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jiebaikeli for influenza using method

  • Influenza surveillance: determining the epidemic

    Influenza surveillance: determining the epidemic threshold for influenza by using the Moving Epidemic Method (MEM), Montenegro, 2010/11 to 2017/18 influenza seasons. Our study marks the first attempt to determine the pre/post-epidemic threshold values for the epidemic period in Montenegro.

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  • Using an innovative method to develop the threshold

    2018-8-31 · The potential misclassification proportion of influenza epidemic waves was 16% for method I but was zero for method II and 6% for MLRM. When 1/2 and 1/4 of the median was used as the threshold, the potential misclassification proportion was 15% and 18% for method I, 0 to 1% for method II, and 4% and 14% for MLRM, respectively.

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  • Cumulative Query Method for Influenza Surveillance

    2014-12-16 · Studies using Google Trends for influenza surveillance show that it can be used as a complementary source of data but that its performance is insufficient for use as a model for prediction [33,34]. It is difficult to find queries that show high correlations for consecutive years because Internet searching behavior may change over time [33,34]. To reduce the effects from changes in search …

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  • A novel screening method for influenza patients using

    2010-4-1 · In summary, the discriminant function of this proposed system is effective in distinguishing influenza patients regardless of their temperatures, which enables higher PPV rate of 93% compared to the conventional method using only thermography. There are some limitations in detecting infected individuals before symptoms appear.

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  • A rapid method for immunotitration of influenza

    2003-6-9 · MDCK is historically the cell line of choice for plaque assay for influenza virus, as these cells support productive infection and plaque formation of most strains of A and B influenza viruses (Gaush and Smith, 1968, Tobita, 1975, Tobita et al., 1975). Although sensitive, plaque assays are time consuming and labour intensive, requiring up to several days depending on the virus type, subtype and source.

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  • Applying the moving epidemic method to determine

    2020-5-10 · The MEM is a tool developed to better understand annual influenza epidemics and assess the epidemic status and intensity on a weekly basis. 8, 9 The method was progressively improved and implemented in European documents by the ECDC and WHO. 15 Later on, it became widely used in many countries outside Europe such as USA, Australia, New Zealand, and Canada. 6, 10, 16, 17 Other countries have opted for the method proposed by WHO and based on the peak mean values of influenza …

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  • Immunofluorescence methods for detection of

    Indirect immunofluorescence technique was used for detection of specific antibodies against influenza and mumps viruses in the IgM and IgG fractions of human gamma globulins. Infected cells shed into the allantoic fluid from the allantoic membrane of inoculated hen's eggs served as a source of cell-bound influenza antigens.

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  • Influenza surveillance in Europe: comparing intensity ...

    Design and setting: Weekly ILI and ARI incidence rates and proportion of primary care consultations were modeled in 28 countries for the 1996/1997-2013/2014 seasons using the moving epidemic method (MEM). We calculated the epidemic threshold and three intensity thresholds, which delimit five intensity levels: baseline, low, medium, high, and very high.

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  • Development of a Specific and Rapid Diagnostic

    2013-12-1 · Although rRT-PCR is a standard method and is highly sensitive and specific for the detection of influenza viruses 5, 6, 7, it requires specialized equipment, trained technicians, and takes more than 5–24 hours to generate results from clinical specimens 1, 7, 8; in addition, rRT-PCR is available only in limited diagnostic laboratories 9, 10.

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  • Quantification and characterization of virus-like ...

    2017-3-3 · The LLOD and LLOQ for the SEC-UV method were calculated according to the recommendations made by ICH Q2 using the approach based on the standard deviation of the response signal and the slope of the calibration curve ; the limits are expressed as LLOD=3σ/S and LLOQ=10σ/S, with σ as the residual standard deviation and S as the slope of the calibration curve. The ULOQs were defined from the highest concentration reproducibly analyzed (RSD <15%) within the linear range of the method.

