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qizhenzengmiankeli granules for pseudorabies using method

  • Pseudorabies virus infection inhibits stress granules ...

    2020-8-1 · Pseudorabies virus (PRV) is one of the most notorious pathogens in the global pig industry. During infection, viruses may evolve various strategies, such as modulating stress granules (SGs) formation, to create an optimal surroundings for viral replication. However, the interplay between PRV infection and SGs formation remains largely unknown.

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  • Function of Torsin AAA+ ATPases in Pseudorabies

    Cells were transfected with 1 µg of plasmid DNA using polyethylenimine (PEI) , and harvested 24 h post transfection by scraping into the medium, pelleted, washed twice with phosphate-buffered saline (PBS) and lysed in SDS-containing sample buffer (0.13 M Tris-HCl, pH 6.8; 4% SDS; 20% glycerin; 0.01% bromophenol blue; 10% 2-mercaptoethanol). Here we used PEI transfection, instead of calcium phosphate-precipitation method…

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  • Golgi staining-like retrograde labeling of brain

    2020-10-1 · The gold standard to detect the fine details of neuronal dendritic arborization is still the silver staining technique that has been used by Cajal, called Golgi metallic impregnation method. This method or its updates (Rapid Golgi staining, Golgi-Cox method) allows staining a limited number of neurons in their entirety, in a still unexplained random manner.

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  • Quantitative electron microscope studies on the

    The appearance of the fine-grained aggre- gates appears to be related to the growth of virus in the nuclei. The aggregates resemble those found in cells infected with other viruses including varicella (Tournier et al., 1957), polyoma (Bernhard et al., 1959), EMC (Hinz et al., 1962), SV40 (Gran- boulan et al., 1963), and pseudorabies (Felluga, 1963).

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  • Progress and Perspective of Antiviral Protective

    2020-6-23 · The average particle size of Ag NPs is an important factor that affects its antiviral ability. For example, Krzyzowska et al. report the synthesis of Ag NPs with an average particle size of 33 nm, prepared by chemical reduction method, using tannic acid as surface ligands. This Ag NPs couls effectively control the herpes simplex virus type 2 (HSV-2) infection in mice by inhibiting the adhesion …

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  • Detection of Prion Protein in Formalin-Fixed Brain by ...

    2018-2-23 · ing. 1 This novel method of antigen enhancement, termed hydrated autoclaving, was originally devised to improve the immunoreactivity of certain brain proteins. 10 Hydrated au-toclaving has not been used to detect other antigens. How-ever, antigen enhancement is believed to result from heat denaturation of the protein, and the same mechanism has

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  • A soluble form of human nectin-2 impairs exocrine ...

    2016-3-1 · Transgenic mice expressing hNectin-2Ig were generated as described previously , by microinjection method of the transgenic fragment containing the CAG promoter (cytomegalovirus IE enhancer and chicken ß-actin promoter), the hNectin-2Ig gene, and the rabbit ß-globin polyA signal. To identify transgenic founders, genomic DNA was prepared by using the FTA elute micro cards (Whatman) and PCR was performed using …

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  • The development of antibodies in rabbits and cattle ...

    1977-6-1 · The reproducibility of the micro-well method was investigated in 27 successive titrations of scrim from rabbit 54; the .mean titre was 1 : 3 430~ with a range of 1 : 512 to 1 : 4 096. The development of IIF and VN antibodies to MCFV during the course of infection in rabbits The development of both IIF and VN antibodies to MCFV during the course of infection in rabbits is shown graphically in Fig 3.

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  • Efficient Retrograde Transport of Pseudorabies Virus ...

    After replicating in epithelial cells, alphaherpesviruses such as pseudorabies virus (PRV) invade axons of peripheral nervous system neurons and undergo retrograde transport toward the distant cell bodies. Although several viral proteins engage molecular motors to facilitate transport, the initial steps and neuronal responses to infection are poorly understood. Using compartmented neuron ...

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  • Function of Torsin AAA+ ATPases in Pseudorabies

    Newly assembled herpesvirus nucleocapsids traverse the intact nuclear envelope by a vesicle-mediated nucleo-cytoplasmic transport for final virion maturation in the cytoplasm. For this, they bud at the inner nuclear membrane resulting in primary enveloped particles in the perinuclear space (PNS) followed by fusion of the primary envelope with the outer nuclear membrane (ONM).

