E004–1-1, SOD Assay Kit A001–3-2, POD Assay Kit A084–3 and CAT Assay Kit A007–1-1 (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) with ...

    Get Price
  • Identification of RipAZ1 as an avirulence determinant

    2021-1-3 · S. americanum plants were infected with R. solanacearum using the soil-drenching method and wilt symptoms were observed at 14 days postinfection (dpi). Among these interactions, we found that R. solanacearum strains Pe_26 and To_1 showed the most reproducible and robust avirulence and virulence phenotypes, respectively, in S. americanum ...

    Get Price
  • Structural analysis of a putative aminoglycoside N ...

    2011-7-15 · Cells were induced with 0.4 mM IPTG for 30 min., then approximately 10 9 cells were spotted on agar plates containing LB Broth Lennox (RPI) with 100 µg/ml ampicillin, 34 µg/ml chloramphenicol and 0.5 mM IPTG. Then Antimicrobial Susceptibility Test discs (BD BBL™), containing 30 µg of amikacin, 30 µg of neomycin, 30 µg of kanamycin, 10 ...

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  • Intercalation activating fluorescence DNA probe and its ...

    2009-3-5 · method of fluorescence real-time monitoring of iso-thermal RNA sequence amplification (the ‘‘TRC’’ re-action) using the INAF DNA probe. We examined the fundamental performance of the present method and found that the method enabled ultrarapid and direct quantification of pathogenic gene expression with high sensitivity.

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  • THE JAPS

    Rpi-blb3 was obtained from S. bulbocastanum. The different R genes developed different level of resistances in the transformed potatoes than the non-transformed ones with those transformed with gene stacking of multiple R genes performed the best, followed by Rpi-vnt1.1 and Rpi-sto1 events. The Rpi-blb2 gene isolated from S.

    Get Price
  • WO 2015/161346 A1 - Agents And Methods For

    2015-10-29 · 5. The method of Claim 1 or 2 wherein the defensin variant comprises a Loop IB from a Class I defensin replacing the corresponding Loop IB from a Solanaceous Class II defensin. 6. The method of Claim 6 wherein the defensin variant is selected from the list consisting of HXP4, HXP34, HXP35, HXP37, HXP58, HXP72, HXP91, HXP92, HXP95 and HXP107. 7.

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  • IJPSR, 2012; Vol. 3(3): Gracelin 868 -73 ISSN: 0975 8232

    Concentration) and RPI (Relative Percentage Inhibition) values, Angiopteris evecta could be used as potential plant for the management of pathogenic bacteria, X. c. pv. c which is known to cause diseases on many vegetables and Centella asiatica. INTRODUCTION: A bacterial leaf spot disease caused by Xanthomonas campestris pv. centellae (X. c. pv.

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  • Indole-Induced Activities of β-Lactamase and Efflux Pump ...

    2017-4-13 · performed using the biparental filter mating method. b-Lactamase Activity Assay b-lactamase activity was measured by hydrolysis of nitrocefin (O’Callaghan et al.,1972;Cavallari et al.,2013;Liu et al., 2016). Cells were grown to the exponential phase at 30 C with aeration. The cells were then treated with or without Amp or indole for 3 h.

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  • 5-phosphate | Sigma-Aldrich

    Summary: The protein encoded by this gene is an enzyme, which catalyzes the reversible conversion between ribose-5-phosphate and ribulose-5-phosphate in the pentose-phosphate pathway. This gene is highly conserved in most organisms. The enzyme plays an essential role in the carbohydrate metabolism. Mutations in this gene cause ribose 5 ...

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  • Frontiers | Characterization of CRN-Like Genes From ...

    Grapevine downy mildew is an insurmountable disease that endangers grapevine production and the wine industry worldwide. The causal agent of the disease is the obligate biotrophic oomycete Plasmopara viticola, for which the pathogenic mechanism remains largely unknown. Crinkling and necrosis proteins (CRN) are an ancient class of effectors utilized by pathogens, including oomycetes, that ...

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  • Postnatal Expression of Neurotrophic Factors

    2015-10-29 · 5. The method of Claim 1 or 2 wherein the defensin variant comprises a Loop IB from a Class I defensin replacing the corresponding Loop IB from a Solanaceous Class II defensin. 6. The method of Claim 6 wherein the defensin variant is selected from the list consisting of HXP4, HXP34, HXP35, HXP37, HXP58, HXP72, HXP91, HXP92, HXP95 and HXP107. 7.

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  • WO 2015/161346 A1 - Agents And Methods For

    In the Rpi-blb1 coinfiltrations, the HR was observed with the two PexRD6/IpiO clones starting at 4 DAI, and for Rpi-blb2, the HR was observed with both PexRD39 and PexRD40 constructs starting at 3 DAI (Figures 5B to 5D). Altogether, these experiments indicate that the identified clones are specifically recognized by the cognate R genes.

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  • In Planta Expression Screens of Phytophthora

    2017-5-3 · -Either selected using a positive screen (based on resistance to the antibiotic kanamycin) and induced to regenerate a whole plant. -Or induced to regenerate a whole plant without using a positive screen. This is done in case of the single gene Rpi-vnt1 construct, that does not harbour a selection marker. All regenerated

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  • Summary Notification Information Format

    2021-2-21 · 4422 NucleicAcidsResearch,2020,Vol.48,No.8 can be found in Supplemental Table S3. Synthetic target RNA was 5-end labeled using -32P-ATP, gel purified, eluted ...

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  • Regulation of the RNA and DNA nuclease activities

    2008-11-11 · In this study, we describe the use of nuclease-resistant molecular beacons (MBs) for the real-time detection of coxsackievirus B6 replication in living Buffalo green monkey kidney (BGMK) cells via Tat peptide delivery. A nuclease-resistant MB containing 2′- O -methyl RNA bases with phosphorothioate internucleotide linkages was designed to specifically target an 18-bp 5′ noncoding …

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  • Visualizing the dynamics of viral replication in living ...

    Endo-β-mannanase (EC [3.2.1.78][1]) is involved in cell wall disassembly and the weakening of plant tissues by degrading mannan polymers in the cell walls. Endo-β-mannanase genes are expressed in tomato ( Lycopersicon esculentum ) seeds ( LeMAN1 and LeMAN2 ) and fruits ( LeMAN3 and LeMAN4 ). A novel endo-β-mannanase gene (termed LeMAN5 ) was found in the tomato genome by genome …

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  • A Novel Endo-β-Mannanase Gene in Tomato

    2014-11-18 · Thus, cellophane membrane was a valid model for the cyst germination. The in vitro inoculation method could be applied to other similar plant-pathogen interaction studies. Using the CEGMA tool, 243 (98%) of the 248 conserved single-copy eukaryotic genes mapped against the transcriptome assembly were identified in our study.

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  • Transcriptomic analysis of the phytopathogenic

    2021-2-19 · The evolutionary distances were computed using the Poisson correction method (Zuckerkandl and Pauling 1965) and are in the units of the number of amino acid substitutions per site. The analysis involved 17 amino acid sequences. All positions containing gaps and missing data were eliminated. There were a total of 108 positions in the final dataset.

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