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  • Overview of Influenza Testing Methods | CDC

    Overview. Influenza virus testing is not required to make a clinical diagnosis of influenza in outpatients with suspected influenza, particularly during increased influenza activity when seasonal influenza A and B viruses are circulating in the local community. However, influenza virus testing can inform clinical management when the results may ...

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  • Using an innovative method to develop the threshold

    2018-8-31 · Introduction. Seasonal influenza is a major contributor to global burden of diseases and injuries. Each year, about 5%-10% of adults and 20%-30% of children suffer from seasonal influenza worldwide, leading to three to five million severe cases and 0.25 to 0.50 million deaths [].To effectively allocate resources necessary for controlling seasonal epidemics of influenza, public health ...

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  • A rapid method for immunotitration of influenza

    2003-6-9 · By using a panel of viruses of different type, subtype and origin, it is demonstrated that there is a mathematical correlation between titres measured by immunotitration and by classical plaque assay on MDCK cells. Moreover, the availability of NP antibodies specific for type A or type B influenza virus ensures the specificity of the assay.

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  • Computational Method for Classification of Avian

    Furthermore, a machine learning method was also devised to predict global reservoirs for low pathogenic avian influenza virus using big data (Gulyaeva et al., 2020) but there was no specific strategy to classify only Avian Influenza A virus subtypes, so there was a need to develop a method.

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  • A novel screening method for influenza patients using

    2010-4-1 · Table 1. Z values of the normal group and the influenza group as calculated from data, which is shown in part in this table (heart rate, respiratory rate, and average facial temperature) using linear discriminant analysis. All Z values are positive in the normal group and are negative in the influenza group. The linear discriminant function Z(X 1,X 2,X 3) calculated from the non-contact ...

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  • An easy, rapid, and sensitive method for detection of

    2018-7-12 · By using the method in combination with virus-concentrated membranes in centrifugal filter units and a sialidase imaging probe, 2-(benzothiazol-2-yl)-4-bromophenyl-N-acetylneuraminic acid (BTP3-Neu5Ac), sialidase activity of influenza neuraminidase was visualized on membranes by the green fluorescence of produced hydrophobic BTP3 under UV ...

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  • A simplified Sanger sequencing method for full

    2015-7-1 · 1. Background. Influenza viruses belong to the family of Orthomyxoviridae RNA viruses. Influenza A viruses (IAV) contain eight segmented negative strand RNAs of approximately 13.5 kb which encode at least 12 proteins, including polymerase basic protein 2 (PB2) and 1 (PB1), PB1-F2, polymerase acidic protein (PA) and PA-X, hemagglutinin (HA), nucleoprotein (NP), neuraminidase (NA), matrix ...

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  • Using a fast clustering method for viral segment lineage ...

    2017-8-18 · Using a fast clustering method for viral segment lineage determination, applied to the H9 influenza hemagglutinin. Andrew R. Dalby Department of Biomedical Sciences, University of Westminster, 115 New Cavendish Street, Westminster, W1W 6UW, UK …

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  • Evaluation of A Single-reaction Method for Whole

    2016-1-1 · Objective. To evaluate a single-reaction genome amplification method, the multisegment reverse transcription-PCR (M-RTPCR), for its sensitivity to full genome sequencing of influenza A virus, and the ability to differentiate mix-subtype virus, using the next generation sequencing (NGS) platform.

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  • US20210060153A1 - Method for preparing influenza

    The present invention relates to a method of preparing an influenza working virus seed stock, a method of increasing infectivity of an influenza working virus seed stock, a method of preparing an influenza vaccine using the seed stock, an influenza vaccine prepared by the method, and an influenza working virus seed stock having increased infectivity.

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  • Using an innovative method to develop the threshold

    2018-8-31 · Introduction. Seasonal influenza is a major contributor to global burden of diseases and injuries. Each year, about 5%-10% of adults and 20%-30% of children suffer from seasonal influenza worldwide, leading to three to five million severe cases and 0.25 to 0.50 million deaths [].To effectively allocate resources necessary for controlling seasonal epidemics of influenza, public health ...

