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kanamycin sulfatekanamycin a monosulfate using method

  • Kanamycin monosulfate (Kanamycini monosulfas)

    2019-10-4 · Manufacture. Kanamycin monosulfate is produced by methods of manufacture designed to eliminate or minimize substances lowering blood pressure. The method of manufacture is validated to demonstrate that the product, if tested, would comply with the test as described under 3.7 Undue toxicity, using 0.5 mL of a solution containing 2 mg per mL.

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  • Final text for addition to The International Pharmacopoeia

    Kanamycin monosulfate is produced by methods of manufacture designed to eliminate or minimize substances lowering blood pressure. The method of manufacture is validated to demonstrate that the product, if tested, would comply with the test as described under 3.7 Undue toxicity, using 0.5 ml of a solution containing 2 mg per ml.

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  • Final text for addition to The International Pharmacopoeia

    Kanamycin acid sulfate is a form of kanamycin sulfate prepared by adding sulfuric acid to a solution of Kanamycin monosulfate or kanamycin and drying by a suitable method; Kanamycin acid sulfate, Kanamycin monosulfate and kanamycin are

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  • Kanamycin Monosulfate, USP Grade | GoldBio

    TESTED AGAINST BOTH SENSITIVE AND RESISTANT CELLS AT GOLD BIOTECHNOLOGY LAB. Product Specifications: Kanamycin Monosulfate. Kanamycin A. Formula: C 18 H 36 N 4 O 11 · H 2 SO 4. MW: 582.58 g/mol. Storage/Handling: Store desiccated at -20°C. Soluble in water.

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  • Kanamycin A Monosulfate | CAS 25389-94-0 | SCBT -

    Kanamycin A Monosulfate is an antibiotic that belongs to the family of aminoglycosides. The antibiotic associates with ribosomal RNA by forming a complex at the bacterial ribosomal A site.

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  • Kanamycin Monosulfate | CAS 25389-94-0 | Antibiotic ...

    Kanamycin is an antibiotic typically used against prokaryotes. It is an aminoglycoside that indirectly interrupts protein synthesis in non-resistant cells by binding to the ribosome and disrupting translocation of tRNA during translation. Aminoglycosides like kanamycin 'irreversibly' bind to specific 30S-subunit proteins and 16S rRNA.

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  • Kanamycin Sulfate - Thermo Fisher Scientific

    Kanamycin Sulfate is a water-soluble antibiotic originally purified from the bacterium Streptomyces kanamyceticus. Kanamycin acts by binding to the 30S subunit of the bacterial ribosome and inhibiting protein synthesis in susceptible bacteria. Gibco® Kanamycin is effective against a wide variety of gram-negative and some gram-positive bacteria, and ...

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  • Kanamycin Monosulfate 50 mg/mL Solution | GoldBio

    Kanamycin is an aminoglycoside antibiotic isolated from Streptomyces kanamyceticus. It is effective against gram-positive and gram-negative bacteria as well as Mycoplasma species. Kanamycin is commonly used to select for bacteria that have been transformed with a kanamycin-resistant gene and plant tissues that have the NPT II ( APH3) gene ...

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  • Kanamycin Monosulfate 25389-94-0 | TCI AMERICA

    Kanamycin, an aminoglycoside antibiotic, was first isolated in 1957 from the bacterium Streptomyces kanamyceticus by Umezawa. Kanamycin has broad activity against Gram-negative bacteria and Gram-positive bacteria, including Mycobacterium tuberculosis. Kanamycin interacts with the 30S subunit of prokaryotic ribosomes, and then it inhibits bacterial growth by inhibiting protein synthesis.

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  • Final text for addition to The International Pharmacopoeia

    Kanamycin monosulfate is produced by methods of manufacture designed to eliminate or minimize substances lowering blood pressure. The method of manufacture is validated to demonstrate that the product, if tested, would comply with the test as described under 3.7 Undue toxicity, using 0.5 ml of a solution containing 2 mg per ml.