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  • Ex vivo infection of human embryonic spinal cord

    2010-5-29 · Genetically modified pseudorabies virus (Prv) proved suitable for the delivery of foreign genes to rodent embryonic neurons ex vivo and maintaining foreign gene expression after transplantation into spinal cord in our earlier study. The question arose of whether human embryonic neurons, which are known to be more resistant to Prv, could also be infected with a mutant Prv.

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  • Quantitative electron microscope studies on the

    VIROLOGY 24, 523-538 (1964) Quantitative Electron Microscope Studies on the Growth of Herpes Virus Using the Techniques of Negative Staining and Ultramicrotomy D. H. WATSON,1 P. WILDY,1 AND W. C. RUSSELL2 Institute of Virology, The University, Glasgow, W.SS., Scotland Accepted July 9, 1964 The morphological differentiation of herpes virus during a single-step growth cycle has been studied by ...

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  • A soluble form of human nectin-2 impairs exocrine ...

    2016-3-1 · 1. Introduction. Nectins are Ca 2+-independent immunoglobulin (Ig)-like cell–cell adhesion molecules .The nectin family is composed of four members, nectin-1, -2, -3 and -4, all of which, except nectin-4, have two or three splicing variants: nectin-1α, -1β, -1γ, -2α, -2δ, -3α, -3β, and -3γ .Nectin members, except nectin-1γ, consist of an extracellular region with three Ig-like loops ...

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  • Progress and Perspective of Antiviral Protective

    2020-6-23 · Public health events caused by viruses pose a significant risk to humans worldwide. From December 2019 till now, the rampant novel 2019 coronavirus (SAR-CoV-2) has hugely impacted China and over world. Regarding a commendable means of protection, mask technology is relatively mature, though most of the masks cannot effectively resist the viral infections. The key material of the mask is …

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  • Nucleic Acids and Proteins and Methods for Making

    A method for whole cell engineering of new or modified phenotypes by using real-time metabolic flux analysis, the method comprising the following steps: (a) making a modified cell by modifying the genetic composition of a cell, wherein the genetic composition is modified by addition to the cell of a nucleic acid comprising a sequence as set ...

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  • HYDROLASES, NUCLEIC ACIDS ENCODING THEM

    2011-11-10 · In one aspect of this second method, step (d) further comprises using ion exchange resins. The kinetically controlled conditions can comprise non-equilibrium conditions resulting in production of an end product having greater than a 2:1 ratio of 1,3-DAG to 2,3-DAG. The composition of step (b) can comprise a fluorogenic fatty acid (FA).

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  • CELLULOLYTIC ENZYMES, NUCLEIC ACIDS

    A method for utilizing a cellulase as a nutritional supplement in an animal diet, the method comprising: preparing a nutritional supplement containing a cellulase enzyme comprising at least thirty contiguous amino acids of a polypeptide as set forth in claim 48, or a polypeptide encoded by a nucleic acid as set forth in claim 1; or a ...

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  • CRISPR/Cas Technology in Pig-to-Human

    2021-3-21 · 5. Conclusions. The use of the CRISPR/Cas system in genetic engineering has enabled the enormous development of pig-to-human xenotransplantation research. Thanks to this technology, animals with multi-modifications were obtained, enabling the reduction in the primates’ immune reaction to a porcine xenograft.

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  • Cloning, expression and characterization of gE protein

    2010-6-8 · The gE protein of duck plague virus is the important membrane glycoprotein, its protein characterization has not been reported. In this study, we expressed and presented the characterization of the DPV gE product. According to the sequence of the gE gene, a pair of primers were designed, and the DNA product with 1490bp in size was amplified by using the polymerase chain reaction (PCR).

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  • Nanotechnology - IOPscience

    2021-5-28 · Vol 2, 1991. Vol 1, 1990. Focus issues. Focus on Topological Science and Technologies in Nano-Structures Focus on 2D Materials for Microelectronic Devices and Nanoscale Heat Dissipation Focus on 3D Micro/Nano Structures Based on Atomically Thin 2D Materials Focus on Advances in Eco-Friendly Nanomaterials and Nanotechnologies: Design, Processing ...