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  • A Spatial-Temporal Method to Detect Global Influenza ...

    2017-4-4 · Abstract: The 2009 influenza pandemic teaches us how fast the influenza virus could spread globally within a short period of time. To address the challenge of timely global influenza surveillance, this paper presents a spatial-temporal method that incorporates heterogeneous data collected from the Internet to detect influenza epidemics in real time.

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  • A Spatial-Temporal Method to Detect Global Influenza ...

    The 2009 influenza pandemic teaches us how fast the influenza virus could spread globally within a short period of time. To address the challenge of timely global influenza surveillance, this paper presents a spatial-temporal method that incorporates heterogeneous data collected from the Internet to detect influenza epidemics in real time.

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  • A method for the formulation of influenza virus

    The relationship of new 'field' isolates of influenza virus to previously isolated strains might not be evident by inspection of haemagglutination-inhibition data. Numerical taxonomic methods have been applied to experimental results in order to clarify these relationships so that antigenic trends may be more easily seen. The diagrammatic representations so derived suggest that numerical...

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  • Rapid and Sensitive Method Using Multiplex Real

    2019-8-13 · influenza surveillance systems: sentinel primary care influenza-like illness versus severe hospitalized laboratory-confirmed influenza using the moving epidemic method Núria Torner1,2,3*, Luca Basile1, Ana Martínez1,2, Cristina Rius2,4, Pere Godoy1,2, Mireia Jan ...

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  • Assessment of two complementary influenza surveillance ...

    Method of preventing or treating influenza A viral infection using cloned DI influenza A viral particles . United States Patent 9089516 . Abstract: Cloned, i.e. defined, defective interfering (DI) influenza A virus is produced in embryonated hens eggs using a method which generates large quantities of DI virus material. Co-administration of ...

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  • Method of preventing or treating influenza A viral ...

    2013-10-29 · Influenza is a rapidly changing virus that appears seasonally in the human population. Every year a new strain of the influenza virus appears with the potential to cause a serious global pandemic. Knowledge of the structure and density of the surface proteins is of …

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  • Computational Methods for Electron Tomography of

    2017-1-1 · Using a capture–recapture method, we estimated influenza hospitalization rates of 0.87 (95% CI 0.06–1.68) and 0.80 (95% CI 0.62–0.98) per 1,000 persons among children and adults, respectively. Disclosures N. B. Halasa, sanofi pasteur: Research Contractor, Research support. Astra Zeneca: Research Contractor, Grant recipient. W.

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  • Estimated Hospitalization Rates for Seasonal Influenza

    2012-6-19 · As influenza cases disperse nationwide and an epidemic reaches its peak, WSD value changed to be a progressively increasing. Conclusions. The spatial distribution of nationwide influenza outbreak was measured by using a novel WSD method. We showed that spreading rate varied by type and subtypes of influenza virus using WSD as a spatial indicator.

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  • Influenza Virus Testing Methods | CDC

    Table 1: Influenza Virus Testing Methods. Method 1. Types Detected. Acceptable Specimens 2. Test Time. CLIA Waived 3. Rapid Influenza Diagnostic Tests4 (antigen detection) A and B. NP 5 swab, aspirate or wash, nasal swab, aspirate or wash, throat swab.

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  • Overview of Influenza Testing Methods | CDC

    Overview. Influenza virus testing is not required to make a clinical diagnosis of influenza in outpatients with suspected influenza, particularly during increased influenza activity when seasonal influenza A and B viruses are circulating in the local community. However, influenza virus testing can inform clinical management when the results may ...