    Get Price
  • Final text for addition to The International Pharmacopoeia

    Kanamycin acid sulfate is produced by methods of manufacture designed to eliminate or minimize substances lowering blood pressure. The method of manufacture is validated to demonstrate that the product, if tested, would comply with the test as described under 3.7 Undue toxicity, using 0.5 ml of a solution containing 2 mg per ml. Identity tests

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  • Kanamycin Sulfate - Thermo Fisher Scientific

    Kanamycin Sulfate is a water-soluble antibiotic originally purified from the bacterium Streptomyces kanamyceticus. Kanamycin acts by binding to the 30S subunit of the bacterial ribosome and inhibiting protein synthesis in susceptible bacteria. Gibco Kanamycin is effective against a wide variety of g

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  • A fluorometric aptamer method for kanamycin by

    2005-6-13 · Identification Summary. Kanamycin is an aminoglycoside antibiotic agent used in the treatment of various infections caused by susceptible bacteria.. Generic Name Kanamycin DrugBank Accession Number DB01172 Background. Kanamycin (also known as kanamycin A) is an aminoglycoside bacteriocidal antibiotic, available in oral, intravenous, and intramuscular forms, and used to treat a …

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  • Kanamycin: Uses, Interactions, Mechanism of Action ...

    Kanamycin, an aminoglycoside antibiotic, was first isolated in 1957 from the bacterium Streptomyces kanamyceticus by Umezawa. Kanamycin has broad activity against Gram-negative bacteria and Gram-positive bacteria, including Mycobacterium tuberculosis.Kanamycin interacts with the 30S subunit of prokaryotic ribosomes, and then it inhibits bacterial growth by inhibiting protein synthesis.

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  • Kanamycin Monosulfate 25389-94-0 | TCI AMERICA

    2017-9-20 · KAT = Kanamycin 6'-Acetyltransferase CoA = Coenzyme A DTNB = 5,5'-Dithio-bis-(2-Nitrobenzoic Acid) TNB = 5-Thio-2-Nitrobenzoic Acid CONDITIONS: T = 37°C, pH = 5.7, A412nm, Light path = 1 cm METHOD: Continuous Spectrophotometric Rate Determination REAGENTS: A. 100 mM MES Buffer, pH 5.7 at 37°C (Prepare 100 ml in deionized water using MES, Free ...

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  • Enzymatic Assay of KANAMYCIN 6'-ACETYLTRANSFERASE

    2021-4-27 · Results By using this novel method, kanamycin, sulfate and related substances were well separated. The precision, the repeatability and the recoveries of this method were all acceptable. There was a good linear relationship between the kanamycin concentration and the peak area (R2>0.99).

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  • Gas-liquid chromatographic determination of amino ...

    2020-12-1 · Development of liquid chromatographic method for the analysis of kanamycin residues in varicella vaccine using phenylisocyanate as a derivatization reagent. Journal of Chromatography B: Biomedical Sciences and Applications 2001 , 752 (1) , 173-177.

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  • The crystal structure of kanamycin - ScienceDirect

    The present study on the crystal structure of kanamycin has not on1y confirmed the structure already known, but also justified the configurational chemical studies about the positions C and C of the deoxystreptamine moiety by a copper complex method The structure in Fig. 3 here presented conforms with the chemical observations, that is ...

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  • Kanamycin Sulfate | CAS:25389-94-0 | Broad spectrum ...

    A number of commercial samples were analyzed using this method. Besides the previously reported impurities, such as kanamycins B and C, two other impurities were separated, one of which is called kanamycin D. In total, eight components were separated. [Research progress of acute kanamycin sulfate-induced deafness in guinea pig].[Pubmed:22870728]

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  • Kanamycin: Uses, Interactions, Mechanism of Action ...

    2021-4-27 · Results By using this novel method, kanamycin, sulfate and related substances were well separated. The precision, the repeatability and the recoveries of this method were all acceptable. There was a good linear relationship between the kanamycin concentration and the peak area (R2>0.99).

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  • Modulation of kanamycin B and kanamycin A

    2005-6-13 · Identification Summary. Kanamycin is an aminoglycoside antibiotic agent used in the treatment of various infections caused by susceptible bacteria.. Generic Name Kanamycin DrugBank Accession Number DB01172 Background. Kanamycin (also known as kanamycin A) is an aminoglycoside bacteriocidal antibiotic, available in oral, intravenous, and intramuscular forms, and used to treat a …

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  • Resonance light scattering method for detecting

    2017-7-28 · Both kanamycin A and kanamycin B, antibiotic components produced by Streptomyces kanamyceticus, have medical value. Two different pathways for kanamycin biosynthesis have been reported by two research groups. In this study, to obtain an optimal kanamycin A-producing strain and a kanamycin B-high-yield strain, we first examined the native kanamycin biosynthetic pathway in vivo.