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  • CVI Table of Contents Volume 10, Issue 4

    2020-12-18 · The release of a biological weapon (BW) agent by a terrorist group or military force would likely be silent and undetectable or nearly so. As shown by anthrax attack during the fall of 2001 in the eastern United States, patients would begin appearing at hospitals and clinics within several days of exposure, most presenting with nonspecific flu-like symptoms.

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  • Granular polymer composite and production process

    2020-3-15 · Wang Tian, Xiang Yuqiang, Liu Xiaoxiao, Chen Wenli, Hu Yonggang*, A novel fluorimetric method for laccase activities measurement using Amplex Red as substrate, Talanta, 2017,162:143-150. 108. Nie Hailing, Xiao Yujie, Liu Huizhong, He Jinzhi, Chen Wenli*, Huang Qiaoyun*, FleN and FleQ play a synergistic role in regulating lapA and bcs operons in Pseudomonas putida KT2440, Env Microbiol …

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  • Richard Edwin Shope | Biographical Memoirs: Volume

    Using the above-mentioned production process, the formation of a granular polymer composite in which the composite granules have an average granule diameter of 1.2 to 30.0 microns in the range of grain size distribution of d 75 /d 25 ≤2, density of 1.05 to 1.35 g/cm 3 and pore size of 500 to 1000 angstroms and also a coating of the calcium ...

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  • Nanomaterials for Airborne Virus Inactivation: A Short ...

    2020-3-15 · 1. Lin Da#, Hong Ping#, Zhang Siheng, Xu Weize, Jamal M, Yan Keji, Lei Yingying, Li Liang, Ruan Yijun, Fu Zhen F., Li Guoliang*, Cao Gang*, Digestion-ligation-only Hi-C is an efficient and cost-effective method for chromosome conformation capture ...

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  • The pseudorabies virus vhs protein cleaves RNA

    2016-1-7 · The virion host shutoff protein (vhs), encoded by the gene UL41, has RNase activity and is the key regulator of the early host shutoff response induced by type 1 herpes simplex virus.Despite low amino acid similarity, the vhs protein of the swine herpesvirus, pseudorabies …

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  • The pH of the Secretory Pathway: Measurement,

    2019-10-29 · The luminal pH of the secretory pathway plays a critical role in the posttranslational modification and sorting of proteins and lipids. The pH of each one of the organelles that constitute the pathway is unique, becoming more acidic as the biosynthetic cargo approaches its destination. The methods used for measurement of pH in the secretory ...

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  • Simultaneous visualization of extrinsic and intrinsic

    2014-9-17 · Using this method, we successfully labeled the corticothalamic (CT) cells of the mouse somatosensory barrel field (S1BF) and motor cortex (M1) in their entirety. We also labeled contra- and ipsilaterally-projecting corticocortical (CC) cells of M1 by targeting contralateral M1 or ipsilateral S1 for retrograde infection.

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  • Detection of Prion Protein in Formalin-Fixed Brain by ...

    2018-2-23 · processed by the hydrated autoclaving method. A staining reaction was observed in only 1 sheep from the scrapie-free flock, and in that animal, the immunostained material was limited to sparse, small, intracytoplasmic granules that were present in just a few neurons. Based on this observation, tissues were considered positive for PrP-res only ...

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  • blue to a PH 5, I have been able to - JSTOR

    stain. Red staining granules in the cytoplasm of nerve cells were first described by Held,13 in 1895, by his erythrosin (iodo-eosin) methylene blue method. He termed them neurosomes. I have obtained satisfactory results from using erythrosin to stain Negri bodies. The Negri body gives a …

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  • CPC Scheme - C07K PEPTIDES

    C07K 14/70539. In this subclass non-limiting references (in the sense of paragraph 39 of the Guide to the IPC) may still be displayed in the scheme. C07K 1/00. General methods for the preparation of peptides {, i.e. processes for the organic chemical preparation of peptides or proteins of any length} [2016-08]

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  • Efficacy of enteric‐coated tilmicosin granules in pigs ...

    2019-10-7 · Background. Porcine infectious pleuropneumonia caused by Actinobacillus pleuropneumoniae (App) is one of the most serious infectious diseases in pigs and has brought huge economic losses to the world pig industry. The aim of this trial was to evaluate the effect of enteric-coated tilmicosin granule in the treatment and control of artificial infection of App.