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  • Methods for detecting seasonal influenza epidemics

    2019-9-5 · School absenteeism data have been collected daily by the public health unit in Wellington-Dufferin-Guelph, Ontario since 2008. To date, a threshold-based approach has been implemented to raise alerts for community-wide and within-school illness outbreaks. We investigate several statistical modelling approaches to using school absenteeism for influenza surveillance at the regional level, and ...

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  • Using the MEM method for influenza seasonality

    2017-7-26 · Using the MEM method for influenza seasonality determination Dr. Tomás Vega Alonso Dirección General de Salud Pública. Consejería de Sanidad. [email protected] 1 . 2 The problem Influenza: great variation in timing and intensity. Other IRV: multiple causes, overlapping of waves.

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  • Computational Method for Classification of Avian

    2021-1-28 · Furthermore, a machine learning method was also devised to predict global reservoirs for low pathogenic avian influenza virus using big data (Gulyaeva et al., 2020) but there was no specific strategy to classify only Avian Influenza A virus subtypes, so there was a need to develop a method.

    Get Price
  • A novel screening method for influenza patients using

    Objectives - In places of mass gathering, rapid infection screening prior to definite diagnosis is vital during the epidemic season of a novel influenza. In order to assess the possibility of clinical application of a newly developed non-contact infection screening system, we conducted screening for influenza patients. Materials and methods - The system is operated by a screening program via a...

    Get Price
  • An optimized molecular method for detection of

    2020-12-2 · An optimized molecular method for detection of influenza A virus using improved generic primers and concentration of the viral genomic RNA and nucleoprotein complex Ji-Woon Kim,* Chung-Young Lee,* Thanh Trung Nguyen, Il-Hwan Kim, Hyuk-Joon Kwon,1 Jae-Hong Kim Abstract.

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  • ELISA method for detection of influenza A infection in swine

    2021-7-10 · ELISA method for diagnosis of influenza in swine 511 capture ELISA for the direct detection of H1N1 influ-enza virus in swine. This ELISA was compared with the standard egg isolation method in evaluating the duration of virus shedding in swine experimentally infected with a field strain of H1N1. Materials and methods Viruses.

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  • Using a fast clustering method for viral segment lineage ...

    2017-8-18 · Using a fast clustering method for viral segment lineage determination, applied to the H9 influenza hemagglutinin. Andrew R. Dalby Department of Biomedical Sciences, University of Westminster, 115 New Cavendish Street, Westminster, W1W 6UW, UK …

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  • Templeton, K.E., Scheltinga, S.A., Beersma, M.F., Kroes,

    Templeton, K.E., Scheltinga, S.A., Beersma, M.F., Kroes, A.C. and Claas, E.C. (2004) Rapid and Sensitive Method Using Multiplex Real-Time PCR for Diagnosis of ...

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  • Influenza Virus Testing Methods | CDC

    Table 1: Influenza Virus Testing Methods. Method 1. Types Detected. Acceptable Specimens 2. Test Time. CLIA Waived 3. Rapid Influenza Diagnostic Tests4 (antigen detection) A and B. NP 5 swab, aspirate or wash, nasal swab, aspirate or wash, throat swab.

    Get Price
  • Methods for detecting seasonal influenza epidemics

    2019-9-5 · School absenteeism data have been collected daily by the public health unit in Wellington-Dufferin-Guelph, Ontario since 2008. To date, a threshold-based approach has been implemented to raise alerts for community-wide and within-school illness outbreaks. We investigate several statistical modelling approaches to using school absenteeism for influenza surveillance at the regional level, and ...

    Get Price
  • A Practical Method for Surveillance of Novel H1N1 ...

    We report a surveillance method for influenza that is based on automated hospital laboratory and pharmacy data. During the 2009 H1N1 influenza pandemic, this method was objective, easy to perform, and utilized readily available automated hospital data.

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  • A novel screening method for influenza patients using

    Objectives - In places of mass gathering, rapid infection screening prior to definite diagnosis is vital during the epidemic season of a novel influenza. In order to assess the possibility of clinical application of a newly developed non-contact infection screening system, we conducted screening for influenza patients. Materials and methods - The system is operated by a screening program via a...