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  • Dane K. Fisher and Mark J. Guiltinan* Department of ...

    2017-2-6 · In this paper, a resonance light scattering (RLS) method was developed for detecting kanamycin with high sensitivity and selectivity. Here, aptamer specific to kanamycin was utilized for recognizing and competitively binding with kanamycin, and gold nanoparticles (GNPs) were used as probes. After adding kanamycin into solutions containing aptamer and GNPs, the GNPs would …

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  • Gas-liquid chromatographic determination of amino ...

    2012-9-17 · Kanamycin monosulfate (Sigma K 1377) was prepared as a 50 mg/ml stock solution in distilled water and filter sterilized. Stock solution was prepared fresh for each experiment or stored at -20C for no longer than 2 weeks. Carbenicillin (Sigma C 3416) was prepared as a 50 mg/ml stock solution in distilled water and filter sterilized.

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  • Reference standards for microbiologicalassayof antibiotics

    2002-5-1 · Development of liquid chromatographic method for the analysis of kanamycin residues in varicella vaccine using phenylisocyanate as a derivatization reagent. Journal of Chromatography B: Biomedical Sciences and Applications 2001, 752 (1) , 173-177. DOI: 10.1016/S0378-4347(00)00506-5.

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  • Synthesis of Ring II/III Fragment of Kanamycin: A New ...

    2020-2-19 · Method B:Turbidimetricassay •Referenceand test solutions are distributedintotest tubes •Inoculatedmedium isadded •Tubes are incubatedin a water-bath for ... Kanamycin Monosulfate Neomycin Sulfate for microbiological assay NetilmicinSulfate Nystatin PolymyxinB Sulfate for microbiological assay RifamycinSodium Spiramycin

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  • Agrobacterium mediated transformation of Vigna ...

    A novel protocol has been established to prepare the kanamycin ring II/III fragment, which has been validated as a minimum structural motif for the development of new aminoglycosides on the basis of its bactericidal activity even against resistant strains. Furthermore, its ability to act as a AAC-(6′) and APH-(3′) binder, and as a poor substrate for the ravenous ANT-(4′), makes it an ...

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  • Dane K. Fisher and Mark J. Guiltinan* Department of ...

    2012-9-17 · Kanamycin monosulfate (Sigma K 1377) was prepared as a 50 mg/ml stock solution in distilled water and filter sterilized. Stock solution was prepared fresh for each experiment or stored at -20C for no longer than 2 weeks. Carbenicillin (Sigma C 3416) was prepared as a 50 mg/ml stock solution in distilled water and filter sterilized.

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  • Treatment with Sub-inhibitory Kanamycin Induces

    2015-1-28 · kanamycin (30 µg/ml) agar plates. A doubly-sensitive colony was selected, renamed STVV11W-1, and used in all subsequent assays. Antibiotic stock solutions. Stock solutions of kanamycin monosulfate (Sigma #K-4000) and streptomycin sulfate (Sigma #S-6501) were prepared by dissolving each antibiotic in distilled water to

    Get Price
  • Kanamycin sulfate ≥94.0% (by total nitrogen basis) |

    Avantor ®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries.Our portfolio is used in virtually every stage of the most important research, development and production activities in the industries we serve.

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  • Agrobacterium mediated transformation of Vigna ...

    2015-2-18 · potassium-clavunalate, 200; and kanamycin monosulfate, 50. After two weeks, 20 explant~ putatively transformed with EHA 10 I were placed or selective medium with increasing concentrations o1 kanamycin (up to 200 mg/L) and L-phosphinotricir (I 0 mg/L; Hoechst, Frankfurt), at …

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  • THE J B C ...

    2021-3-3 · sequencingwithsodiumbisulfite.Kanamycin,hygromy-cin,andcefotaxime(alsocalledClaforan)arecommonly ... with one of kanamycin monosulfate (Sigma), hygromycin B (Calbio- ... using the method of Bestor et al. (8). DNA from plantlets treated with cefotaxime (500 mg/liter) and control plantlets ...