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  • Proceedings of the Food and Drug Administration

    2019-5-29 · On November 29, 2018, experts in the field of infectious diseases, pathogen reduction technologies (PRTs) and other participants from blood centers, academia, and industry gathered at the Food and Drug Administration (FDA) White Oak Campus in Silver Spring, Maryland, for a 2-day public workshop entitled “Pathogen Reduction Technologies for Blood Safety.”

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  • Yingjuan Qian - Publications

    2021. Sun L, Chen H, Ming X, Bo Z, Shin HJ, Jung YS, Qian Y. Porcine Epidemic Diarrhea Virus Infection Induces Caspase-8 mediated G3BP1 Cleavage and Subverts Stress Granules to Promote Viral Replication. Journal of Virology. PMID 33568512 DOI: 10.1128/JVI.02344-20.

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  • Biosensors | Free Full-Text | Nanobioengineered

    Recent advancement has been accomplished in the field of biosensors through the modification of cellulose as a nano-engineered matrix material. To date, various techniques have been reported to develop cellulose-based matrices for fabricating different types of biosensors. Trends of involving cellulosic materials in paper-based multiplexing devices and microfluidic analytical technologies have ...

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  • Directional Transneuronal Infection by Pseudorabies

    Pseudorabies virus (PRV) is a member of the neurotropic Alphaherpesvirus subfamily which also includes the human pathogens herpes simplex virus types 1 and 2 (HSV-1 and -2) and varicella-zoster virus (VZV) and the animal pathogens bovine herpesvirus type I (BHV-1), equine herpesvirus type I (EHV-1), and feline herpesvirus type I (FHV-1). PRV establishes a latent infection in its natural host ...

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  • CDC - Diagnosis: Histologic Examination - Rabies

    Pseudorabies virus injections. Approximately 104 h (4.75 days) prior to euthanasia, unilateral injections of the attenuated Bartha strain (6×10 8 pfu/mL) of PRV (courtesy of Dr. L.W. Enquist) were made into the ventral head of the bulbospongiosus muscle (BSM). We found in our pilot studies, as have others, that the attenuated Bartha strain infects a few medullary neurons as early as 4 days ...

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  • Bilateral Bulbospinal Projections to Pudendal

    2021-3-21 · 5. Conclusions. The use of the CRISPR/Cas system in genetic engineering has enabled the enormous development of pig-to-human xenotransplantation research. Thanks to this technology, animals with multi-modifications were obtained, enabling the reduction in the primates’ immune reaction to a porcine xenograft.

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  • CRISPR/Cas Technology in Pig-to-Human

    2018-2-23 · processed by the hydrated autoclaving method. A staining reaction was observed in only 1 sheep from the scrapie-free flock, and in that animal, the immunostained material was limited to sparse, small, intracytoplasmic granules that were present in just a few neurons. Based on this observation, tissues were considered positive for PrP-res only ...

    Get Price
  • Detection of Prion Protein in Formalin-Fixed Brain by ...

    2020-12-11 · Heat observation was preliminarily performed twice a day from the age of 6 months using the method of behaviour observation for sow oestrus (‘standing’ to boars and back pressure). A heat cycle of 21 days was divided into four stages of pro-oestrus, oestrus, metoestrus and dioestrus according to the definitions described by Senger ( 2003 ).

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  • Identification of the gut microbiota biomarkers

    Using the above-mentioned production process, the formation of a granular polymer composite in which the composite granules have an average granule diameter of 1.2 to 30.0 microns in the range of grain size distribution of d 75 /d 25 ≤2, density of 1.05 to 1.35 g/cm 3 and pore size of 500 to 1000 angstroms and also a coating of the calcium ...

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  • Granular polymer composite and production process

    The main surgical techniques were penetrating keratoplasty (PK) (19,896, 54.34%), anterior lamellar keratoplasty (ALK) (13,869, 37.88%). The proportion of PK decreased from 57.97% in 2014 to 52.88% in 2018 while the proportion of ALK increased from 36.04% in 2014 to 37.92% in 2018. The geographical distribution of keratoplasties performed in ...

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  • FLOSEAL Hemostatic Matrix - Baxter - PDF Catalogs ...

    FLOSEAL Matrix is the combination of the Gelatin Matrix component and the reconstituted Thrombin (Human) component. Thrombin must be added to the Gelatin Matrix component prior to use. FLOSEAL Matrix is biocompatible and resorbed within 6 to 8 weeks, consistent with normal wound healing. FLOSEAL Matrix is intended only for topical administration.