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  • Detecting influenza epidemics using search engine query

    2019-10-22 · Detecting influenza epidemics using search engine query data 1 Detecting influenza epidemics using search engine query data Jeremy Ginsberg1, Matthew H. Mohebbi1, Rajan S. Patel1, Lynnette Brammer2, Mark S. Smolinski1 & Larry Brilliant1 1Google Inc. 2Centers for Disease Control and Prevention This paper was originally published in Nature Vol 457, 19 February 2009,

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  • RESEARCH Open Access Novel measurement of

    2017-8-27 · As influenza cases disperse nationwide and an epidemic reaches its peak, WSD value changed to be a progressively increasing. Conclusions: The spatial distribution of nationwide influenza outbreak was measured by using a novel WSD method. We showed that spreading rate varied by type and subtypes of influenza virus using WSD as a spatial indicator.

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  • A multiplex PCR assay for the detection of five influenza ...

    2017-8-29 · influenza viruses using a dual priming oligonucleotide system ... (PCR) is a rapid and sensitive method for the detec-tion of respiratory viruses, such assays are limited to a maximum of four multiplexed targets, and the high cost * Correspondence: [email protected]

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  • Highly sensitive detection of influenza virus in saliva

    2015-9-1 · Biotechnology Reports (2015-09-01) . Highly sensitive detection of influenza virus in saliva by real-time PCR method using sugar chain-immobilized gold nanoparticles; application to clinical studies

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  • Dynamical analysis of the avian–human influenza

    2020-11-29 · In this paper, analytical result of avian–human influenza epidemic model has been investigated by applying homotopy analysis method (HAM) and by expanding it to hybrid numeric-analytic method which is known as multistage HAM (MSHAM).

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  • An easy, rapid, and sensitive method for detection of

    An easy, rapid, and sensitive method for detection of drug-resistant influenza virus by using a sialidase fluorescent imaging probe, BTP3-Neu5Ac . By Daisuke Kato (1827106), Yuuki Kurebayashi (235568), Tadanobu Takahashi (235567), Tadamune Otsubo (508346), Hitomi Otake ...

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  • Using the MEM method for influenza seasonality

    2017-7-26 · Using the MEM method for influenza seasonality determination Dr. Tomás Vega Alonso Dirección General de Salud Pública. Consejería de Sanidad. [email protected] 1 . 2 The problem Influenza: great variation in timing and intensity. Other IRV: multiple causes, overlapping of waves.

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  • WHO information for the molecular detection of

    2017-10-23 · The RT-PCR technique is used as a rapid and sensitive method for the detection of influenza viruses in both clinical samples and isolates. The use of different target gene assays in the RT-PCR is most appropriate for correct identification of this …

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  • An optimized molecular method for detection of

    2020-12-2 · An optimized molecular method for detection of influenza A virus using improved generic primers and concentration of the viral genomic RNA and nucleoprotein complex Ji-Woon Kim,* Chung-Young Lee,* Thanh Trung Nguyen, Il-Hwan Kim, Hyuk-Joon Kwon,1 Jae-Hong Kim Abstract.

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  • Detection of Influenza Virus Infection Using Two PCR

    Rapid, accurate, and cost-effective methods to identify the cause of respiratory tract infections are needed to maximize clinical benefit. Outpatients with acute respiratory illness were tested for influenza using a singleplex reverse transcriptase polymerase chain reaction (SRT-PCR) method. A multiplex RT-PCR (MRT-PCR) method tested for influenza and 17 other viruses and was compared with SRT ...

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  • ELISA method for detection of influenza A infection in

    2021-7-10 · ELISA method for diagnosis of influenza in swine 511 capture ELISA for the direct detection of H1N1 influ-enza virus in swine. This ELISA was compared with the standard egg isolation method in evaluating the duration of virus shedding in swine experimentally infected with a field strain of H1N1. Materials and methods Viruses.