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  • Reference standards for microbiologicalassayof antibiotics

    2020-2-19 · Method B:Turbidimetricassay •Referenceand test solutions are distributedintotest tubes •Inoculatedmedium isadded •Tubes are incubatedin a water-bath for ... Kanamycin Monosulfate Neomycin Sulfate for microbiological assay NetilmicinSulfate Nystatin PolymyxinB Sulfate for microbiological assay RifamycinSodium Spiramycin

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  • PROPOSAL TO DISCONTINUE THE TEST FOR UNDUE

    2021-3-3 · 120 Kanamycin monosulfate is produced by methods of manufacture designed to eliminate or 121 minimize substances lowering blood pressure. The method of manufacture is validated to 122 demonstrate that the product, if tested, would comply with the test as described under 3.7 123 Undue toxicity, using 0.5 mL of a solution containing 2 mg per mL. 124

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  • Synthesis of Ring II/III Fragment of Kanamycin: A New ...

    A novel protocol has been established to prepare the kanamycin ring II/III fragment, which has been validated as a minimum structural motif for the development of new aminoglycosides on the basis of its bactericidal activity even against resistant strains. Furthermore, its ability to act as a AAC-(6′) and APH-(3′) binder, and as a poor substrate for the ravenous ANT-(4′), makes it an ...

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  • Process for the preparation of 1-[L-(-)-γ-amino-α ...

    1-[L-(-)-γ-amino-α-hydroxybutyryl]-kanamycin A is a compound useful as an antibacterial agent. A new process for its preparation has been developed which comprises using aldehyde blocking agents on the amine functions prior to acylation with L-(-)-γ-amino-α-hydroxybutyric acid.

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  • Kanamycin: Indications, Side Effects, Warnings -

    Drugs.com provides accurate and independent information on more than 24,000 prescription drugs, over-the-counter medicines and natural products. This material is provided for educational purposes only and is not intended for medical advice, diagnosis or treatment. Data sources include IBM Watson Micromedex (updated 1 July 2021), Cerner Multum™ (updated 1 July 2021), ASHP (updated 30 June ...

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  • Treatment with Sub-inhibitory Kanamycin Induces

    2015-1-28 · kanamycin (30 µg/ml) agar plates. A doubly-sensitive colony was selected, renamed STVV11W-1, and used in all subsequent assays. Antibiotic stock solutions. Stock solutions of kanamycin monosulfate (Sigma #K-4000) and streptomycin sulfate (Sigma #S-6501) were prepared by dissolving each antibiotic in distilled water to

    Get Price
  • VALVEKENS'S PROTOCOL: A GUIDE TO HIGH EFFICIENCY ...

    2019-7-11 · sterile water, and filter sterilise using an Acrodisk. You can filter pouring the solution in the flask with the medium, or in a tube. Never leave the augmentin stock solution on ice or freeze it. It will go off (becomes brown). KANAMYCIN [email protected] mgml-1 Prepare a few ml. Sterilise through an Acrodisk and make aliquots of 50 ul in the ...

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  • The down regulation of E. coli OmpF in response to sub ...

    2015-1-28 · for Ampicillin (Sigma #A9518) and Kanamycin monosulfate (Sigma #K4000) solutions. All antibiotic stock solutions were subsequently filter-sterilized using a 0.22 µm Millipore filter (#HAWP01300) before storage at 4°C. Kanamycin resistance cassette removal using pCP20. For each of the JW5249 (ΔmarA) and JW0912

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  • Agrobacterium mediated transformation of Vigna ...

    2015-2-18 · potassium-clavunalate, 200; and kanamycin monosulfate, 50. After two weeks, 20 explant~ putatively transformed with EHA 10 I were placed or selective medium with increasing concentrations o1 kanamycin (up to 200 mg/L) and L-phosphinotricir (I 0 mg/L; Hoechst, Frankfurt), at …

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  • Trichostatin A Enhances Gap Junctional Intercellular ...

    kanamycin monosulfate, 10 lg/ml sodium ampicillin, and 50 lg/ml strepto-mycin sulfate), and 10% vol/vol fetal bovine serum. After 4 h, the medium was removed and renewed with fresh medium supplemented with 25 lg/ml hydrocortisone hemisuccinate and 0.25 lg/ml amphotericin B. After 24 h, the medium was renewed daily with serum-free medium.