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  • Experimental Animals - JST

    A method of preparation of template DNA for PCR was established using the lysis buffer (1 × PCR reaction buffer supplemented with proteinase K at a concentration of 40 μg/ml) developed by the authors.

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  • The role of oxytocin in regulation of appetitive

    However, using a much shorter period of exposure to this 60% HFD, 10 days, from PND 20 to PND 29 in male Sprague Dawley rats, significantly lower OXT levels (measured by ELISA) were found in the infralimbic medial prefrontal cortex, compared to chow-fed controls . Note that there were no significant BW differences between the two groups in this ...

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  • Cellular mechanisms of alpha herpesvirus egress: live

    2014-12-4 · Introduction. Pseudorabies virus (PRV; suid herpesvirus 1) is a veterinary pathogen, widely used as a neuroanatomical tracing tool, and related to the human alpha herpesviruses varicella-zoster virus (VZV) and herpes simplex virus 1 and 2 (HSV-1 & -2).

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  • Molecular pathways linking adipose innervation to

    Dual pseudorabies virus tracing of ... using a whole-tissue clearing method that permits visualization of fluorescently labelled ... NPY is located in cytosolic granules in the terminals of ...

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  • WO 2009/020459 A2 - Glucanases, Nucleic Acids

    The main surgical techniques were penetrating keratoplasty (PK) (19,896, 54.34%), anterior lamellar keratoplasty (ALK) (13,869, 37.88%). The proportion of PK decreased from 57.97% in 2014 to 52.88% in 2018 while the proportion of ALK increased from 36.04% in 2014 to 37.92% in 2018. The geographical distribution of keratoplasties performed in ...

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  • Frontiers | Expression Pattern Analysis of Antiviral

    In another embodiment, the invention provides methods for drilling or oil and gas well washing and/or a fracturing method using mixtures ('cocktails') of enzymes; and in one aspect, the mixture ('cocktail') comprises polymer-degrading enzymes, and optionally at least one polymer-degrading enzyme is a lignin degrading enzyme, a lignin peroxidase ...

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  • Emerging translation strategies during virus–host ...

    2020-8-5 · Using the ribopuromycylation method to visualize subcellular translation activity, it has been reported that the virus factory of vaccinia virus and reovirus possess elevated translation activity compared to the surrounding cytoplasm (David et al., 2011; Desmet, Anguish, & Parker, 2014).

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  • Production of Recombinant Porcine Interferon alpha

    2010-10-20 · Interferons (IFNs) are involved in the pathogenesis and recovery of viral and other infectious diseases. Recombinant IFNs have been used as anti-infectious agents exhibiting a broad range of antiviral and immunomodulatory properties in both human and domestic animals. In this report, we describe a highly efficient and economical approach to purify porcine IFN alpha (PoIFNα) using ...

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  • Erythrocytenmorphologische

    Hino, S., and N. Furusawa: On increasing the speed of hematological tests with special references to the hematocrit method with capillaries using a high-speed centrifuge. Clin. …

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  • Qunlei Jiang - Publications

    [Key physical parameters of hawthorn leaf granules by stepwise regression analysis method]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica. 42: 1894-1900. PMID 29090548 : 0.72: 2017: Shi X, Ba R, You H, Jiang Q, Huang J, Mao J, Han L, Zhang S, Zhuang Q, Yu X, Wang L, Wang Y, Li D, Zhu W, Zhang Y ...

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  • CDC - Diagnosis: Histologic Examination - Rabies

    2014-7-14 · The hand of the reconstruction was established by the tilt-pair method (Belnap et al, 1997; Cheng et al, 2002). For higher resolution, ~14,000 particles from Polara micrographs were reconstructed in similar fashion, using the CM200 EncA map filtered to 15 Å as initial reference.

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  • A virus capsid‐like nanocompartment that stores iron

    2016-5-23 · Duabanga grandiflora or known in Malaysia as Berembang Bukit, Megawasih, or Pedada Bukit, is a native plant of the Southeast Asian countries. In this study, the anti-viral properties of D. grandiflora were investigated. The D. grandiflora leaf extracts were obtained with ethyl acetate, hexane, and ethanol as solvents and labelled 37 leaf ethyl acetate (37 L EA), 37 leaf hexane (37 L H), 37 ...