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  • Influenza genome analysis using pyrosequencing

    2018-6-9 · (2009). Influenza genome analysis using pyrosequencing method: current applications for a moving target. Expert Review of Molecular Diagnostics: Vol. 9, No. 5, pp. 493-509.

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  • A multiplex PCR assay for the detection of five influenza ...

    2017-8-29 · RESEARCH ARTICLE Open Access A multiplex PCR assay for the detection of five influenza viruses using a dual priming oligonucleotide system Xuezheng Ma1†, Huanzhou Xu1†, Lei Shi2, Pengfei Yang3, Liping Zhang1, Xiaohong Sun1, Wei Zhen1 and Kongxin Hu1* Abstract

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  • Analytical technologies for influenza virus-like particle ...

    2013-5-4 · Influenza virus-like particle vaccines are one of the most promising ways to respond to the threat of future influenza pandemics. VLPs are composed of viral antigens but lack nucleic acids making them non-infectious which limit the risk of recombination with wild-type strains. By taking advantage of the advancements in cell culture technologies, the process from strain identification to ...

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  • Detecting influenza epidemics using search engine

    2009-2-19 · Previous attempts at using online activity for influenza surveillance have counted search queries submitted to a Swedish medical website (A. Hulth, G. Rydevik and A. Linde, manuscript in ...

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  • Templeton, K.E., Scheltinga, S.A., Beersma, M.F., Kroes,

    Templeton, K.E., Scheltinga, S.A., Beersma, M.F., Kroes, A.C. and Claas, E.C. (2004) Rapid and Sensitive Method Using Multiplex Real-Time PCR for Diagnosis of ...

    Get Price
  • Jiawei Wang - Publications

    Li M, Feng W, Zhao M, Dai P, Zhou Y, Wang J, Lv W, Liu S, Zhao Q. Overcoming Tumor Hypoxia through Multiple Pathways Using an All-in-One Polymeric Therapeutic Agent to Enhance Synergistic Cancer Photo/Chemotherapy Effects. Bioconjugate Chemistry. PMID 34236842 DOI: 10.1021/acs.bioconjchem.1c00307 : 0.88: 2021

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  • Influenza - Therapeutics and Challenges | IntechOpen

    2018-9-19 · Influenza. Therapeutics and Challenges. Edited by Shailendra K. Saxena. King George's Medical University. This book gives a comprehensive overview of recent advances in influenza, as well as general concepts of molecular biology of influenza infections, epidemiology, immunopathology, prevention, and current clinical recommendations in ...

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  • Two Cases of Influenza B Virus-Related Fatal

    Two rare cases of Chinese female patients with influenza B virus infection complicated with both fulminant pneumonia and septicemia caused by Panton–Valentine leukocidin(PVL) positive methicillin-sensitive Staphylococcus aureus (MASS) were reported for the first time in China through next-generation sequencing (NGS). An increasing body of evidence indicates that co-infection with influenza B ...

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  • Symptoms of influenza: how not to miss the first signs

    According to WHO estimates, annual influenza epidemics lead to 3-5 million cases of serious illness and 390-650 thousand deaths. The reconvalescence period is 7-15 days. General information. Currently, more than 2,000 variants of the influenza virus have been identified, which differ in antigenic spectrum.

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  • Blog: Science in the Talmud

    2021-7-14 · We have discussed the value of pi in the Talmud on several previous occasions. But today’s page of Talmud contains one of the important sources on this topic. So let’s take another look at this most magical of numbers, pi, written as the Greek letter π. The Talmud is discussing the minimum legal size of a sukkah, the temporary booth that Jews must live in for seven days each fall.

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  • China Anti-Inflammatory Streptomycin Sulfate ...