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  • Mycobacterium smegmatis D-Alanine Racemase Mutants

    2021-3-19 · method using cetyl trimethyl ammonium bromide (1). Plasmid DNA was isolated by an alkaline lysis method as previously described (36), using a large-scale isolation kit (Promega, Madison, Wis.) as recommended by the manufacturer. DNA fragments used for plasmid construction in E. coli and for recombination

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  • Team:Shanghai HS/Notebook - 2019.igem.org

    2019-12-19 · B.Inject 500microliter of 2000x Kanamycin Monosulfate into the large flask with 1L of capacity C.Place the solution in the centrifuge at 220 rpm 37 degrees Celsius till OD reaches the range of 0.6~0.8 D. Lower the temperature to 18 degrees Celsius, add 350microliter of 1 mole IPTG Place the solution in the centrifuge at 190 rpm 18 degrees ...

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  • Resazurin Microtiter Assay Plate method for

    Egyptian Journal of Chest Disease and Tuberculosis (2013-04-01) . Resazurin Microtiter Assay Plate method for detection of susceptibility of multidrug resistant Mycobacterium tuberculosis to second-line anti-tuberculous drugs

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  • PPT – Heterocyclic antibiotics: aminoglycosides ...

    Kanamycin monosulfate at the heating with a ninhydrin solution observed violet color. Melting temperature of Kanamycin picrate. They give reaction of sulfate. Bials reaction (pentose opening). For the identification of Kanamycin monosulfate and neomycin sulfate using a color reaction with a alcohol solution of an orcyn (methylresorcinol)

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  • Kanamycin. Medical search. Frequent questions

    2021-7-14 · kanamycin. Frequent questions. Medical Information Search. Amikacin 11. Another mechanism(s) may account for high-level amikacin-kanamycin resistance in two mutants and lower levels of resistance in four clinical isolates, all lacking the A1400G mutation.(We examined resistance in Mycobacterium tuberculosis (MTB) to the deoxystreptamine aminoglycosides amikacin (AK) and kanamycin …

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  • SUPPORTING INFORMATION A General Method for

    2021-3-12 · A General Method for Fluorescent Labeling of the N-termini of Lanthipeptides and Its ... thiogalactopyranoside (IPTG) (Gold Biotechnology), kanamycin monosulfate (IBI Scientific), chloramphenicol (IBI Scientific), spectinomycin dihydrochloride pentahydrate (Sigma Aldrich), ... Cy5 analogues were excited using a 639 nm laser and emission was ...

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  • Treatment with Sub-inhibitory Kanamycin Induces

    2015-1-28 · kanamycin (30 µg/ml) agar plates. A doubly-sensitive colony was selected, renamed STVV11W-1, and used in all subsequent assays. Antibiotic stock solutions. Stock solutions of kanamycin monosulfate (Sigma #K-4000) and streptomycin sulfate (Sigma #S-6501) were prepared by dissolving each antibiotic in distilled water to

    Get Price
  • Kanamycin sulfate ≥94.0% (by total nitrogen basis) |

    Avantor ®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries.Our portfolio is used in virtually every stage of the most important research, development and production activities in the industries we serve.

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  • Aggregation of Kanamycin A: dimer formation with ...

    2011-3-2 · Kanamycin A dimer. The global optimization of the KA dimer with different physiological cations was carried out using OGOLEM and AMBER with the GAFF force field. Multiple successive runs were carried out, employing a number of global optimization iterations large enough to yield the same result in most of them.

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  • THE J B C ...

    2021-3-3 · sequencingwithsodiumbisulfite.Kanamycin,hygromy-cin,andcefotaxime(alsocalledClaforan)arecommonly ... with one of kanamycin monosulfate (Sigma), hygromycin B (Calbio- ... using the method of Bestor et al. (8). DNA from plantlets treated with cefotaxime (500 mg/liter) and control plantlets ...

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  • Synthesis of Ring II/III Fragment of Kanamycin: A New ...

    A novel protocol has been established to prepare the kanamycin ring II/III fragment, which has been validated as a minimum structural motif for the development of new aminoglycosides on the basis of its bactericidal activity even against resistant strains. Furthermore, its ability to act as a AAC-(6′) and APH-(3′) binder, and as a poor substrate for the ravenous ANT-(4′), makes it an ...

    Get Price
  • The down regulation of E. coli OmpF in response to sub ...