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  • Antiviral and virucidal activities of Duabanga

    2020-4-28 · It has been known for a few decades that transcripts can be marked by dozens of different modifications. Yet, we are just at the beginning of charting these marks and understanding their functional impact. High-quality methods were developed for the profiling of some of these marks, and approaches to finely study their impact on specific phases of the RNA life-cycle are available, including ...

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  • Frontiers | Direct RNA Sequencing for the Study of ...

    In another embodiment, the invention provides methods for drilling or oil and gas well washing and/or a fracturing method using mixtures ('cocktails') of enzymes; and in one aspect, the mixture ('cocktail') comprises polymer-degrading enzymes, and optionally at least one polymer-degrading enzyme is a lignin degrading enzyme, a lignin peroxidase ...

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  • Epi4Dogs | Enzymes in Brief | Managing EPI | Non

    FLOSEAL Matrix is the combination of the Gelatin Matrix component and the reconstituted Thrombin (Human) component. Thrombin must be added to the Gelatin Matrix component prior to use. FLOSEAL Matrix is biocompatible and resorbed within 6 to 8 weeks, consistent with normal wound healing. FLOSEAL Matrix is intended only for topical administration.

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  • WO 2009/020459 A2 - Glucanases, Nucleic Acids

    Journal of Cancer Research and Therapeutics. 14: S767-S773. PMID 30249901 DOI: 10.4103/0973-1482.187304. Zhou L, Sheng D, Deng Q, Wang D, Liu S. Development of a novel method for rapid cloning of shRNA vectors, which successfully knocked down CD44 in …

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  • FLOSEAL Hemostatic Matrix - Baxter - PDF Catalogs ...

    2020-9-14 · 北海道におけるアイガモの寄生蠕虫類調査, 吉野 智生, 遠藤 大二, 大沼 学, 長 雄一, 斉藤 美加, 桑名 貴, 浅川 満彦, 獣医疫学雑誌, 獣医疫学雑誌, 15, (2) 106 - 109, 2011年, 査読有り. DNA damage of blood lymphocytes and neutrophils in cattle with lymphosarcoma, T. Mohamed, D. Endoh, S. Oikawa ...

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  • Lei Zhou - Publications

    A platelet estimate that is performed with the use of a differential blood cell film requires the counting of platelets in a minimum of ____ microscopic fields. 10. Multiplying the platelet estimate obtained from the blood film by ____ to get the platelet estimate per µL of blood. 20,000. Lead poisoning can cause.

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  • Molecular pathways linking adipose innervation to

    Dual pseudorabies virus tracing of ... using a whole-tissue clearing method that permits visualization of fluorescently labelled ... NPY is located in cytosolic granules in the terminals of ...

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  • WO 2009/020459 A2 - Glucanases, Nucleic Acids

    In another embodiment, the invention provides methods for drilling or oil and gas well washing and/or a fracturing method using mixtures ('cocktails') of enzymes; and in one aspect, the mixture ('cocktail') comprises polymer-degrading enzymes, and optionally at least one polymer-degrading enzyme is a lignin degrading enzyme, a lignin peroxidase ...

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  • Frontiers | Inhibition of Cathepsins B Induces ...

    TRANSFERASES AND OXIDOREDUCTASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING. THEM. REFERENCE TO SEQUENCE LISTING SUBMITTED VIA EFS-WEB This application was filed electronically via the USPTO EFS-WEB server, as authorized and set forth in MPEP §1730 II.B.2.(a)(A), and this electronic filing includes an electronically submitted …

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  • WO 2009/088949 A1 - Transferases And

    2008-2-15 · Full Text PDF [401K] Ten calves harboring microfilariae (Mf) were injected with ivermectin at a single dose of 200 μg/kg body weight. Thereafter, the number of calves harboring Mf was gradually reduced to 7, 2 and 0 by 1, 4 and 12 weeks, respectively and remained negative for microfilariae by 16 weeks after injection.

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  • Journal of Veterinary Medical Science

    A method of preparation of template DNA for PCR was established using the lysis buffer (1 × PCR reaction buffer supplemented with proteinase K at a concentration of 40 μg/ml) developed by the authors.