    China Anti-Inflammatory Streptomycin Sulfate Pharmaceutical Antibiotic Streptomycin Sulfate Powder Streptomycin Sulfate, Find details about China Streptomycin Sulfate, Streptomycin Sulfate Powder from Anti-Inflammatory Streptomycin Sulfate Pharmaceutical Antibiotic Streptomycin Sulfate Powder Streptomycin Sulfate - Xi`an Disheng Health Biotechnology Co., Ltd.

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  • Temporal Fusion Transformers for Interpretable Multi ...

    2021-7-17 · 纲手对鸣人的惩罚漫画最新章节_纲手对鸣人的惩罚漫画最新 纲手对鸣人的惩罚漫画最新章节_纲手对鸣人的惩罚漫画最新 ...

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  • Ali Salanti - Research outputs - Vaccines, cancer ...

    Temporal Fusion Transformer (TFT) combines attention-based architecture with multi-horizon forecasting with interpretable insights into temporal dynamics. TFT uses recurrent layers for local processing and interpretable self-attention layers for learning long-term dependencies.

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  • Trust me, I'm a chatbot - Dataemia

    The research in the VAR2CSA Team is very diverse and covers vaccines, cancer therapeutics and diagnostic. We focus on vaccines against infectious diseases such as malaria, covid19 and influenza, but also against chronic disease such as cancer, based on novel vaccine platforms developed by the group. Parallel to this the group is pioneering cancer therapeutics, both drug conjugates and immune ...

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  • A novel screening method for influenza patients using

    Objectives - In places of mass gathering, rapid infection screening prior to definite diagnosis is vital during the epidemic season of a novel influenza. In order to assess the possibility of clinical application of a newly developed non-contact infection screening system, we conducted screening for influenza patients. Materials and methods - The system is operated by a screening program via a...

    Get Price
  • A Spatial-Temporal Method to Detect Global Influenza ...

    The 2009 influenza pandemic teaches us how fast the influenza virus could spread globally within a short period of time. To address the challenge of timely global influenza surveillance, this paper presents a spatial-temporal method that incorporates heterogeneous data collected from the Internet to detect influenza epidemics in real time.

    Get Price
  • An optimized molecular method for detection of

    2020-12-2 · An optimized molecular method for detection of influenza A virus using improved generic primers and concentration of the viral genomic RNA and nucleoprotein complex Ji-Woon Kim,* Chung-Young Lee,* Thanh Trung Nguyen, Il-Hwan Kim, Hyuk-Joon Kwon,1 Jae-Hong Kim Abstract.

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  • A method for the formulation of influenza virus

    The relationship of new 'field' isolates of influenza virus to previously isolated strains might not be evident by inspection of haemagglutination-inhibition data. Numerical taxonomic methods have been applied to experimental results in order to clarify these relationships so that antigenic trends may be more easily seen. The diagrammatic representations so derived suggest that numerical...

    Get Price
  • Applying the moving epidemic method to determine

    2020-5-10 · Background Defining the start and assessing the intensity of influenza seasons are essential to ensure timely preventive and control measures and …

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  • Influenza genome analysis using pyrosequencing

    2018-6-9 · (2009). Influenza genome analysis using pyrosequencing method: current applications for a moving target. Expert Review of Molecular Diagnostics: Vol. 9, No. 5, pp. 493-509.

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  • Using an Adjusted Serfling Regression Model to

    2015-3-10 · Serfling-type periodic regression models have been widely used to identify and analyse epidemic of influenza. In these approaches, the baseline is traditionally determined using cleaned historical non-epidemic data. However, we found that the previous exclusion of epidemic seasons was empirical, since year-year variations in the seasonal pattern of activity had been ignored.

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  • A rapid and highly sensitive method for diagnosis of ...