    2015-1-28 · for Ampicillin (Sigma #A9518) and Kanamycin monosulfate (Sigma #K4000) solutions. All antibiotic stock solutions were subsequently filter-sterilized using a 0.22 µm Millipore filter (#HAWP01300) before storage at 4°C. Kanamycin resistance cassette removal using pCP20. For each of the JW5249 (ΔmarA) and JW0912

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  • Measurement of the Promoter Activity in Escherichia

    2020-1-20 · 50 mg/ml kanamycin. Dissolve 0.5 g of kanamycin monosulfate (Nacalai Tesque) in 10 ml of distilled water. Sterilize the solution by filtration with 0.22 μm filter (Advantec) Store the sterilized solution at 4 °C. LB broth. Dissolve 10 g of Bacto TM tryptone, 5 g of Bacto TM yeast extract, and 10 g of NaCl in 800 ml of distilled water

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  • VALVEKENS'S PROTOCOL: A GUIDE TO HIGH EFFICIENCY ...

    2019-7-11 · sterile water, and filter sterilise using an Acrodisk. You can filter pouring the solution in the flask with the medium, or in a tube. Never leave the augmentin stock solution on ice or freeze it. It will go off (becomes brown). KANAMYCIN [email protected] mgml-1 Prepare a few ml. Sterilise through an Acrodisk and make aliquots of 50 ul in the ...

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  • (PDF) Inhibition of connexin hemichannels alleviates

    Viable ( ≥85%) hepatocytes were plated at a density of 0.56 × 105 cells per cm2 in William’s medium E (Invitrogen, USA) supplemented with 7 ng/mL gluca- gon, 292 mg/mL L-glutamine, antibiotics (7.33 IU of sodium benzyl penicillin, 50 μg/mL kanamycin monosulfate, 10 μg/mL sodium ampicillin, and 50 μg/ml streptomycin sulfate), and 10% ...

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  • Production of Male Sterile Interspecific Somatic Hybrids ...

    Using this method, sets of restriction fragments may be ... (1 mg 1-1) BA, 3 mg 1-' bialaphos and 50 mg 1-1 kanamycin monosulfate. Shoot induction was achieved after 30 d of cultivation on the same medium. The plants were rooted in MS basic medium supplied with Morel vitamins

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  • Scientific Protocols - Genetic transformation of ...

    2008-3-20 · 50 mg ml-1 kanamycin: Dissolve 2 g kanamycin monosulfate in 30 ml of distilled water, bring to a final volume of 40 ml. Filter-sterilize (0.2 µm) and store at -20 °C. 100 mg ml-1 hygromycin B: Add 9 ml of distilled water to 1 ml hygromycin B (1 g ml-1). Filter-sterilize (0.2 µm) and store at -20 °C.

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  • Reflection paper providing an overview of the current ...

    Griseofulvin, Kanamycin acids sulphate, Kanamycin monosulfate, Nystatin, Rifamycin sodium. This test is included inthe Production section of the Ph.Eur. monographs of some active substances, therefore its performance is not required as a routine, since the Ph.Eur. establishes that “the method of manufacture is validated to demonstrate that the

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  • AcrS Is a Potential Repressor of acrA Expression in coli ...

    2016-12-22 · Resistance to kanamycin in E. coli JW0452-3 (ΔacrA), E. coli JW3232- 1 (Δ acrS) and E. coli BW25113 (wild-type) was compared using minimum inhibitory concentration (MIC) assays. MIC assay results showed that the Δ acrS strain had a higher resistance to kanamycin compared to both Δ acrA and

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  • Enhanced Accumulation of Metals in Bottle Gourd Plants ...

    2015-11-6 · Transgenic Plant Journal 2 (2), 165-169 ©2008 Global Science Books OD 600 = 1.0 was obtained. Bottle gourd (Lagenaria siceraria Standl.) transformation was performed via Agrobacterium-mediated transformation method using the cotyledon explants of G5 inbred line as previously repor-ted (Han et al. 2005) except that 100 mg·L–1 kanamycin monosul- fate was used as a selective agent for ...