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  • Frontiers | Exogenous Fecal Microbiota

    Many viruses that are pathogenic for humans, including HIV-1, herpes simplex virus and measles, can disseminate by moving directly from cell to cell. Quentin Sattentau discusses the mechanisms of ...

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  • Experimental Animals - JST

    [Key physical parameters of hawthorn leaf granules by stepwise regression analysis method]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica. 42: 1894-1900. PMID 29090548 : 0.72: 2017: Shi X, Ba R, You H, Jiang Q, Huang J, Mao J, Han L, Zhang S, Zhuang Q, Yu X, Wang L, Wang Y, Li D, Zhu W, Zhang Y ...

    Get Price
  • A virus capsid‐like nanocompartment that stores iron

    2014-7-14 · The hand of the reconstruction was established by the tilt-pair method (Belnap et al, 1997; Cheng et al, 2002). For higher resolution, ~14,000 particles from Polara micrographs were reconstructed in similar fashion, using the CM200 EncA map filtered to 15 Å as initial reference.

    Get Price
  • FLOSEAL Hemostatic Matrix - Baxter - PDF Catalogs ...

    FLOSEAL Matrix is the combination of the Gelatin Matrix component and the reconstituted Thrombin (Human) component. Thrombin must be added to the Gelatin Matrix component prior to use. FLOSEAL Matrix is biocompatible and resorbed within 6 to 8 weeks, consistent with normal wound healing. FLOSEAL Matrix is intended only for topical administration.

    Get Price
  • STUDIES ON AN UNCOMPLICATED CORYZA OF THE

    The method employed was essentially that of Li and Rivers; using, however, test tubes instead of flasks. Traub (5) had successfullyemployed tubes in the cultivation of pseudorabies virus as had also Dochez, Mills, and Kneeland (6) in the case of the common cold virus of man.

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  • Class 424: DRUG, BIO-AFFECTING AND BODY

    2000-6-30 · 523, Synthetic Resins or Natural Rubbers, 105 for nonmedicated compositions for contact with living animal tissue and especially subclass 111 for compositions used to protect wounds, lesions, etc. The mere method of using these compositions for contact with living animal tissue is classified either in Class 424 or Class 514.

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  • WO 2009/020459 A2 - Glucanases, Nucleic Acids

    In another embodiment, the invention provides methods for drilling or oil and gas well washing and/or a fracturing method using mixtures ('cocktails') of enzymes; and in one aspect, the mixture ('cocktail') comprises polymer-degrading enzymes, and optionally at least one polymer-degrading enzyme is a lignin degrading enzyme, a lignin peroxidase ...

    Get Price
  • Science Research Articles: Journal Papers | Clyto Access

    2015-11-17 · Herpesvirus of turkey (HVT) is an avian alphaherpesvirus used as live vaccine against Marek's disease for more than 40 years. Here, we describe the application of CRISPR/Cas9-based genome editing as a rapid and efficient method of introducing targeted mutations to the genome of a live herpesvirus vaccine vector using a transfection-infection ...

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  • Lei Zhou - Publications

    Journal of Cancer Research and Therapeutics. 14: S767-S773. PMID 30249901 DOI: 10.4103/0973-1482.187304. Zhou L, Sheng D, Deng Q, Wang D, Liu S. Development of a novel method for rapid cloning of shRNA vectors, which successfully knocked down CD44 in …

    Get Price
  • CATTLE - GEOCITIES.ws

    2011-4-3 · PSEUDORABIES. Rare, but Suid Herpesvirus 1 can be spread from the pig to cattle via nasal discharge in contact w abraded skin or nasal mucosa. Causes sudden death. Intense, local pruritis w violent licking, chewing and rubbing of the part

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  • Pathology 1&2 Flashcards - Questions and Answers |

    A platelet estimate that is performed with the use of a differential blood cell film requires the counting of platelets in a minimum of ____ microscopic fields. 10. Multiplying the platelet estimate obtained from the blood film by ____ to get the platelet estimate per µL of blood. 20,000. Lead poisoning can cause.

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  • PSC 101: Chapter 5 Flashcards | Quizlet

    PSC 101: Chapter 5. STUDY. PLAY. lesion. wound or injury - researcher destroys part of the brain (brain lesion) lesion studies. experiments in which part of the brain is damaged and the individual's behavior is subsequently observed. kainic acid. ex of excitatory amino acid, which kills neurons by …

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