    A rapid and highly sensitive method for diagnosis of equine influenza by detecting viral antigens using immuno-PCR has been developed. The sensitivity of the immuno-PCR for the detection of the nonstructural protein 1 (NS 1) antigenically common among influenza A viruses was 101.0∼2.0 times higher than that of RT-PCR for the detection of the viral genome.

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  • Influenza: Propagation, Quantification, and Storage ...

    2006-12-15 · Influenza viruses are negative-sense, single-stranded, enveloped RNA viruses belonging to the family Orthomyxoviridae. Three types exist, influenza A, B, and C. All infect humans, but only A and B are major human pathogens. Influenza type A viruses are divided into subtypes based on genetic and antigenic differences in the two surface spike ...

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  • EXTRACELLULAR MEASURING METHOD INFLUENZA

    The present invention relates to a method for measuring extracellular influenza virus fusion using a single vesicle containing hemagglutinin and a single vesicle containing sialic acid. More specifically, the single vesicle containing hemagglutinin and fluorescent donor material is produced so as to copy influenza virus, and another single vesicle containing sialic acid and a fluorescent […]

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  • Laboratory Procedures Serological detection of avian ...

    Chinese National Influenza Center, Beijing, has made available attached China, laboratory procedures for serological detection of avian influenza A(H7N9) virus infections by turkey haemagglutination-inhibition assay. 20 December 2013 This assay has been replaced by a modifiedassay using horse red blood cells posted on 2 December0 2013.

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  • Establishing seasonal and alert influenza thresholds in ...

    Objective: To establish seasonal and alert thresholds and transmission intensity categories for influenza to provide timely triggers for preventive measures or upscaling control measures in Cambodia. Methods: Using Cambodia's influenza-like illness (ILI) and laboratory-confirmed influenza surveillance data from 2009 to 2015, three parameters were assessed to monitor influenza activity: the ...

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  • (PDF) Novel measurement of spreading pattern of

    Novel measurement of spreading pattern of influenza epidemic by using weighted standard distance method: retrospective spatial statistical study of influenza, Japan, 1999–2009 By Kiyosu Taniguchi Geographic and Temporal Trends in Influenzalike Illness, Japan, 1992–1999

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  • Automated influenza case detection for public health ...

    An initial study of BCD was conducted for influenza detection using Topaz 22, 25, 26, an NLP tool developed at the University of Pittsburgh, and a high differentiation between influenza cases and non-influenza controls drawn from a low-influenza summer period was found (area under the ROC curve (AUC) for BN-expert classifier: 0.96; AUC for BN ...

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  • [PDF] Pilot study to harmonize the reported influenza ...

    SUMMARY The intensity of annual Spanish influenza activity is currently estimated from historical data of the Spanish Influenza Sentinel Surveillance System (SISSS) using qualitative indicators from the European Influenza Surveillance Network. However, these indicators are subjective, based on qualitative comparison with historical data of influenza-like illness rates. This pilot study ...

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  • A Disease Outbreak Prediction Model Using Bayesian ...

    2021-7-14 · In this method, unknown quantities are considered as additional parameters of the model and are extracted using other parameters. The Markov Chain Monte Carlo algorithm is extensively used in this field. Results: The current study presents a Bayesian statistical analysis of influenza outbreak data using Markov Chain Monte Carlo data

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  • [PDF] Evaluation of A Single-reaction Method for

    OBJECTIVE To evaluate a single-reaction genome amplification method, the multisegment reverse transcription-PCR (M-RTPCR), for its sensitivity to full genome sequencing of influenza A virus, and the ability to differentiate mix-subtype virus, using the next generation sequencing (NGS) platform. METHODS Virus genome copy was quantified and serially diluted to different titers, followed by ...

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  • Technical note “Comparison of influenza virus particle ...

    method, use of the novel magnetic sulfated cellulose particles reduced the influenza A virus particle purification time from 3.5 h to 30 min before mass spectrometry analysis. Small-scale purification of influenza virus particles with cen-trifugation for analytics (CFA) is a time consuming and labor

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