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  • Targeted Single-Walled Carbon Nanotubes for

    2021-1-7 · ized using atomic force microscopy (AFM) to show that ... Tryptone, yeast extract, and kanamycin monosulfate were obtained from Alfa Aesar (Ward Hill, MA). Sodium hydroxide, potassium chloride, and sodium chloride were from VWR Inc (Radnor, PA). ... . e CoMoCAT method is known to yield SWCNT of a small number of (,m) chiralities n with high ...

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  • Cytokinin‐induced protein synthesis suppresses

    2020-3-4 · Kanamycin resistance of the Arabidopsis arr1-1 mutant is reversed by overexpression of wild-type and phosphomimic ARR1. (a) Rosettes of representative 3-wk-old Col-0 (WT) and arr1-1 plants as well as plants of five independent 35S::ARR1 transgenic lines generated in the arr1-1 background (ARR1 OE) grown on control media and media containing 35 mg l −1 kanamycin (Km).

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  • High-Speed Force Spectroscopy for Single Protein

    2021-7-14 · This method provides information about the mechanisms of folding and unfolding under an applied force, allowing the characterization of the (un)folding energy landscape along the axis of applied force. FS using AFM is a well-established technique, however, the time resolution is limited to a few hundred of µs [15].

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  • Recombinant Insulin Production In E. Coli |

    1.06 Oxygen Flask Combustion Method 1.07 Heavy Metals Limit Test 1.08 Nitrogen Determination (Semimicro-Kjeldahl Method) 1.09 Qualitative Tests 1.10 Iron Limit Test 1.11 Arsenic Limit Test 1.12 Methanol Test 1.13 Fats and Fatty Oils Test 1.14 Sulfate

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  • kanamycin: Topics by Science.gov

    Thirty-nine patients of the test group were treated with metronidazole and kanamycin monosulfate. Kanamycin monosulfate was used 3 days before the operation in a dose of 0.5 g orally 4 times a day whereas metronidazole in a dose of 0.5 g 3 times a day. The last doses of the drugs were administered 4-5 hours before the operation.

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  • SAFETY DATA SHEET

    Kanamycin Sulfatea Revision Date 23-January-2018 5. Fire-fighting measures Suitable Extinguishing MediaUse water spray, alcohol-resistant foam, dry chemical or carbon dioxide. Unsuitable Extinguishing MediaNo information available Flash Point Not applicable Method - …

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  • Characterization of a Ferrous Iron-Responsive Two ...

    2012-8-13 · Using this method, we determined that FirRS was unresponsive to epinephrine, norepinephrine, and serotonin at concentrations up to 200 μM. We were also unable to elicit a response using spent media or fractions obtained by the AI-3 purification method described by Clarke et al. .

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  • Resazurin Microtiter Assay Plate method for detection

    Results of REMA were available after 8days of incubation. The agreement between the two tests for para-aminosalicylic acid, ethionamide, ofloxacin, kanamycin monosulfate and cycloserine was found to be 97.05%, 94.11%, 97.05%, 97.05% and 100%, respectively.

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  • U.S. Pharmacopeia

    2017-6-29 · guanethidine monosulfate tablets guanfacine tablets guanidine hydrochloride guanidine hydrochloride tablets ... <1098> validation of assays using commercial kits lubiprostone capsules ... kanamycin sulfate capsules kanamycin injection sterile kanamycin sulfate …

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  • Measurement of the Promoter Activity in Escherichia coli ...

    2021-4-30 · Clone a target promoter as the purified PCR product into the liner pLUX using In-Fusion HD. Cloning Plus (Takara Bio). Note: We recommend in vivo E. coli cloning (iVEC) as an alternative DNA cloning method (Nozaki et al., 2019), which is provided from National BioResource Project (NBRP) of Japan. 7. Add a part (~10 . μ. l) of the In-

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  • Discovery of Reactive Microbiota-Derived Metabolites

    Discovery of Reactive Microbiota-Derived Metabolites that Inhibit Host Proteases. Chun-Jun Guo. Chun-Jun Guo. Affiliations. Department of Bioengineering and Therapeutic Sciences and California Institute for Quantitative Biosciences, University of California, San Francisco, San Francisco, CA 94158, USA. Search for articles by this author.

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  • CRS catalogue

    2017-7-13 · CRS catalogue. Please enter a search term and select a search method using the drop menus below. If you select 'contains', all entries containing your search term will be returned. For example, if you enter 'toco', both 'tocopherol' and 'ketoconazole' will be returned.

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